cIAP‑2/HIAP‑1 in Human Colon.
cIAP‑2/HIAP‑1 was detected in immersion fixed paraffin-embedded sections of human colon using 15 µg/mL Goat Anti-Human cIAP‑2/HIAP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8171) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the nucleus in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human cIAP‑2/HIAP‑1 by Western Blot.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human cIAP‑2/HIAP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8171) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for cIAP‑2/HIAP‑1 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.
Detection of Human cIAP‑2/HIAP‑1 by Simple WesternTM.
Simple Western lane view shows lysates of Raji human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for cIAP‑2/HIAP‑1 at approximately 70 kDa (as indicated) using 5 µg/mL of Goat Anti-Human cIAP‑2/HIAP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8171) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
cIAP-2 (also known as MIHC and HIAP-1) is a member of the inhibitor of apoptosis (IAP) family of proteins that inhibit the proteolytic activity of mature caspases. cIAP-2 has 3 BIR (baculovirus inhibitor of apoptosis) domains, a RING finger domain, and a caspase recruitment domain (CARD). cIAP-2 inhibits caspases through the direct interaction of its BIR domain with the active caspase. Caspase activity may be restored through interactions with the Reaper like motif on mitochondrial proteins such as SMAC/Diablo or HtrA2/Omi. cIAP-2 is reported to be cleaved by HtrA2/Omi.
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Deveraux, Q. et al. (1997) Nature 388:300.
Deveraux, Q. and J. Reed (1999) Genes & Develop. 13:239.
Srinivasula, S.M. et al. (2003) J. Biol. Chem. 278:31469.
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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Western Blot.
Western Blot: Human cIAP-2/HIAP-1 Antibody [AF8171].
MDA-MB-231 human breast cancer cell line
Other Experimental Details
Other Experimental Details
Western blot analysis of Human cIAP-2/HIAP-1 in MDA-MB-231 cells untreated (A) and treated with 10 uM Paclitaxel for 4 hours (B). Goat anti-Human cIAP-2/HIAP-1antibody (#AF8171) was used at 0.5 ug/mL, followed by Donkey anti-Goat secondary antibody at 1:5,000 dilution.