Human Collagen XIII alpha 1 Antibody Summary
Accession # NP_542992
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Collagen XIII alpha 1 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF Membrane was probed with 1 µg/mL of Human Collagen XIII a1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6346) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Two specific bands were detected for Collagen XIII a1 at approximately 100 and 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Collagen XIII alpha 1
COL13A1 (collagen 13-alpha 1) is a 95 kDa member of the transmembrane group of the collagen family of proteins. It is a type II transmembrane glycoprotein that is expressed by multiple cell types, including fibroblasts, endothelial cells, and cardiac muscle. COL13A1 forms disulfide-linked homotrimers and participates in cell adhesion by binding to the integrin alpha 1 subunit, nidogen-2, and fibronectin. Human COL13A1 is 717 amino acids (aa) in length (SwissProt #:Q5TAT6). It has an N‑terminal 44 amino acid cytoplasmic region plus a 656 amino acid extracellular domain (ECD) (aa 62-717). The ECD contains four non-collagenous (NC) regions (aa 1‑121, 217-269, 442-463, and 700-717) interspersed among three collagenous (COL) domains (aa 122-216, 270-441, and 464-699). Multiple splice forms exist and typically involve deletions of 12-30 aa between aa 220-705. Proteolytic cleavage after Arg108 generates an 85-90 kDa soluble form. Over aa 109-668 (based on a human isoform [NP_542992} that shows a deletion of aa 239-260, 551-565, and 616-627), human COL13A1 shares 91% aa sequence identity with mouse COL13A1.
Citation for Human Collagen XIII alpha 1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Membrane associated collagen XIII promotes cancer metastasis and enhances anoikis resistance
Authors: H Zhang, T Fredericks, G Xiong, Y Qi, PG Rychahou, JD Li, T Pihlajanie, W Xu, R Xu
Breast Cancer Res., 2018;20(1):116.
Sample Types: Cell Lysates
Applications: Western Blot
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