Human Complement Component C5/C5a Antibody Summary
Accession # P01031
under non-reducing conditions only
Human Complement Component C5/C5a Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
N-Acetyl-beta -D-Glucosamini-dase Release Induced by Complement Component C5a and Neutralization by Human Complement Component C5a Antibody. Recombinant Human Complement Component C5a (Catalog # 2037-C5) induces N-acetyl-beta -D-glucosaminidase release in the the dibutyryl cyclic-AMP differentiated U937 human histiocytic lymphoma cell line in a dose-dependent manner (orange line). N-Acetyl-beta -D-Glucosamin-idase Release elicited by Recombinant Human Complement Component C5a (10 ng/mL) is neutralized (green line) by increasing concen-trations of Mouse Anti-Human Complement Component C5a Monoclonal Antibody (Catalog # MAB2037). The ND50is typically 0.04-0.12 µg/mL in the presence of cytochalasin-B.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Complement Component C5/C5a
Human complement component C5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as anaphylatoxins. C5a is a 74 amino acid (aa) peptide that is created by the activity of C5a convertase on the C5 alpha -chain (1, 2). Human C5a has four alpha -helices plus three intrachain disulfide bonds that create a triple loop structure (3). In serum, proteolytic processing removes the C-terminal arginine, creating a low activity C5a desArg74 molecule (1). Human C5a is 60% and 54% aa identical to mouse and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (C5aR/CD88) and a non-signaling GPCR termed C5L2 (4). Activation of Cd88 results in neutrophil chemotaxis and endothelial cell activation (1, 5). It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells.
- Gerard, C. and N.P. Gerard (1994) Annu. Rev. Immunol. 12:775.
- DiScipio, R.G. et al. (1983) J. Biol. Chem. 258:10629.
- Huber-Lang, M.S. et al. (2003) J. Immunol. 170:6115.
- Okinaga, S. et al. (2003) Biochemistry 42:9406.
- Gerard, N.P. and C. Gerard (2002) Curr. Opin. Immunol. 14:705.
Citations for Human Complement Component C5/C5a Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
Filter your results:
Attenuation of Staphylococcus aureus-Induced Bacteremia by Human Mini-Antibodies Targeting the Complement Inhibitory Protein Efb.
Authors: Georgoutsou-Spyridonos M, Ricklin D, Pratsinis H, Perivolioti E, Pirmettis I, Garcia B, Geisbrecht B, Foukas P, Lambris J, Mastellos D, Sfyroera G
J Immunol, 2015;195(8):3946-58.
Sample Types: Plasma
Applications: ELISA Development (Capture)
Generation of multiple fluid-phase C3b:plasma protein complexes during complement activation: possible implications in C3 glomerulopathies.
Authors: Ramadass, Mahalaks, Ghebrehiwet, Berhane, Smith, Richard, Kew, Richard
J Immunol, 2014;192(3):1220-30.
Sample Types: Serum
Applications: ELISA Development (Capture)
C5a and TNF-alpha up-regulate the expression of tissue factor in intra-alveolar neutrophils of patients with the acute respiratory distress syndrome.
Authors: Kambas K, Markiewski MM, Pneumatikos IA, Rafail SS, Theodorou V, Konstantonis D, Kourtzelis I, Doumas MN, Magotti P, Deangelis RA, Lambris JD, Ritis KD
J. Immunol., 2008;180(11):7368-75.
Sample Types: BALF
Applications: ELISA Development
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human Complement Component C5/C5a Antibody
There are currently no reviews for this product. Be the first to review Human Complement Component C5/C5a Antibody and earn rewards!
Have you used Human Complement Component C5/C5a Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image