Human Coagulation Factor III/Tissue Factor Antibody

(2 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human coagulation factor III/Tissue Factor in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse Tissue Factor is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 323514
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Coagulation Factor III
    Gly34-Glu251
    Accession # P13726
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Immunoprecipitation
    25 µg/mL
    Conditioned cell culture medium spiked with Recombinant Human Coagulation Factor III/Tissue Factor (Catalog # 2339-PA), see our available Western blot detection antibodies
    • Human Coagulation Factor III/Tissue Factor Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human Coagulation Factor III/Tissue Factor Antibody (Catalog # MAB2339)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human Coagulation Factor III/Tissue Factor Biotinylated Antibody (Catalog # BAF2339)
  • ELISA Standard
     
    Recombinant Human Coagulation Factor III/Tissue Factor, CF (Catalog # 2339-PA)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Coagulation Factor III by Western Blot.
Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line treated (+) with TNF-alpha. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Coagulation Factor III/Tissue Factor Monoclonal Antibody (Catalog # MAB2339) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Coagulation Factor III at approximately 50 and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Coagulation Factor III/Tissue Factor

Coagulation Factor III/Tissue Factor (TF), also known as thromboplastin and CD142, is a type I transmembrane protein found in a variety of cell types. It functions as a protein cofactor/receptor of Coagulation Factor VII, which is synthesized in the liver and circulated in the plasma (1). Upon binding of TF, the inactive factor VII is rapidly converted into activated VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and has also important coagulation-independent functions such as angiogenesis (2). TF is synthesized as a 295 amino acid (aa) precursor,  with a signal peptide (aa 1-32), an extracellular domain (aa 33-251), a transmembrane region (aa 252-274) and a cytoplasmic tail (aa 275-295) (3‑6).

  • References:
    1. Morrissey, J.H. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (ed) San Diego, Academic Press, p. 1659. 
    2. Versteeg, H.H. et al. (2003) Carcinogenesis 24:1009. 
    3. Scarpati, E.M. et al. (1987) Biochemistry 26:5234.
    4. Fisher, K.L. et al. (1987) Thromb. Res. 48:89.
    5. Morrissey, J.H. et al. (1987) Cell 50:129.
    6. Spicer, E.K. (1987) Proc. Natl. Acad. Sci. USA 84:5148.
  • Entrez Gene IDs:
    2152 (Human); 14066 (Mouse)
  • Alternate Names:
    CD142 antigen; CD142; coagulation factor III (thromboplastin, tissue factor); Coagulation Factor III; F3; FLJ17960; TF; TFA; Thromboplastin; Tissue Factor
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Applications
Sample Type
  1. Performance characteristics of combinations of host biomarkers to identify women with occult placental malaria: a case-control study from Malawi.
    Authors: Conroy AL, Liles WC, Molyneux ME
    PLoS ONE, 2011;6(12):e28540.
    Species: Human
    Sample Type: Plasma
    Application: ELISA Development
  2. Increased expression of inflammation-related co-stimulatory molecules by HUVECs from newborns with a strong family history of myocardial infarction stimulated with TNF-alpha and oxLDL.
    Authors: Mendez-Cruz AR, Paez A, Jimenez-Flores R, Reyes-Reali J, Varela E, Cerbulo-Vazquez A, Rodriguez E, Lopez-Marure R, Masso FA, Flores-Romo L, Montano LF
    Immunol. Lett., 2007;111(2):116-23.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
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