Human CL-P1/COLEC12 Antibody

(3 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects CL‑P1/COLEC12 in direct ELISAs and Western blots. In direct ELISAs, approximately 45% cross‑reactivity with recombinant mouse CL-P1 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human CL‑P1/COLEC12 isoform 1
    Leu57-Leu742
    Accession # Q5KU26
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human CL-P1/COLEC12 (Catalog # 2690-CL)
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • Blockade of Receptor-ligand Interaction
    In a functional ELISA, 2-8 µg/mL of this antibody will block 50% of the binding of 250 ng/mL of biotinylated AGE‑BSA to immobilized Recombinant Human CL-P1/COLEC12 (Catalog # 2690-CL) coated at 5 µg/mL (100 µL/well). At 100 μg/mL, this antibody will block >90% of the binding.
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    5-15 µg/mL
    Immersion fixed HUVEC human umbilical vein endothelial cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CL‑P1/COLEC12 in HUVEC Human Cells by Flow Cytometry.

HUVEC human umbilical vein endothelial cells were stained with Goat Anti-Human CL‑P1/COLEC12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2690, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CL-P1/COLEC12

Collectins are a family of Ca++-dependent, C-type lectins that contain a collagenous domain and function as recognition molecules for molecular patterns found on pathogens (1‑4). Human collectin placenta 1 (CL-P1; also known as collectin sub-family member 12 and SRCL type I [scavenger receptor with C-type lectin type I]) is a 110 kDa member of the collectin family of glycoproteins (5, 6). CL-P1 is the only collectin known to be membrane bound, while CL-L1 (collectin liver-1) is the only known cytoplasmic collectin (1). Human CL-P1 is synthesized as a 742 amino acid (aa) type II transmembrane glycoprotein that contains an N-terminal 39 aa cytoplasmic domain, a 17 aa transmembrane segment, and a 686 aa C-terminal extracellular region (6). The short cytoplasmic domain contains an internalization motif (Y-K-R-F) while the extracellular region is complex, demonstrating a coiled-coil segment, a Ser-Thr rich region, a collagen-like structure and a C-type lectin/ carbohydrate recognition domain (CRD). Notably, this CRD recognizes galactose (and fucose) within the context of asialo‑orosomucoids associated with the Lewisx epitope (7, 8). CL-P1 has a 300 kDa trimeric form due to its collagen-like and coiled-coil helical domains (1, 5). There is a 97 kDa, alternate splice form of CL-P1 (SRCL type II) that shows a 120 aa truncation at the C-terminus. This effectively removes the entire CRD found on full‑length CL-P1 (6). Human CL-P1 is 93% aa identical to mouse CL-P1 over the entire extracellular region, and 87% aa identical over the CRD region (5, 9). Human CL-P1 is known to be expressed in vascular endothelial cells (5).

  • References:
    1. van de Wetering, JK. et al. (2004) Eur. J. Biochem. 271:1229.
    2. Holmskov, U. et al. (2003) Annu. Rev. Immunol. 21:547.
    3. Hoppe, H-J. and K. Reid (1994) Protein Sci. 3:1143.
    4. Hickling, T.P. et al. (2004) J. Leukoc. Biol. 75:27.
    5. Ohtani, K. et al. (2001) J. Biol. Chem. 276:44222.
    6. Nakamura,K. et al. (2001) Biochem. Biophys. Res. Commun. 280:1028.
    7. Coombs, P.J. et al. (2005) J. Biol. Chem. 280:22993.
    8. Yoshida, T. et al. (2003) J. Biochem. 133:271.
    9. Nakamura, K. et al. (2001) Biochim. Biophys. Acta 1522:53.
  • Long Name:
    Collectin Placenta 1
  • Entrez Gene IDs:
    81035 (Human); 140792 (Mouse); 361289 (Rat)
  • Alternate Names:
    CL-P1; CLP1, cleavage and polyadenylation factor I subunit, homolog (S. cerevisiae); COLEC12; EC 2.7.1.78; hClp1; HEABATP/GTP-binding protein; homolog of yeast CFIA subunit Clp1p; NSR2; Polynucleotide kinase Clp1; polyribonucleotide 5'-hydroxyl-kinase Clp1; Pre-mRNA cleavage complex II protein Clp1; SCARA4; SRCL Type I
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Scavenger receptor collectin placenta 1 is a novel receptor involved in the uptake of myelin by phagocytes
    Authors: JF Bogie, J Mailleux, E Wouters, W Jorissen, E Grajchen, J Vanmol, K Wouters, N Hellings, J Van Horsen, T Vanmierlo, JJ Hendriks
    Sci Rep, 2017;7(0):44794.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Soluble Collectin-12 (CL-12) Is a Pattern Recognition Molecule Initiating Complement Activation via the Alternative Pathway.
    Authors: Ma Y, Hein E, Munthe-Fog L, Skjoedt M, Bayarri-Olmos R, Romani L, Garred P
    J Immunol, 2015;195(7):3365-73.
    Species: N/A
    Sample Type: Whole Cells
    Application: Flow
  3. Plasticity of blood- and lymphatic endothelial cells and marker identification.
    Authors: Keuschnigg, Johannes, Karinen, Sirkku, Auvinen, Kaisa, Irjala, Heikki, Mpindi, John-Pat, Kallioniemi, Olli, Hautaniemi, Sampsa, Jalkanen, Sirpa, Salmi, Marko
    PLoS ONE, 2013;8(9):e74293.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
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