Human cIAP-2/HIAP-1 Antibody

(8 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human cIAP‑2/HIAP‑1 in Western blots. In Western blots, less than 1% cross-reactivity with cIAP-1 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human cIAP‑2/HIAP‑1
    Asn2-Ser604
    Accession # U45878
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Simple Western
    5 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
cIAP‑2/HIAP‑1 in Human Colon. cIAP‑2/HIAP‑1 was detected in immersion fixed paraffin-embedded sections of human colon using 15 µg/mL Goat Anti-Human cIAP‑2/HIAP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8171) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the nucleus in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human cIAP‑2/HIAP‑1 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human cIAP‑2/HIAP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8171) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for cIAP‑2/HIAP‑1 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.
Detection of Human cIAP‑2/HIAP‑1 by Simple WesternTM. Simple Western lane view shows lysates of Raji human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for cIAP‑2/HIAP‑1 at approximately 70 kDa (as indicated) using 5 µg/mL of Goat Anti-Human cIAP‑2/HIAP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8171) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: cIAP-2/HIAP-1

cIAP-2 (also known as MIHC and HIAP-1) is a member of the inhibitor of apoptosis (IAP) family of proteins that inhibit the proteolytic activity of mature caspases. cIAP-2 has 3 BIR (baculovirus inhibitor of apoptosis) domains, a RING finger domain, and a caspase recruitment domain (CARD). cIAP-2 inhibits caspases through the direct interaction of its BIR domain with the active caspase. Caspase activity may be restored through interactions with the Reaper like motif on mitochondrial proteins such as SMAC/Diablo or HtrA2/Omi. cIAP-2 is reported to be cleaved by HtrA2/Omi.

  • References:
    1. Roy, N. et al. (1997) EMBO J. 23:6914.
    2. Deveraux, Q. et al. (1997) Nature 388:300.
    3. Deveraux, Q. and J. Reed (1999) Genes & Develop. 13:239.
    4. Srinivasula, S.M. et al. (2003) J. Biol. Chem. 278:31469.
    5. Yang, Q-H. et al. (2003) Genes Dev. 17:1487.
  • Entrez Gene IDs:
    330 (Human)
  • Alternate Names:
    AIP1; API2; BIRC3 baculoviral IAP repeat containing 3; BIRC3; cIAP2; c-IAP2; cIAP-2; HAIP1; HIAP1; HIAP-1; MALT2; MIHC; RNF49
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Species
Applications
Sample Type
  1. Anticancer efficacy of the hypoxia-activated prodrug evofosfamide is enhanced in combination with proapoptotic receptor agonists against osteosarcoma
    Authors: V Liapis, A Zysk, M DeNichilo, I Zinonos, S Hay, V Panagopoul, A Shoubridge, C Difelice, V Ponomarev, W Ingman, GJ Atkins, DM Findlay, ACW Zannettino, A Evdokiou
    Cancer Med, 2017;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Pro-Apoptotic Activity of New Honokiol/Triphenylmethane Analogues in B-Cell Lymphoid Malignancies
    Molecules, 2016;21(8):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. USP11-dependent selective cIAP2 deubiquitylation and stabilization determine sensitivity to Smac mimetics.
    Authors: Lee E, Seong D, Seo J, Jeong M, Lee H, Song J
    Cell Death Differ, 2015;22(9):1463-76.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. Doxorubicin overcomes resistance to drozitumab by antagonizing Inhibitor of Apoptosis Proteins (IAPs).
    Authors: Zinonos I, Labrinidis A, Liapis V, Hay S, Panagopoulos V, Denichilo M, Ponomarev V, Ingman W, Atkins G, Findlay D, Zannettino A, Evdokiou A
    Anticancer Res, 2014;34(12):7007-20.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. The novel SMAC mimetic birinapant exhibits potent activity against human melanoma cells.
    Authors: Krepler C, Chunduru S, Halloran M, He X, Xiao M, Vultur A, Villanueva J, Mitsuuchi Y, Neiman E, Benetatos C, Nathanson K, Amaravadi R, Pehamberger H, McKinlay M, Herlyn M
    Clin Cancer Res, 2013;19(7):1784-94.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
  6. Dacarbazine and the agonistic TRAIL receptor-2 antibody lexatumumab induce synergistic anticancer effects in melanoma.
    Authors: Engesaeter B, Engebraaten O, Florenes V, Maelandsmo G
    PLoS ONE, 2012;7(9):e45492.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  7. cIAP1 Localizes to the nuclear compartment and modulates the cell cycle.
    Authors: Samuel T, Okada K, Hyer M, Welsh K, Zapata JM, Reed JC
    Cancer Res., 2005;65(1):210-8.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  8. Survivin interacts with Smac/DIABLO in ovarian carcinoma cells but is redundant in Smac-mediated apoptosis.
    Authors: McNeish IA, Lopes R, Bell SJ, McKay TR, Fernandez M, Lockley M, Wheatley SP, Lemoine NR
    Exp. Cell Res., 2005;302(1):69-82.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
Cell and Tissue Staining Kits
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Immunohistochemistry Reagents
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Isotype Controls
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Normal Goat IgG Control

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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

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Goat IgG (H+L) APC-conjugated Antibody

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Goat IgG Horseradish Peroxidase-conjugated Antibody

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Donkey Anti-Goat IgG Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

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Chicken Anti-Goat IgG Biotinylated Antibody

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Rabbit Anti-Goat IgG Biotinylated Antibody

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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Western Blot.

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Images Ratings Applications Species Reviewed By Date Details
Western Blot Human cIAP-2/HIAP-1 Antibody AF8171
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 WB Human Anonymous 03/27/2017
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Western Blot Human cIAP-2/HIAP-1 Antibody AF8171
Western Blot: Human cIAP-2/HIAP-1 Antibody [AF8171]

Summary

ApplicationWestern Blot
Sample TestedMDA-MB-231 human breast cancer cell line
SpeciesHuman

Other Experimental Details

Other Experimental DetailsWestern blot analysis of Human cIAP-2/HIAP-1 in MDA-MB-231 cells untreated (A) and treated with 10 uM Paclitaxel for 4 hours (B). Goat anti-Human cIAP-2/HIAP-1antibody (#AF8171) was used at 0.5 ug/mL, followed by Donkey anti-Goat secondary antibody at 1:5,000 dilution.

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