Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Flow Cytometry, Immunoprecipitation, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry, ELISA Capture, ELISA Development, Electron Microscopy

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Coagulation Factor III/Tissue Factor Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Coagulation Factor III/Tissue Factor
Gly34-Glu251
Accession # P13726

Specificity

Detects human Coagulation Factor III/Tissue Factor in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant mouse Coagulation Factor III/Tissue Factor is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Coagulation Factor III/
Tissue Factor Antibody

Detection of Human Coagulation Factor III/Tissue Factor by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line and A549 human lung carcinoma cell line (negative control). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Coagulation Factor III/Tissue Factor Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2339) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). A specific band was detected for Coagulation Factor III/Tissue Factor at approximately 48 kDa (as indicated). GAPDH (AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Applications for Human Coagulation Factor III/Tissue Factor Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: U937 human histiocytic lymphoma cell line treated with LPS

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cerebellum)

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Coagulation Factor III/Tissue Factor (Catalog # 2339-PA), see our available Western blot detection antibodies

Western Blot

0.5 µg/mL
Sample: A431 human epithelial carcinoma cell line

Reviewed Applications

Read 3 reviews rated 4.3 using AF2339 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Coagulation Factor III/Tissue Factor

Coagulation Factor III/Tissue Factor (TF), also known as thromboplastin and CD142, is an integral membrane protein found in a variety of cell types. It functions as a protein cofactor/receptor of Coagulation Factor VII, which is synthesized in the liver and circulated in the plasma (1). Upon binding of TF, the inactive factor VII is rapidly converted into activated VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and has also important coagulation-independent functions such as angiognesis (2). Synthesized as a 295 amino acid precursor, TF consists of a signal peptide (residues 1-32) and the mature chain (residues 33-295). As a type I membrane protein, it contains a transmembrane region (residues 252-274) and a cytoplasmic tail (residues 275-295) (3-6).

References

  1. Morrissey, J.H. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (ed) San Diego, Academic Press, p. 1659. 
  2. Versteeg, H.H. et al. (2003) Carcinogenesis 24:1009. 
  3. Scarpati, E.M. et al. (1987) Biochemistry 26:5234.
  4. Fisher, K.L. et al. (1987) Thromb. Res. 48:89.
  5. Morrissey, J.H. et al. (1987) Cell 50:129.
  6. Spicer, E.K. (1987) Proc. Natl. Acad. Sci. USA 84:5148.

Alternate Names

CD142, F3, Thromboplastin, Tissue Factor

Entrez Gene IDs

2152 (Human); 14066 (Mouse)

Gene Symbol

F3

UniProt

P13726

Additional Coagulation Factor III/Tissue Factor Products

Product Documents for Human Coagulation Factor III/Tissue Factor Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Coagulation Factor III/Tissue Factor Antibody

For research use only

Citations for Human Coagulation Factor III/
Tissue Factor Antibody

Customer Reviews (3)

4.3 out of 5
3 Customer Ratings
5 Stars
67%
4 Stars
0%
3 Stars
33%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Human Coagulation Factor III/Tissue Factor Antibody
    Name: Zoia Levashova
    Application: MSD assay
    Sample Tested: EDTA Plasma, Citrate Plasma and Serum
    Species: Human and Cynomolgus Monkey
    Verified Customer | Posted 05/30/2018
    Used as detection antibody after labeling with Sulfo-Tag according to manufacturer's protocol (Meso Scale Diagnostics LLC). Detection range – 1.5-25,000 pg/ml
    Human Coagulation Factor III/Tissue Factor Antibody AF2339
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 22828416
    Species: Human
    Verified Customer | Posted 01/06/2015
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 23468018
    Species: Human
    Verified Customer | Posted 01/06/2015

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Protocols

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