Human Collagen XIII  alpha 1 Antibody

Catalog # Availability Size / Price Qty
AF6346
AF6346-SP
Human Collagen XIII  alpha 1 Antibody in Western Blot
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Citations (1)
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Human Collagen XIII  alpha 1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Collagen XIII in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse COL13A1 and less than 1% cross-reactivity with recombinant human (rh) COL23A1 and rhCOL25A1 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Collagen XIII
Glu109-Leu668
Accession # NP_542992
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Collagen XIII  alpha 1 by Western Blot. View Larger

Detection of Human Collagen XIII alpha 1 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF Membrane was probed with 1 µg/mL of Human Collagen XIII alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6346) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Two specific bands were detected for Collagen XIII a1 at approximately 100 and 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Collagen XIII alpha 1

COL13A1 (collagen 13-alpha 1) is a 95 kDa member of the transmembrane group of the collagen family of proteins. It is a type II transmembrane glycoprotein that is expressed by multiple cell types, including fibroblasts, endothelial cells, and cardiac muscle. COL13A1 forms disulfide-linked homotrimers and participates in cell adhesion by binding to the integrin alpha 1 subunit, nidogen-2, and fibronectin. Human COL13A1 is 717 amino acids (aa) in length (SwissProt #:Q5TAT6). It has an N‑terminal 44 amino acid cytoplasmic region plus a 656 amino acid extracellular domain (ECD) (aa 62-717). The ECD contains four non-collagenous (NC) regions (aa 1‑121, 217-269, 442-463, and 700-717) interspersed among three collagenous (COL) domains (aa 122-216, 270-441, and 464-699). Multiple splice forms exist and typically involve deletions of 12-30 aa between aa 220-705. Proteolytic cleavage after Arg108 generates an 85-90 kDa soluble form. Over aa 109-668 (based on a human isoform [NP_542992} that shows a deletion of aa 239-260, 551-565, and 616-627), human COL13A1 shares 91% aa sequence identity with mouse COL13A1.

Entrez Gene IDs
1305 (Human); 12817 (Mouse); 499431 (Rat)
Alternate Names
COL13A1; collagen alpha-1(XIII) chain; Collagen XIII alpha 1; collagen, type XIII, alpha 1; FLJ42485

Product Datasheets

Citation for Human Collagen XIII  alpha 1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Membrane associated collagen XIII promotes cancer metastasis and enhances anoikis resistance
    Authors: H Zhang, T Fredericks, G Xiong, Y Qi, PG Rychahou, JD Li, T Pihlajanie, W Xu, R Xu
    Breast Cancer Res., 2018;20(1):116.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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