Human CL-P1/COLEC12 Antibody Summary
Accession # Q5KU26
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CL‑P1/COLEC12 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Goat Anti-Human CL-P1/COLEC12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2690, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Collectins are a family of Ca++-dependent, C-type lectins that contain a collagenous domain and function as recognition molecules for molecular patterns found on pathogens (1‑4). Human collectin placenta 1 (CL-P1; also known as collectin sub-family member 12 and SRCL type I [scavenger receptor with C-type lectin type I]) is a 110 kDa member of the collectin family of glycoproteins (5, 6). CL-P1 is the only collectin known to be membrane bound, while CL-L1 (collectin liver-1) is the only known cytoplasmic collectin (1). Human CL-P1 is synthesized as a 742 amino acid (aa) type II transmembrane glycoprotein that contains an N-terminal 39 aa cytoplasmic domain, a 17 aa transmembrane segment, and a 686 aa C-terminal extracellular region (6). The short cytoplasmic domain contains an internalization motif (Y-K-R-F) while the extracellular region is complex, demonstrating a coiled-coil segment, a Ser-Thr rich region, a collagen-like structure and a C-type lectin/ carbohydrate recognition domain (CRD). Notably, this CRD recognizes galactose (and fucose) within the context of asialo‑orosomucoids associated with the Lewisx epitope (7, 8). CL-P1 has a 300 kDa trimeric form due to its collagen-like and coiled-coil helical domains (1, 5). There is a 97 kDa, alternate splice form of CL-P1 (SRCL type II) that shows a 120 aa truncation at the C-terminus. This effectively removes the entire CRD found on full‑length CL-P1 (6). Human CL-P1 is 93% aa identical to mouse CL-P1 over the entire extracellular region, and 87% aa identical over the CRD region (5, 9). Human CL-P1 is known to be expressed in vascular endothelial cells (5).
- van de Wetering, JK. et al. (2004) Eur. J. Biochem. 271:1229.
- Holmskov, U. et al. (2003) Annu. Rev. Immunol. 21:547.
- Hoppe, H-J. and K. Reid (1994) Protein Sci. 3:1143.
- Hickling, T.P. et al. (2004) J. Leukoc. Biol. 75:27.
- Ohtani, K. et al. (2001) J. Biol. Chem. 276:44222.
- Nakamura,K. et al. (2001) Biochem. Biophys. Res. Commun. 280:1028.
- Coombs, P.J. et al. (2005) J. Biol. Chem. 280:22993.
- Yoshida, T. et al. (2003) J. Biochem. 133:271.
- Nakamura, K. et al. (2001) Biochim. Biophys. Acta 1522:53.
Citations for Human CL-P1/COLEC12 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 4
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Rapid and Efficient Purification of Functional Collectin-12 and Its Opsonic Activity against Fungal Pathogens
Authors: J Zhang, A Li, CQ Yang, P Garred, YJ Ma
J Immunol Res, 2019;2019(0):9164202.
Sample Types: Cell Culture Supernates
Applications: Western Blot
Scavenger receptor collectin placenta 1 is a novel receptor involved in the uptake of myelin by phagocytes
Authors: JF Bogie, J Mailleux, E Wouters, W Jorissen, E Grajchen, J Vanmol, K Wouters, N Hellings, J Van Horsen, T Vanmierlo, JJ Hendriks
Sci Rep, 2017;7(0):44794.
Sample Types: Cell Lysates
Applications: Western Blot
Soluble Collectin-12 (CL-12) Is a Pattern Recognition Molecule Initiating Complement Activation via the Alternative Pathway.
Authors: Ma Y, Hein E, Munthe-Fog L, Skjoedt M, Bayarri-Olmos R, Romani L, Garred P
J Immunol, 2015;195(7):3365-73.
Sample Types: Whole Cells
Applications: Flow Cytometry
Plasticity of blood- and lymphatic endothelial cells and marker identification.
Authors: Keuschnigg, Johannes, Karinen, Sirkku, Auvinen, Kaisa, Irjala, Heikki, Mpindi, John-Pat, Kallioniemi, Olli, Hautaniemi, Sampsa, Jalkanen, Sirpa, Salmi, Marko
PLoS ONE, 2013;8(9):e74293.
Sample Types: Whole Tissue
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