Human cIAP-2/HIAP-1 Biotinylated Antibody

    
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  • Species Reactivity
    Human
  • Specificity
    Detects human cIAP-2/HIAP-1 in Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human cIAP-2/HIAP-1 Asn2-Ser604 Accession # U45878
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of Human cIAP‑2/HIAP‑1 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human cIAP‑2/HIAP‑1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF8171) followed by Streptavidin-HRP (Catalog # DY998). A specific band was detected for cIAP‑2/HIAP‑1 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
      12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Background: cIAP-2/HIAP-1
cIAP-2 (also known as MIHC and HIAP-1) is a member of the inhibitor of apoptosis (IAP) family of proteins that inhibit the proteolytic activity of mature caspases. cIAP-2 has 3 BIR (baculovirus inhibitor of apoptosis) domains, a RING finger domain, and a caspase recruitment domain (CARD). cIAP-2 inhibits caspases through the direct interaction of its BIR domain with the active caspase. Caspase activity may be restored through interactions with the Reaper like motif on mitochondrial proteins such as SMAC/Diablo or HtrA2/Omi. cIAP-2 is reported to be cleaved by HtrA2/Omi.
  • References:
    1. Roy, N. et al. (1997) EMBO J. 23:6914.
    2. Deveraux, Q. et al. (1997) Nature 388:300.
    3. Deveraux, Q. and J. Reed (1999) Genes & Develop. 13:239.
    4. Srinivasula, S.M. et al. (2003) J. Biol. Chem. 278:31469.
    5. Yang, Q-H. et al. (2003) Genes Dev. 17:1487.
  • Entrez Gene IDs:
    330 (Human)
  • Alternate Names:
    AIP1; API2; BIRC3 baculoviral IAP repeat containing 3; BIRC3; cIAP2; c-IAP2; cIAP-2; HAIP1; HIAP1; HIAP-1; MALT2; MIHC; RNF49
Related Research Areas

FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Biotinylated Control

Ctrl BAF108 19
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Reconstitution Buffers
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Reconstitution Buffer 1 (PBS)

RB01
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Staining Reagents
Description Application Cat# Citations Images  

Streptavidin-Alkaline Phosphatase

ELISA(Det) AR001 4
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Streptavidin-Phycoerythrin

Flow F0040 7
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NorthernLights Streptavidin NL557

Flow , IHC , ICC NL999 4
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Streptavidin-Allophycocyanin

Flow F0050 1
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NorthernLights Streptavidin NL637

Flow , IHC , ICC NL998 2
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NorthernLights Streptavidin NL493

Flow , IHC , ICC NL997 2
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Avidin-Fluorescein

Flow F0030 1
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Supplemental Cell Selection Products
Description Application Cat# Citations Images  

MagCellect Streptavidin Ferrofluid

MAG999B 4
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MagCellect Streptavidin Ferrofluid

MAG999 4
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Supplemental ELISA Products
Description Application Cat# Citations Images  

Streptavidin-HRP

ELISA DY998 51  
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EvenCoat Streptavidin Coated Plates

ELISA CP004
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EvenCoat Streptavidin Coated Plates, 5 Pack

ELISA CP003 3
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