Complement Factor D is a serine protease that catalyzes the initial proteolytic step in the alternative pathway of complement. Expressed in adipose tissue at high levels, factor D is also known as adipsin (1). It is an exceptionally specific protease and the only known protein substrate is factor B in complex with C3 (2). Factor D protease activity is regulated by reversible conformational changes, which differs from the majority of serine proteases whose regulation involves either activation by processing of the zymogens or inactivation by binding of the inhibitors. Compared to its physiologically important proteolytic activity, factor D has much lower activity toward synthetic peptide substrates. However, thioester substrates have been routinely used for assessing factor D activity (3).
Human Complement Factor D/Adipsin Antibody (255719)
R&D Systems | Catalog # MAB18241
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Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Ile26-Ala253
Accession # P00746
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human Complement Factor D/Adipsin Antibody (255719)
Human Complement Factor D / Adipsin ELISA Standard Curve
Recombinant Human Complement Factor D/Adipsin (Catalog # 1824-SE) was serially diluted and captured by Mouse Anti-Human Complement Factor D/Adipsin Monoclonal Antibody (Catalog # MAB18241) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Complement Factor D/Adipsin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1824) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Human Complement Factor D/Adipsin Antibody (255719)
Human Complement Factor D/Adipsin Sandwich Immunoassay
Reviewed Applications
Read 1 review rated 5 using MAB18241 in the following applications:
Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Complement Factor D/Adipsin
References
- White, R.T. et al. (1992) J. Biol. Chem. 267:9210.
- Taylor, F.R. et al. (1999) Biochemistry 38:2849.
- Kim, S. et al. (1995) J. Biol. Chem. 270:24399.
Alternate Names
Gene Symbol
UniProt
Additional Complement Factor D/Adipsin Products
Product Documents for Human Complement Factor D/Adipsin Antibody (255719)
Certificate of Analysis
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Product Specific Notices for Human Complement Factor D/Adipsin Antibody (255719)
For research use only
Related Research Areas
Customer Reviews for Human Complement Factor D/Adipsin Antibody (255719) (1)
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Application: ELISASample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 05/12/2021Used this antibody to coat the plate in an adipsin immunoassay.
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