Human Complement Factor H Antibody

Catalog # Availability Size / Price Qty
Complement Factor H in Human Liver.
1 Image
Product Details
Supplemental Products
Reviews (1)

Human Complement Factor H Antibody Summary

Species Reactivity
Detects human Complement Factor H in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant mouse Complement Factor H is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant human Complement Factor H
Accession # P08603
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human Complement Factor H aa 860-1231 (Catalog # 4779-FH)
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Complement Factor H antibody in Human Liver by Immunohistochemistry (IHC-P). View Larger

Complement Factor H in Human Liver. Complement Factor H was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human Complement Factor H Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4779) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Complement Factor H

Complement Factor H is a 155 kDa glycoprotein that provides critical negative regulation to the alternative pathway of complement cascade. It is secreted by Kupffer cells, hepatocytes, vascular endothelial cells, and platelets, and circulates in the serum at high concentration (1). Complement Factor H is composed of 20 SCRs (short consensus repeats), each of which consists of approximately 60 amino acids with four invariant Cys residues (2). Alternate splicing generates an isoform that is truncated following SCR7. Complement Factor H interacts with cell surface polyanions including heparin and sialoglycoproteins (3-6), and immobilized Complement Factor H supports the CD11b/CD18 integrin-dependent adhesion of neutrophils (7). It prevents local complement activation by sequestering complement component C3b, accelerating the decay of C3 and C5 convertases, and functions as a cofactor for the C3b inactivator, Factor I (1, 3, 6, 8). The recombinant protein expressed here corresponds to SCR15-20, which encompass the primary binding sites for heparin and C3b, as well as for the peptide hormone adrenomedullin (4, 9‑11). Within SCR15-20, human Complement Factor H shares 60% and 63% amino acid sequence identity with mouse and rat Complement Factor H, respectively. Dozens of mutations clustered in SCR15-20 are associated with atypical hemolytic uremic syndrome, a disorder characterized by anemia, thrombocytopenia, and renal failure (12). Binding of Complement Factor H to tumor cell-associated dentin matrix protein 1, bone sialoprotein, or osteopontin results in the protection of that cell from complement-mediated lysis (13, 14). A variety of pathogenic microbes also express Complement Factor H binding molecules that interfere with immune clearance of the infection (15).

  1. Schmidt, C.Q. et al. (2008) Clin. Exp. Immunol. 151:14. 
  2. Ripoche, J. et al. (1988) Biochem. J. 249:593. 
  3. Meri, S. and M.K. Pangburn (1990) Proc. Natl. Acad. Sci. USA 87:3982. 
  4. Jokiranta, T.S. et al. (2005) Am. J. Pathol. 167:1173. 
  5. Blackmore, T.K. et al. (1998) J. Immunol. 160:3342. 
  6. Hellwage, J. et al. (2002) J. Immunol. 169:6935. 
  7. DiScipio, R.G. et al. (1998) J. Immunol. 160:4057. 
  8. Sharma, A.K. and M.K. Pangburn (1996) Proc. Natl. Acad. Sci. USA 93:10996.
  9. Oppermann, M. et al. (2006) Clin. Exp. Immunol. 144:342.
  10. Pangburn, M.K. et al. (2000) J. Immunol. 164:4742.
  11. Martinez, A. et al. (2003) Hypertens. Res. 26:S55.
  12. de Cordoba, S.R. and E.G. de Jorge (2008) Clin. Exp. Immunol. 151:1.
  13. Jain, A. et al. (2002) J. Biol. Chem. 277:13700.
  14. Fedarko, N.S. et al. (2000) J. Biol. Chem. 275:16666.
  15. Kraiczy, P. and R. Wurzner (2006) Mol. Immunol. 43:31.
Entrez Gene IDs
3075 (Human); 12628 (Mouse); 155012 (Rat)
Alternate Names
adrenomedullin binding protein; age-related maculopathy susceptibility 1; AHUS1; AMBP1; ARMD4; ARMS1; beta-1H; beta-1-H-globulin ; beta-1-H-globulin; CFH; CFHL3; Complement Factor H; factor H; factor H-like 1; FH; FHL1; H factor 1 (complement); H factor 1; H factor 2 (complement); HF; HF1; HF1ARMS1; HF2; HUS; HUSMGC88246

Product Datasheets

You must select a language.



No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Complement Factor H Antibody

Average Rating: 5 (Based on 1 Review)

5 Star
4 Star
3 Star
2 Star
1 Star

Have you used Human Complement Factor H Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:

Human Complement Factor H Antibody
By Anonymous on 09/06/2019
Application: Simple Western Sample Tested: purified complement factor H,Serum Species: Human

Input samples were dilution series of human serum pool or purified complement factor H. The AF4779 antibody was tested at three different concentrations; 10, 5 and 1 ug/ml (left to right).
Samples were analysed by Simple Western under reducing conditions and the 12-230 kDa separation module.