Recombinant Human CL-P1/COLEC12 (Catalog # 2690-CL)
2.5 µg/106 cells
Blockade of Receptor-ligand Interaction
In a functional ELISA, 2-8 µg/mL of this antibody will block 50% of the binding of 250 ng/mL of biotinylated AGE‑BSA to immobilized Recombinant Human CL-P1/COLEC12 (Catalog # 2690-CL) coated at 5 µg/mL (100 µL/well). At 100 μg/mL, this antibody will block >90% of the binding.
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immersion fixed HUVEC human umbilical vein endothelial cells
Please Note: Optimal dilutions should be determined by each laboratory for each application.
are available in the Technical Information section on our website.
Detection of CL‑P1/COLEC12 in HUVEC Human Cells by Flow Cytometry.
HUVEC human umbilical vein endothelial cells were stained with Goat Anti-Human CL‑P1/COLEC12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2690, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Collectins are a family of Ca++-dependent, C-type lectins that contain a collagenous domain and function as recognition molecules for molecular patterns found on pathogens (1‑4). Human collectin placenta 1 (CL-P1; also known as collectin sub-family member 12 and SRCL type I [scavenger receptor with C-type lectin type I]) is a 110 kDa member of the collectin family of glycoproteins (5, 6). CL-P1 is the only collectin known to be membrane bound, while CL-L1 (collectin liver-1) is the only known cytoplasmic collectin (1). Human CL-P1 is synthesized as a 742 amino acid (aa) type II transmembrane glycoprotein that contains an N-terminal 39 aa cytoplasmic domain, a 17 aa transmembrane segment, and a 686 aa C-terminal extracellular region (6). The short cytoplasmic domain contains an internalization motif (Y-K-R-F) while the extracellular region is complex, demonstrating a coiled-coil segment, a Ser-Thr rich region, a collagen-like structure and a C-type lectin/ carbohydrate recognition domain (CRD). Notably, this CRD recognizes galactose (and fucose) within the context of asialo‑orosomucoids associated with the Lewisx epitope (7, 8). CL-P1 has a 300 kDa trimeric form due to its collagen-like and coiled-coil helical domains (1, 5). There is a 97 kDa, alternate splice form of CL-P1 (SRCL type II) that shows a 120 aa truncation at the C-terminus. This effectively removes the entire CRD found on full‑length CL-P1 (6). Human CL-P1 is 93% aa identical to mouse CL-P1 over the entire extracellular region, and 87% aa identical over the CRD region (5, 9). Human CL-P1 is known to be expressed in vascular endothelial cells (5).
van de Wetering, JK. et al. (2004) Eur. J. Biochem. 271:1229.
Holmskov, U. et al. (2003) Annu. Rev. Immunol. 21:547.
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Coombs, P.J. et al. (2005) J. Biol. Chem. 280:22993.
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Nakamura, K. et al. (2001) Biochim. Biophys. Acta 1522:53.
Collectin Placenta 1
Entrez Gene IDs:
81035 (Human); 140792 (Mouse); 361289 (Rat)
CL-P1; CLP1, cleavage and polyadenylation factor I subunit, homolog (S. cerevisiae); COLEC12; EC 126.96.36.199; hClp1; HEABATP/GTP-binding protein; homolog of yeast CFIA subunit Clp1p; NSR2; Polynucleotide kinase Clp1; polyribonucleotide 5'-hydroxyl-kinase Clp1; Pre-mRNA cleavage complex II protein Clp1; SCARA4; SRCL Type I
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