Human CL-P1/COLEC12 Antibody

Name: Human CL-P1/COLEC12 Antibody
Brand: R&D Systems
SKU: AF2690-SP
Rating: 5 (1 reviews)

Catalog #: AF2690
4 Citations 1 Review Datasheet / COA / SDS

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AF2690
AF2690-SP

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Detection of CL‑P1/COLEC12 in HUVEC Human Cells by Flow Cytometry.

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Citations (4)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CL-P1/COLEC12 Antibody Summary

Species Reactivity

Human

Specificity

Detects CL‑P1/COLEC12 in direct ELISAs and Western blots. In direct ELISAs, approximately 45% cross‑reactivity with recombinant mouse CL-P1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CL‑P1/COLEC12 isoform 1
Leu57-Leu742
Accession # Q5KU26

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human CL-P1/COLEC12 (Catalog # 2690-CL)

Flow Cytometry

2.5 µg/10⁶ cells

See below

Blockade of Receptor-ligand Interaction

In a functional ELISA, 2-8 µg/mL of this antibody will block 50% of the binding of 250 ng/mL of biotinylated AGE‑BSA to immobilized Recombinant Human CL-P1/COLEC12 (Catalog # 2690-CL) coated at 5 µg/mL (100 µL/well). At 100 μg/mL, this antibody will block >90% of the binding.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

5-15 µg/mL

Immersion fixed HUVEC human umbilical vein endothelial cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CL‑P1/COLEC12 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Goat Anti-Human CL-P1/COLEC12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2690, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CL-P1/COLEC12

Collectins are a family of Ca⁺⁺-dependent, C-type lectins that contain a collagenous domain and function as recognition molecules for molecular patterns found on pathogens (1‑4). Human collectin placenta 1 (CL-P1; also known as collectin sub-family member 12 and SRCL type I [scavenger receptor with C-type lectin type I]) is a 110 kDa member of the collectin family of glycoproteins (5, 6). CL-P1 is the only collectin known to be membrane bound, while CL-L1 (collectin liver-1) is the only known cytoplasmic collectin (1). Human CL-P1 is synthesized as a 742 amino acid (aa) type II transmembrane glycoprotein that contains an N-terminal 39 aa cytoplasmic domain, a 17 aa transmembrane segment, and a 686 aa C-terminal extracellular region (6). The short cytoplasmic domain contains an internalization motif (Y-K-R-F) while the extracellular region is complex, demonstrating a coiled-coil segment, a Ser-Thr rich region, a collagen-like structure and a C-type lectin/ carbohydrate recognition domain (CRD). Notably, this CRD recognizes galactose (and fucose) within the context of asialo‑orosomucoids associated with the Lewis^x epitope (7, 8). CL-P1 has a 300 kDa trimeric form due to its collagen-like and coiled-coil helical domains (1, 5). There is a 97 kDa, alternate splice form of CL-P1 (SRCL type II) that shows a 120 aa truncation at the C-terminus. This effectively removes the entire CRD found on full‑length CL-P1 (6). Human CL-P1 is 93% aa identical to mouse CL-P1 over the entire extracellular region, and 87% aa identical over the CRD region (5, 9). Human CL-P1 is known to be expressed in vascular endothelial cells (5).

References

van de Wetering, JK. et al. (2004) Eur. J. Biochem. 271:1229.
Holmskov, U. et al. (2003) Annu. Rev. Immunol. 21:547.
Hoppe, H-J. and K. Reid (1994) Protein Sci. 3:1143.
Hickling, T.P. et al. (2004) J. Leukoc. Biol. 75:27.
Ohtani, K. et al. (2001) J. Biol. Chem. 276:44222.
Nakamura,K. et al. (2001) Biochem. Biophys. Res. Commun. 280:1028.
Coombs, P.J. et al. (2005) J. Biol. Chem. 280:22993.
Yoshida, T. et al. (2003) J. Biochem. 133:271.
Nakamura, K. et al. (2001) Biochim. Biophys. Acta 1522:53.

Long Name

Collectin Placenta 1

Entrez Gene IDs

81035 (Human); 140792 (Mouse); 361289 (Rat)

Alternate Names

CL-P1; CLP1hCL-P1; COLEC12; collectin placenta 1; Collectin placenta protein 1; collectin sub-family member 12; collectin-12; NSR2; Nurse cell scavenger receptor 2; SCARA4; SCARA4NSR2; Scavenger receptor class A member 4; scavenger receptor class A, member 4; SRCL Type I; SRCLScavenger receptor with C-type lectin

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human CL-P1/COLEC12 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Rapid and Efficient Purification of Functional Collectin-12 and Its Opsonic Activity against Fungal Pathogens
Authors: J Zhang, A Li, CQ Yang, P Garred, YJ Ma
J Immunol Res, 2019;2019(0):9164202.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot
Scavenger receptor collectin placenta 1 is a novel receptor involved in the uptake of myelin by phagocytes
Authors: JF Bogie, J Mailleux, E Wouters, W Jorissen, E Grajchen, J Vanmol, K Wouters, N Hellings, J Van Horsen, T Vanmierlo, JJ Hendriks
Sci Rep, 2017;7(0):44794.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Soluble Collectin-12 (CL-12) Is a Pattern Recognition Molecule Initiating Complement Activation via the Alternative Pathway.
Authors: Ma Y, Hein E, Munthe-Fog L, Skjoedt M, Bayarri-Olmos R, Romani L, Garred P
J Immunol, 2015;195(7):3365-73.
Species: N/A
Sample Types: Whole Cells
Applications: Flow Cytometry
Plasticity of blood- and lymphatic endothelial cells and marker identification.
Authors: Keuschnigg, Johannes, Karinen, Sirkku, Auvinen, Kaisa, Irjala, Heikki, Mpindi, John-Pat, Kallioniemi, Olli, Hautaniemi, Sampsa, Jalkanen, Sirpa, Salmi, Marko
PLoS ONE, 2013;8(9):e74293.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr