Detection of Human Coagulation Factor III by Western Blot.
Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line treated (+) with TNF-alpha. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Coagulation Factor III/Tissue Factor Monoclonal Antibody (Catalog # MAB2339) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Coagulation Factor III at approximately 50 and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Coagulation Factor III/Tissue Factor
Coagulation Factor III/Tissue Factor (TF), also known as thromboplastin and CD142, is a type I transmembrane protein found in a variety of cell types. It functions as a protein cofactor/receptor of Coagulation Factor VII, which is synthesized in the liver and circulated in the plasma (1). Upon binding of TF, the inactive factor VII is rapidly converted into activated VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and has also important coagulation-independent functions such as angiogenesis (2). TF is synthesized as a 295 amino acid (aa) precursor, with a signal peptide (aa 1-32), an extracellular domain (aa 33-251), a transmembrane region (aa 252-274) and a cytoplasmic tail (aa 275-295) (3‑6).
Morrissey, J.H. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (ed) San Diego, Academic Press, p. 1659.
Versteeg, H.H. et al. (2003) Carcinogenesis 24:1009.
Scarpati, E.M. et al. (1987) Biochemistry 26:5234.
Fisher, K.L. et al. (1987) Thromb. Res. 48:89.
Morrissey, J.H. et al. (1987) Cell 50:129.
Spicer, E.K. (1987) Proc. Natl. Acad. Sci. USA 84:5148.
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