Detects human Complement Component C5/C5a in ELISAs and Western blots. In sandwich immunoassays, detects human Complement Component C5a by itself or in the context of Complement Component C5. In Western blots, this antibody does not cross-react with recombinant human Complement Component C2, recombinant mouse (rm) Complement Component C3d, or rmComplement Component C5a.
Monoclonal Mouse IgG1 Clone # 295003
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human Complement Component C5a Thr678-Arg751 Accession # P01031
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Recombinant Human Complement Component C5a (Catalog # 2037-C5) under non-reducing conditions only
Human Complement Component C5/C5a Sandwich Immunoassay
ELISA Capture (Matched Antibody Pair)
Human Complement Component C5/C5a Antibody (Catalog # MAB2037)
ELISA Detection (Matched Antibody Pair)
Human Complement Component C5/C5a Biotinylated Antibody (Catalog # BAM20371)
Recombinant Human Complement Component C5a Protein (Catalog # 2037-C5)
Measured by its ability to neutralize Complement Component C5a-induced N-acetyl-beta -D-glucosaminidase release in the dibutyryl cyclic-AMP differentiated U937 human histiocytic lymphoma cell line. Klos, A. et al. (1992) Biochemistry 31:11274. The Neutralization Dose (ND50) is typically 0.04-0.12 µg/mL in the presence of 10 ng/mL Recombinant Human Complement Component C5a and cytochalasin‑B.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
N-Acetyl-beta -D-Glucosaminidase Release Induced by Complement Component C5a and Neutralization by Human Complement Component C5a Antibody.
Recombinant Human Complement Component C5a (Catalog # 2037-C5) induces N‑acetyl‑ beta ‑D‑glucosaminidase release in the the dibutyryl cyclic-AMP differentiated U937 human histiocytic lymphoma cell line in a dose-dependent manner (orange line). N-Acetyl-beta -D-Glucosaminidase Release elicited by Recombinant Human Complement Component C5a (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Complement Component C5a Monoclonal Antibody (Catalog # MAB2037). The ND50 is typically 0.04‑0.12 µg/mL in the presence of cytochalasin‑B.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Complement Component C5/C5a
Human complement component C5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as anaphylatoxins. C5a is a 74 amino acid (aa) peptide that is created by the activity of C5a convertase on the C5 alpha -chain (1, 2). Human C5a has four alpha -helices plus three intrachain disulfide bonds that create a triple loop structure (3). In serum, proteolytic processing removes the C-terminal arginine, creating a low activity C5a desArg74 molecule (1). Human C5a is 60% and 54% aa identical to mouse and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (C5aR/CD88) and a non-signaling GPCR termed C5L2 (4). Activation of Cd88 results in neutrophil chemotaxis and endothelial cell activation (1, 5). It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells.
Gerard, C. and N.P. Gerard (1994) Annu. Rev. Immunol. 12:775.
DiScipio, R.G. et al. (1983) J. Biol. Chem. 258:10629.
Huber-Lang, M.S. et al. (2003) J. Immunol. 170:6115.
Okinaga, S. et al. (2003) Biochemistry 42:9406.
Gerard, N.P. and C. Gerard (2002) Curr. Opin. Immunol. 14:705.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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