Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Blockade of Receptor-ligand Interaction, Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, ELISA Capture

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CL-P1/COLEC12 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CL‑P1/COLEC12 isoform 1
Leu57-Leu742
Accession # Q5KU26

Specificity

Detects CL‑P1/COLEC12 in direct ELISAs and Western blots. In direct ELISAs, approximately 45% cross‑reactivity with recombinant mouse CL-P1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human CL‑P1/COLEC12 Antibody

Detection of CL-P1/COLEC12 antibody in HUVEC Human Cells antibody by Flow Cytometry.

Detection of CL‑P1/COLEC12 in HUVEC Human Cells by Flow Cytometry.

HUVEC human umbilical vein endothelial cells were stained with Goat Anti-Human CL-P1/COLEC12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2690, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

Detection of Human CL-P1/COLEC12 by Immunocytochemistry/Immunofluorescence

Detection of Human CL-P1/COLEC12 by Immunocytochemistry/Immunofluorescence

MCAM and COLEC12 are novel BEC- and LEC- specific markers.Two new endothelial markers MCAM and COLEC12 (selected from our analysis of Dataset B) were used to stain (A) normal human lymph nodes together with two established vascular markers PV-1 (for BEC) and CLEVER-1 (for LEC). In addition, (B) chronically inflamed tonsils and (C) specimens from bladder cancer and colorectal cancer were stained with the antibodies against the indicated proteins. LYVE-1 and COLEC12 co-staining was done on colorectal cancer specimens, whereas the other stainings represent bladder cancer. MCAM staining colocalized very well with the established BEC marker PV-1 whereas no colocalization could be detected between MCAM and the established LEC markers LYVE-1 and podoplanin. COLEC12-staining on the other hand showed colocalization with LYVE-1 but not PV-1. White arrows point to areas of colocalization. Nuclear counterstaining was performed with DAPI. Scale bars represent 100 µm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24058540), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human CL-P1/COLEC12 by Immunocytochemistry/Immunofluorescence

Detection of Human CL-P1/COLEC12 by Immunocytochemistry/Immunofluorescence

MCAM and COLEC12 are novel BEC- and LEC- specific markers.Two new endothelial markers MCAM and COLEC12 (selected from our analysis of Dataset B) were used to stain (A) normal human lymph nodes together with two established vascular markers PV-1 (for BEC) and CLEVER-1 (for LEC). In addition, (B) chronically inflamed tonsils and (C) specimens from bladder cancer and colorectal cancer were stained with the antibodies against the indicated proteins. LYVE-1 and COLEC12 co-staining was done on colorectal cancer specimens, whereas the other stainings represent bladder cancer. MCAM staining colocalized very well with the established BEC marker PV-1 whereas no colocalization could be detected between MCAM and the established LEC markers LYVE-1 and podoplanin. COLEC12-staining on the other hand showed colocalization with LYVE-1 but not PV-1. White arrows point to areas of colocalization. Nuclear counterstaining was performed with DAPI. Scale bars represent 100 µm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24058540), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human CL-P1/COLEC12 by Immunocytochemistry/Immunofluorescence

Detection of Human CL-P1/COLEC12 by Immunocytochemistry/Immunofluorescence

MCAM and COLEC12 are novel BEC- and LEC- specific markers.Two new endothelial markers MCAM and COLEC12 (selected from our analysis of Dataset B) were used to stain (A) normal human lymph nodes together with two established vascular markers PV-1 (for BEC) and CLEVER-1 (for LEC). In addition, (B) chronically inflamed tonsils and (C) specimens from bladder cancer and colorectal cancer were stained with the antibodies against the indicated proteins. LYVE-1 and COLEC12 co-staining was done on colorectal cancer specimens, whereas the other stainings represent bladder cancer. MCAM staining colocalized very well with the established BEC marker PV-1 whereas no colocalization could be detected between MCAM and the established LEC markers LYVE-1 and podoplanin. COLEC12-staining on the other hand showed colocalization with LYVE-1 but not PV-1. White arrows point to areas of colocalization. Nuclear counterstaining was performed with DAPI. Scale bars represent 100 µm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24058540), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human CL‑P1/COLEC12 Antibody

Application
Recommended Usage

Blockade of Receptor-ligand Interaction

In a functional ELISA, 2-8 µg/mL of this antibody will block 50% of the binding of 250 ng/mL of biotinylated AGE‑BSA to immobilized Recombinant Human CL-P1/COLEC12 (Catalog # 2690-CL) coated at 5 µg/mL (100 µL/well). At 100 μg/mL, this antibody will block >90% of the binding.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: HUVEC human umbilical vein endothelial cells

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells

Western Blot

0.1 µg/mL
Sample: Recombinant Human CL-P1/COLEC12 (Catalog # 2690-CL)

Reviewed Applications

Read 1 review rated 5 using AF2690 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CL-P1/COLEC12

Collectins are a family of Ca++-dependent, C-type lectins that contain a collagenous domain and function as recognition molecules for molecular patterns found on pathogens (1‑4). Human collectin placenta 1 (CL-P1; also known as collectin sub-family member 12 and SRCL type I [scavenger receptor with C-type lectin type I]) is a 110 kDa member of the collectin family of glycoproteins (5, 6). CL-P1 is the only collectin known to be membrane bound, while CL-L1 (collectin liver-1) is the only known cytoplasmic collectin (1). Human CL-P1 is synthesized as a 742 amino acid (aa) type II transmembrane glycoprotein that contains an N-terminal 39 aa cytoplasmic domain, a 17 aa transmembrane segment, and a 686 aa C-terminal extracellular region (6). The short cytoplasmic domain contains an internalization motif (Y-K-R-F) while the extracellular region is complex, demonstrating a coiled-coil segment, a Ser-Thr rich region, a collagen-like structure and a C-type lectin/ carbohydrate recognition domain (CRD). Notably, this CRD recognizes galactose (and fucose) within the context of asialo‑orosomucoids associated with the Lewisx epitope (7, 8). CL-P1 has a 300 kDa trimeric form due to its collagen-like and coiled-coil helical domains (1, 5). There is a 97 kDa, alternate splice form of CL-P1 (SRCL type II) that shows a 120 aa truncation at the C-terminus. This effectively removes the entire CRD found on full‑length CL-P1 (6). Human CL-P1 is 93% aa identical to mouse CL-P1 over the entire extracellular region, and 87% aa identical over the CRD region (5, 9). Human CL-P1 is known to be expressed in vascular endothelial cells (5).

References

  1. van de Wetering, JK. et al. (2004) Eur. J. Biochem. 271:1229.
  2. Holmskov, U. et al. (2003) Annu. Rev. Immunol. 21:547.
  3. Hoppe, H-J. and K. Reid (1994) Protein Sci. 3:1143.
  4. Hickling, T.P. et al. (2004) J. Leukoc. Biol. 75:27.
  5. Ohtani, K. et al. (2001) J. Biol. Chem. 276:44222.
  6. Nakamura,K. et al. (2001) Biochem. Biophys. Res. Commun. 280:1028.
  7. Coombs, P.J. et al. (2005) J. Biol. Chem. 280:22993.
  8. Yoshida, T. et al. (2003) J. Biochem. 133:271.
  9. Nakamura, K. et al. (2001) Biochim. Biophys. Acta 1522:53.

Long Name

Collectin Placenta 1

Alternate Names

COLEC12, NSR2, SCARA4, SRCL Type I

Entrez Gene IDs

81035 (Human); 140792 (Mouse); 361289 (Rat)

Gene Symbol

COLEC12

UniProt

Q5KU26

Additional CL-P1/COLEC12 Products

Product Documents for Human CL‑P1/COLEC12 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CL‑P1/COLEC12 Antibody

For research use only

Citations for Human CL‑P1/COLEC12 Antibody

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    Application: ELISA
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 09/18/2018
    Human CL‑P1/COLEC12 Antibody AF2690

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