Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Complement Factor H Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Complement Factor H
Ser860-Arg1231
Accession # P08603

Specificity

Detects human Complement Factor H in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant mouse Complement Factor H is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Complement Factor H Antibody

Complement Factor H antibody in Human Liver by Immunohistochemistry (IHC-P).

Complement Factor H in Human Liver.

Complement Factor H was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human Complement Factor H Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4779) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Complement Factor H Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Western Blot

0.1 µg/mL
Sample: Recombinant Human Complement Factor H aa 860-1231 (Catalog # 4779-FH)

Reviewed Applications

Read 2 reviews rated 5 using AF4779 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Complement Factor H

Complement Factor H is a 155 kDa glycoprotein that provides critical negative regulation to the alternative pathway of complement cascade. It is secreted by Kupffer cells, hepatocytes, vascular endothelial cells, and platelets, and circulates in the serum at high concentration (1). Complement Factor H is composed of 20 SCRs (short consensus repeats), each of which consists of approximately 60 amino acids with four invariant Cys residues (2). Alternate splicing generates an isoform that is truncated following SCR7. Complement Factor H interacts with cell surface polyanions including heparin and sialoglycoproteins (3-6), and immobilized Complement Factor H supports the CD11b/CD18 integrin-dependent adhesion of neutrophils (7). It prevents local complement activation by sequestering complement component C3b, accelerating the decay of C3 and C5 convertases, and functions as a cofactor for the C3b inactivator, Factor I (1, 3, 6, 8). The recombinant protein expressed here corresponds to SCR15-20, which encompass the primary binding sites for heparin and C3b, as well as for the peptide hormone adrenomedullin (4, 9‑11). Within SCR15-20, human Complement Factor H shares 60% and 63% amino acid sequence identity with mouse and rat Complement Factor H, respectively. Dozens of mutations clustered in SCR15-20 are associated with atypical hemolytic uremic syndrome, a disorder characterized by anemia, thrombocytopenia, and renal failure (12). Binding of Complement Factor H to tumor cell-associated dentin matrix protein 1, bone sialoprotein, or osteopontin results in the protection of that cell from complement-mediated lysis (13, 14). A variety of pathogenic microbes also express Complement Factor H binding molecules that interfere with immune clearance of the infection (15).

References

  1. Schmidt, C.Q. et al. (2008) Clin. Exp. Immunol. 151:14. 
  2. Ripoche, J. et al. (1988) Biochem. J. 249:593. 
  3. Meri, S. and M.K. Pangburn (1990) Proc. Natl. Acad. Sci. USA 87:3982. 
  4. Jokiranta, T.S. et al. (2005) Am. J. Pathol. 167:1173. 
  5. Blackmore, T.K. et al. (1998) J. Immunol. 160:3342. 
  6. Hellwage, J. et al. (2002) J. Immunol. 169:6935. 
  7. DiScipio, R.G. et al. (1998) J. Immunol. 160:4057. 
  8. Sharma, A.K. and M.K. Pangburn (1996) Proc. Natl. Acad. Sci. USA 93:10996.
  9. Oppermann, M. et al. (2006) Clin. Exp. Immunol. 144:342.
  10. Pangburn, M.K. et al. (2000) J. Immunol. 164:4742.
  11. Martinez, A. et al. (2003) Hypertens. Res. 26:S55.
  12. de Cordoba, S.R. and E.G. de Jorge (2008) Clin. Exp. Immunol. 151:1.
  13. Jain, A. et al. (2002) J. Biol. Chem. 277:13700.
  14. Fedarko, N.S. et al. (2000) J. Biol. Chem. 275:16666.
  15. Kraiczy, P. and R. Wurzner (2006) Mol. Immunol. 43:31.

Alternate Names

ARMD4, ARMS1, beta-1-H-globulin, CFH, CFHL3, FH, FHL1, HF1, HF2, HUS

Entrez Gene IDs

3075 (Human); 12628 (Mouse); 155012 (Rat)

Gene Symbol

CFH

UniProt

P08603

Additional Complement Factor H Products

Product Documents for Human Complement Factor H Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Complement Factor H Antibody

For research use only

Citations for Human Complement Factor H Antibody

Customer Reviews for Human Complement Factor H Antibody (2)

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  • Human Complement Factor H Antibody
    Name: Anonymous
    Application: Simple Western
    Sample Tested: Human serum and Purified protein
    Species: Human
    Verified Customer | Posted 09/25/2019
    Input samples were dilution series of human serum pool or purified complement factor H. The AF4779 antibody was tested at three different concentrations; 10, 5 and 1 ug/ml (left to right). Samples were analysed by Simple Western under reducing conditions and the 12-230 kDa separation module.
    Human Complement Factor H Antibody AF4779
  • Human Complement Factor H Antibody
    Name: Anonymous
    Application: Simple Western
    Sample Tested: Serum and purified complement factor H
    Species: Human
    Verified Customer | Posted 09/06/2019
    Input samples were dilution series of human serum pool or purified complement factor H. The AF4779 antibody was tested at three different concentrations; 10, 5 and 1 ug/ml (left to right). Samples were analysed by Simple Western under reducing conditions and the 12-230 kDa separation module.
    Human Complement Factor H Antibody AF4779

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