Human CHIP Ubiquitin Ligase Kit - Glow-Fold Substrate

Catalog # Availability Size / Price Qty
K-280
Product Details
Citations (2)
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Human CHIP Ubiquitin Ligase Kit - Glow-Fold Substrate Summary

STIP1 Homology and U-box Containing Protein 1 (STUB1), also known as Carboxyl Terminus of Hsp70-interacting Protein (CHIP), is a cytoplasmic protein that functions as a U-box Ubiquitin ligase (E3). It is highly expressed in striated muscle and brain and has been observed at lower levels in other organs including the pancreas, lung, liver, and kidney. STUB1/CHIP is 303 amino acids (aa) in length with a predicted molecular weight of 34.8 kDa. Human STUB1/CHIP shares 97% aa sequence identity with the mouse ortholog but only 67% sequence identity with the rat ortholog. It consists of three 34 aa N-terminal tetratricopeptide repeat (TRP) domains (aa 26-127) that are responsible for protein-protein interactions and a C-terminal U-box domain (aa 226-300) that participates in ubiquitination. A central domain containing charged residues lies between the TRP and U-box domains and is thought to be necessary for TRP-dependent interactions. STUB1/CHIP participates in intracellular protein folding/refolding and degradation. It complexes with several molecular chaperone proteins, including HSP70/HSPA1A, HSC70, and HSP90, and modulates their activity. It also facilitates the ubiquitination of chaperone substrates, including nascent CFTR, phosphorylated Tau, p53, PTEN, Synuclein-alpha, and beta-APP, promoting their degradation.

Specifications

Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Species
Human

Product Datasheets

Background: CHIP/STUB1

Long Name
C-terminus of Hsc70 Interacting Protein
Entrez Gene IDs
10273 (Human); 56424 (Mouse)
Alternate Names
Antigen NY-CO-7; Carboxy terminus of Hsp70-interacting protein; CHIP; CHIPSTIP1 homology and U box-containing protein 1; CLL-associated antigen KW-8; E3 ubiquitin-protein ligase CHIP; EC 6.3.2.-; heat shock protein A binding protein 2 (c-terminal); HSPABP2; NY-CO-7; SDCCAG7; serologically defined colon cancer antigen 7; STIP1 homology and U-box containing protein 1; STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase; STUB1; UBOX1

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Citations for Human CHIP Ubiquitin Ligase Kit - Glow-Fold Substrate

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. CHIP E3 ligase mediates proteasomal degradation of the proliferation regulatory protein ALDH1L1 during the transition of NIH3T3 fibroblasts from G0/G1 to S-phase
    Authors: QA Khan, P Pediaditak, Y Malakhau, A Esmaeilnia, Z Ashkavand, V Sereda, NI Krupenko, SA Krupenko
    PLoS ONE, 2018;13(7):e0199699.  2018
  2. The E3 Ligase CHIP Mediates p21 Degradation to Maintain Radioresistance
    Authors: K Biswas, S Sarkar, K Du, DL Brautigan, T Abbas, JM Larner
    Mol. Cancer Res, 2017;0(0):.  2017

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