Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair)

Cited:

Luminex Development

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all Complement Factor D/Adipsin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Complement Factor D (R&D Systems, Catalog # 1824-SE)
Ile26-Ala253
Accession # P00746

Specificity

Detects human Complement Factor D in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross‑reactivity with rhFactor II, rhFactor X, and rhFactor XI is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Complement Factor D/Adipsin Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Complement Factor D/Adipsin (Catalog # 1824-SE)

Human Complement Factor D/Adipsin Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human Complement Factor D/Adipsin Antibody (Catalog # MAB18241)
  • Standard: Recombinant Human Complement Factor D Protein, CF (Catalog # 1824-SE)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4.5 using BAF1824 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Complement Factor D/Adipsin

Complement Factor D is a serine protease that catalyzes the initial proteolytic step in the alternative pathway of complement. Expressed in adipose tissue at high levels, factor D is also known as adipsin (1). It is an exceptionally specific protease and the only known protein substrate is factor B in complex with C3 (2). Factor D protease activity is regulated by reversible conformational changes, which differs from the majority of serine proteases whose regulation involves either activation by processing of the zymogens or inactivation by binding of the inhibitors. Compared to its physiologically important proteolytic activity, factor D has much lower activity toward synthetic peptide substrates. However, thioester substrates have been routinely used for assessing factor D activity (3).

References

  1. White, R.T. et al. (1992) J. Biol. Chem. 267:9210.
  2. Taylor, F.R. et al. (1999) Biochemistry 38:2849.
  3. Kim, S. et al. (1995) J. Biol. Chem. 270:24399.

Alternate Names

Adipsin, ADN, AMBP-1, CFD, PFD

Entrez Gene IDs

1675 (Human); 11537 (Mouse); 54249 (Rat)

Gene Symbol

CFD

UniProt

P00746

Additional Complement Factor D/Adipsin Products

Product Documents for Human Complement Factor D/Adipsin Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Complement Factor D/Adipsin Biotinylated Antibody

For research use only

Related Research Areas

Complement Pathway

Citations for Human Complement Factor D/Adipsin Biotinylated Antibody

Customer Reviews for Human Complement Factor D/Adipsin Biotinylated Antibody (2)

4.5 out of 5
2 Customer Ratings
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  • Human Complement Factor D/Adipsin Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Recombinant protein and Normal Human Serum
    Species: Human
    Verified Customer | Posted 01/10/2017
    Human Complement Factor D/Adipsin Biotinylated Antibody BAF1824
  • Human Complement Factor D/Adipsin Biotinylated Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HEK293 human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 06/08/2016

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