Human Complement Factor H Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Complement Factor H in direct ELISAs and Western blots. In direct ELISAs and Western blots,  approximately 5% cross‑reactivity with recombinant mouse Complement Factor H is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Complement Factor H
    Ser860-Arg1231
    Accession # P08603
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human Complement Factor H aa 860-1231 (Catalog # 4779-FH)
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Complement Factor H in Human Liver. Complement Factor H was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human Complement Factor H Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4779) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Complement Factor H

Complement Factor H is a 155 kDa glycoprotein that provides critical negative regulation to the alternative pathway of complement cascade. It is secreted by Kupffer cells, hepatocytes, vascular endothelial cells, and platelets, and circulates in the serum at high concentration (1). Complement Factor H is composed of 20 SCRs (short consensus repeats), each of which consists of approximately 60 amino acids with four invariant Cys residues (2). Alternate splicing generates an isoform that is truncated following SCR7. Complement Factor H interacts with cell surface polyanions including heparin and sialoglycoproteins (3-6), and immobilized Complement Factor H supports the CD11b/CD18 integrin-dependent adhesion of neutrophils (7). It prevents local complement activation by sequestering complement component C3b, accelerating the decay of C3 and C5 convertases, and functions as a cofactor for the C3b inactivator, Factor I (1, 3, 6, 8). The recombinant protein expressed here corresponds to SCR15-20, which encompass the primary binding sites for heparin and C3b, as well as for the peptide hormone adrenomedullin (4, 9‑11). Within SCR15-20, human Complement Factor H shares 60% and 63% amino acid sequence identity with mouse and rat Complement Factor H, respectively. Dozens of mutations clustered in SCR15-20 are associated with atypical hemolytic uremic syndrome, a disorder characterized by anemia, thrombocytopenia, and renal failure (12). Binding of Complement Factor H to tumor cell-associated dentin matrix protein 1, bone sialoprotein, or osteopontin results in the protection of that cell from complement-mediated lysis (13, 14). A variety of pathogenic microbes also express Complement Factor H binding molecules that interfere with immune clearance of the infection (15).

  • References:
    1. Schmidt, C.Q. et al. (2008) Clin. Exp. Immunol. 151:14. 
    2. Ripoche, J. et al. (1988) Biochem. J. 249:593. 
    3. Meri, S. and M.K. Pangburn (1990) Proc. Natl. Acad. Sci. USA 87:3982. 
    4. Jokiranta, T.S. et al. (2005) Am. J. Pathol. 167:1173. 
    5. Blackmore, T.K. et al. (1998) J. Immunol. 160:3342. 
    6. Hellwage, J. et al. (2002) J. Immunol. 169:6935. 
    7. DiScipio, R.G. et al. (1998) J. Immunol. 160:4057. 
    8. Sharma, A.K. and M.K. Pangburn (1996) Proc. Natl. Acad. Sci. USA 93:10996.
    9. Oppermann, M. et al. (2006) Clin. Exp. Immunol. 144:342.
    10. Pangburn, M.K. et al. (2000) J. Immunol. 164:4742.
    11. Martinez, A. et al. (2003) Hypertens. Res. 26:S55.
    12. de Cordoba, S.R. and E.G. de Jorge (2008) Clin. Exp. Immunol. 151:1.
    13. Jain, A. et al. (2002) J. Biol. Chem. 277:13700.
    14. Fedarko, N.S. et al. (2000) J. Biol. Chem. 275:16666.
    15. Kraiczy, P. and R. Wurzner (2006) Mol. Immunol. 43:31.
  • Entrez Gene IDs:
    3075 (Human); 12628 (Mouse); 155012 (Rat)
  • Alternate Names:
    adrenomedullin binding protein; age-related maculopathy susceptibility 1; AHUS1; AMBP1; ARMD4; ARMS1; beta-1H; beta-1-H-globulin ; beta-1-H-globulin; CFH; CFHL3; Complement Factor H; factor H; factor H-like 1; FH; FHL1; H factor 1 (complement); H factor 1; H factor 2 (complement); HF; HF1; HF1ARMS1; HF2; HUS; HUSMGC88246
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