Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair)

Cited:

Proximity Ligation Assay (PLA)

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all Coagulation Factor III/Tissue Factor Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Coagulation Factor III/Tissue Factor (R&D Systems, Catalog # 2339-PA)
Gly34-Glu251
Accession # P13726

Specificity

Detects human Coagulation Factor III/Tissue Factor in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with human Plasminogen, recombinant human (rh) Coagulation Factor VII, rhCoagulation Factor X, rhCoagulation Factor XI, rhCoagulation Factor XIV and recombinant mouse Coagulation Factor III/Tissue Factor is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Coagulation Factor III/Tissue Factor Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Coagulation Factor III/Tissue Factor (Catalog # 2339-PA)

Human Coagulation Factor III/Tissue Factor Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human Coagulation Factor III/Tissue Factor Antibody (Catalog # MAB2339)
  • Standard: Recombinant Human Coagulation Factor III/Tissue Factor, CF (Catalog # 2339-PA)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using BAF2339 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Coagulation Factor III/Tissue Factor

Coagulation Factor III/Tissue Factor (TF), also known as thromboplastin and CD142, is an integral membrane protein found in a variety of cell types. It functions as a protein cofactor/receptor of Coagulation Factor VII, which is synthesized in the liver and circulated in the plasma (1). Upon binding of TF, the inactive factor VII is rapidly converted into activated VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and has also important coagulation-independent functions such as angiognesis (2). Synthesized as a 295 amino acid precursor, TF consists of a signal peptide (residues 1-32) and the mature chain (residues 33-295). As a type I membrane protein, it contains a transmembrane region (residues 252-274) and a cytoplasmic tail (residues 275-295) (3-6).

References

  1. Morrissey, J.H. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (ed) San Diego, Academic Press, p. 1659. 
  2. Versteeg, H.H. et al. (2003) Carcinogenesis 24:1009. 
  3. Scarpati, E.M. et al. (1987) Biochemistry 26:5234.
  4. Fisher, K.L. et al. (1987) Thromb. Res. 48:89.
  5. Morrissey, J.H. et al. (1987) Cell 50:129.
  6. Spicer, E.K. (1987) Proc. Natl. Acad. Sci. USA 84:5148.

Alternate Names

CD142, F3, Thromboplastin, Tissue Factor

Entrez Gene IDs

2152 (Human); 14066 (Mouse)

Gene Symbol

F3

UniProt

P13726

Additional Coagulation Factor III/Tissue Factor Products

Product Documents for Human Coagulation Factor III/Tissue Factor Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Coagulation Factor III/Tissue Factor Biotinylated Antibody

For research use only

Citations for Human Coagulation Factor III/Tissue Factor Biotinylated Antibody

Customer Reviews for Human Coagulation Factor III/Tissue Factor Biotinylated Antibody (1)

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  • Human Coagulation Factor III/Tissue Factor Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 07/06/2021
    we used this antibody for an ELISA to quantify human serum tissue factor H, works very well.
    Human Coagulation Factor III/Tissue Factor Biotinylated Antibody BAF2339

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