Coagulation Factor III/Tissue Factor (TF), also known as thromboplastin and CD142, is an integral membrane protein found in a variety of cell types. It functions as a protein cofactor/receptor of Coagulation Factor VII, which is synthesized in the liver and circulated in the plasma (1). Upon binding of TF, the inactive factor VII is rapidly converted into activated VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and has also important coagulation-independent functions such as angiognesis (2). Synthesized as a 295 amino acid precursor, TF consists of a signal peptide (residues 1-32) and the mature chain (residues 33-295). As a type I membrane protein, it contains a transmembrane region (residues 252-274) and a cytoplasmic tail (residues 275-295) (3-6).
Human Coagulation Factor III/Tissue Factor Antibody [Biotin]
R&D Systems | Catalog # BAF2339
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Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot, ELISA Detection (Matched Antibody Pair)
Cited:
Proximity Ligation Assay (PLA)
Label
Biotin
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Coagulation Factor III/Tissue Factor (R&D Systems, Catalog # 2339-PA)
Gly34-Glu251
Accession # P13726
Gly34-Glu251
Accession # P13726
Specificity
Detects human Coagulation Factor III/Tissue Factor in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with human Plasminogen, recombinant human (rh) Coagulation Factor VII, rhCoagulation Factor X, rhCoagulation Factor XI, rhCoagulation Factor XIV and recombinant mouse Coagulation Factor III/Tissue Factor is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Coagulation Factor III/Tissue Factor Antibody [Biotin]
Human Coagulation Factor III / Tissue Factor ELISA Standard Curve
Recombinant Human Coagulation Factor III/Tissue Factor (Catalog # 2339-PA) was serially diluted and captured by Mouse Anti-Human Coagulation Factor III/Tissue Factor Monoclonal Antibody (Catalog # MAB2339) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Coagulation Factor III/Tissue Factor Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2339) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Human Coagulation Factor III/Tissue Factor Antibody [Biotin]
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human Coagulation Factor III/Tissue Factor (Catalog # 2339-PA)
Sample: Recombinant Human Coagulation Factor III/Tissue Factor (Catalog # 2339-PA)
Human Coagulation Factor III/Tissue Factor Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using BAF2339 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Coagulation Factor III/Tissue Factor
References
- Morrissey, J.H. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (ed) San Diego, Academic Press, p. 1659.
- Versteeg, H.H. et al. (2003) Carcinogenesis 24:1009.
- Scarpati, E.M. et al. (1987) Biochemistry 26:5234.
- Fisher, K.L. et al. (1987) Thromb. Res. 48:89.
- Morrissey, J.H. et al. (1987) Cell 50:129.
- Spicer, E.K. (1987) Proc. Natl. Acad. Sci. USA 84:5148.
Alternate Names
CD142, F3, Thromboplastin, Tissue Factor
Gene Symbol
F3
UniProt
Additional Coagulation Factor III/Tissue Factor Products
Product Documents for Human Coagulation Factor III/Tissue Factor Antibody [Biotin]
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Coagulation Factor III/Tissue Factor Antibody [Biotin]
For research use only
Related Research Areas
Citations for Human Coagulation Factor III/Tissue Factor Antibody [Biotin]
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Application: ELISASample Tested: Serum and PlasmaSpecies: HumanVerified Customer | Posted 07/06/2021we used this antibody for an ELISA to quantify human serum tissue factor H, works very well.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
