Complement Factor D is a serine protease that catalyzes the initial proteolytic step in the alternative pathway of complement. Expressed in adipose tissue at high levels, factor D is also known as adipsin (1). It is an exceptionally specific protease and the only known protein substrate is factor B in complex with C3 (2). Factor D protease activity is regulated by reversible conformational changes, which differs from the majority of serine proteases whose regulation involves either activation by processing of the zymogens or inactivation by binding of the inhibitors. Compared to its physiologically important proteolytic activity, factor D has much lower activity toward synthetic peptide substrates. However, thioester substrates have been routinely used for assessing factor D activity (3).
Key Product Details
Species Reactivity
Human
Applications
ELISA Capture (Matched Antibody Pair)
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 255719
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Complement Factor D
Ile26-Ala253
Accession # P00746
Ile26-Ala253
Accession # P00746
Specificity
Detects human Complement Factor D in ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Human Complement Factor D/Adipsin Antibody
Human Complement Factor D/Adipsin Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB18241 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Complement Factor D/Adipsin
References
- White, R.T. et al. (1992) J. Biol. Chem. 267:9210.
- Taylor, F.R. et al. (1999) Biochemistry 38:2849.
- Kim, S. et al. (1995) J. Biol. Chem. 270:24399.
Alternate Names
Adipsin, ADN, AMBP-1, CFD, PFD
Gene Symbol
CFD
UniProt
Additional Complement Factor D/Adipsin Products
Product Documents for Human Complement Factor D/Adipsin Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Complement Factor D/Adipsin Antibody
For research use only
Related Research Areas
Customer Reviews for Human Complement Factor D/Adipsin Antibody (1)
5 out of 5
1 Customer Rating
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Application: ELISASample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 05/12/2021Used this antibody to coat the plate in an adipsin immunoassay.
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