Human Collagen XXV  alpha 1 Antibody

Catalog # Availability Size / Price Qty
AF4317
AF4317-SP
Human Collagen XXV  alpha 1 Antibody in Western Blot
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Human Collagen XXV  alpha 1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Collagen XXV alpha 1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant human COL23A1 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Collagen XXV alpha 1
Gly189-Lys654
Accession # NP_942014
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Collagen XXV  alpha 1 by Western Blot. View Larger

Detection of Human Collagen XXV  alpha 1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Collagen XXV  alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4317) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Collagen XXV  alpha 1 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Collagen XXV alpha 1

Collagen XXV (Collagen 25 alpha 1 chain; also CLAC-P) is a neuronal, 65‑75 kDa member of the Collagenous transmembrane (TM) subfamily of the Collagen protein family. It is synthesized as a 654 amino acid (aa) type II TM glycoprotein that contains a 33 aa cytoplasmic region (aa 1‑33) and a 600 aa extracellular domain (ECD) (aa 55‑654). The ECD contains seven Collagen-like domains. Furin cleavage between Arg112‑Glu113 generates a 70 kDa soluble fragment that homotrimerizes and binds to early A beta amyloid deposits. There are at least five multiple splice forms that impact the ECD. Amino acids 139‑146 and the seventh Collagen-like domain (aa 571‑630) are most often affected. Over aa 189‑654, human COL25A1 is 95% aa identical to mouse COL25A1.

Entrez Gene IDs
84570 (Human); 77018 (Mouse)
Alternate Names
Alzheimer disease amyloid-associated protein; AMY; CLAC; CLACP; CLAC-P; COL25A1; Collagen 25; collagen alpha-1(XXV) chain; Collagen XXV alpha 1; collagen, type XXV, alpha 1; Collagen-25; collagen-like Alzheimer amyloid plaque component; collagenous Alzheimer amyloid plaque component

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