Human Coagulation Factor III/Tissue Factor Antibody

Catalog # Availability Size / Price Qty
MAB2339-100
MAB2339-500
MAB2339-SP
Human Coagulation Factor III/Tissue Factor Antibody in Data
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Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (1)

Human Coagulation Factor III/Tissue Factor Antibody Summary

Species Reactivity
Human
Specificity
Detects human coagulation factor III/Tissue Factor in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse Tissue Factor is observed.
Source
Monoclonal Mouse IgG2A Clone # 323514
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Coagulation Factor III
Gly34-Glu251
Accession # P13726
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Coagulation Factor III/Tissue Factor (Catalog # 2339-PA), see our available Western blot detection antibodies

Human Coagulation Factor III/Tissue Factor Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human Coagulation Factor III/Tissue Factor Biotinylated Antibody (Catalog # BAF2339)

Standard: Recombinant Human Coagulation Factor III/Tissue Factor, CF (Catalog # 2339-PA)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Coagulation Factor III by Western Blot. View Larger

Detection of Human Coagulation Factor III by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line treated (+) with TNF-a. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Coagulation Factor III/Tissue Factor Monoclonal Antibody (Catalog # MAB2339) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Coagulation Factor III at approximately 50 and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Coagulation Factor III/Tissue Factor

Coagulation Factor III/Tissue Factor (TF), also known as thromboplastin and CD142, is a type I transmembrane protein found in a variety of cell types. It functions as a protein cofactor/receptor of Coagulation Factor VII, which is synthesized in the liver and circulated in the plasma (1). Upon binding of TF, the inactive factor VII is rapidly converted into activated VIIa. The resulting 1:1 complex of VIIa and TF initiates the coagulation pathway and has also important coagulation-independent functions such as angiogenesis (2). TF is synthesized as a 295 amino acid (aa) precursor,  with a signal peptide (aa 1-32), an extracellular domain (aa 33-251), a transmembrane region (aa 252-274) and a cytoplasmic tail (aa 275-295) (3‑6).

References
  1. Morrissey, J.H. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (ed) San Diego, Academic Press, p. 1659. 
  2. Versteeg, H.H. et al. (2003) Carcinogenesis 24:1009. 
  3. Scarpati, E.M. et al. (1987) Biochemistry 26:5234.
  4. Fisher, K.L. et al. (1987) Thromb. Res. 48:89.
  5. Morrissey, J.H. et al. (1987) Cell 50:129.
  6. Spicer, E.K. (1987) Proc. Natl. Acad. Sci. USA 84:5148.
Entrez Gene IDs
2152 (Human); 14066 (Mouse)
Alternate Names
CD142 antigen; CD142; coagulation factor III (thromboplastin, tissue factor); Coagulation Factor III; F3; FLJ17960; TF; TFA; Thromboplastin; Tissue Factor

Product Datasheets

Citations for Human Coagulation Factor III/Tissue Factor Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Performance characteristics of combinations of host biomarkers to identify women with occult placental malaria: a case-control study from Malawi.
    Authors: Conroy AL, Liles WC, Molyneux ME
    PLoS ONE, 2011;6(12):e28540.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  2. Increased expression of inflammation-related co-stimulatory molecules by HUVECs from newborns with a strong family history of myocardial infarction stimulated with TNF-alpha and oxLDL.
    Authors: Mendez-Cruz AR, Paez A, Jimenez-Flores R, Reyes-Reali J, Varela E, Cerbulo-Vazquez A, Rodriguez E, Lopez-Marure R, Masso FA, Flores-Romo L, Montano LF
    Immunol. Lett., 2007;111(2):116-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

FAQs

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Coagulation Factor III/Tissue Factor Antibody

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Human Coagulation Factor III/Tissue Factor Antibody
By Anonymous on 06/22/2018
Application: MSD (Meso Scale Discovery) assay Sample Tested: EDTA Plasma Species: Human

After biotinylation, used as a capture reagent according to the manufacturer’s protocol (Meso Scale Diagnostics LLC). Dynamic range for human TF was 10-25,000 pg/mL.