Human Complement Factor D/Adipsin Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF1824
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (2)

Human Complement Factor D/Adipsin Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Complement Factor D in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross‑reactivity with rhFactor II, rhFactor X, and rhFactor XI is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Complement Factor D (R&D Systems, Catalog # 1824-SE)
Ile26-Ala253
Accession # P00746
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Complement Factor D/Adipsin (Catalog # 1824-SE)

Human Complement Factor D/Adipsin Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human Complement Factor D/Adipsin Antibody (Catalog # MAB18241)

Standard: Recombinant Human Complement Factor D Protein, CF (Catalog # 1824-SE)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Complement Factor D/Adipsin

Complement Factor D is a serine protease that catalyzes the initial proteolytic step in the alternative pathway of complement. Expressed in adipose tissue at high levels, factor D is also known as adipsin (1). It is an exceptionally specific protease and the only known protein substrate is factor B in complex with C3 (2). Factor D protease activity is regulated by reversible conformational changes, which differs from the majority of serine proteases whose regulation involves either activation by processing of the zymogens or inactivation by binding of the inhibitors. Compared to its physiologically important proteolytic activity, factor D has much lower activity toward synthetic peptide substrates. However, thioester substrates have been routinely used for assessing factor D activity (3).

References
  1. White, R.T. et al. (1992) J. Biol. Chem. 267:9210.
  2. Taylor, F.R. et al. (1999) Biochemistry 38:2849.
  3. Kim, S. et al. (1995) J. Biol. Chem. 270:24399.
Entrez Gene IDs
1675 (Human); 11537 (Mouse); 54249 (Rat)
Alternate Names
Adipsin; ADN; ADNcomplement factor D; AMBP-1; C3 convertase activator; CFD; complement factor D (adipsin); complement factor D preproprotein; Complement Factor D; D component of complement (adipsin); DF; EC 3.4.21; EC 3.4.21.46; PFD; Properdin factor DADIPSIN

Product Datasheets

You must select a language.

x

Citation for Human Complement Factor D/Adipsin Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. A multiplex immunoassay for human adipokine profiling.
    Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
    Clin. Chem., 2010;56(0):1320.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human Complement Factor D/Adipsin Biotinylated Antibody

Average Rating: 4.5 (Based on 2 Reviews)

5 Star
50%
4 Star
50%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human Complement Factor D/Adipsin Biotinylated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human Complement Factor D/Adipsin Biotinylated Antibody
By Anonymous on 01/10/2017
Application: ELISA Sample Tested: Normal Human Serum,Recombinant protein Species: Human

Human Complement Factor D/Adipsin Biotinylated Antibody
By Anonymous on 06/08/2016
Application: WB Sample Tested: HEK293 human embryonic kidney cell line Species: Human