Detects human Complement Factor H-related 4/CFHR4 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human (rh) CFHR1, rhCFHR2, and rhCFHR5 is observed.
Polyclonal Sheep IgG
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Complement Factor H-related 4/CFHR4 Arg41-Glu331 (Ser79Leu) Accession # Q92496
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Complement Factor H-related 4/CFHR4 by Western Blot. Western blot shows lysates of human plasma. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Complement Factor H-related 4/CFHR4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5980) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Complement Factor H-related 4/CFHR4 at approximately 86 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Complement Factor H-related 4/CFHR4
FHR4 (Complement factor H-related protein 4; also FHR4B and CFHR4) is a 45 kDa glycoprotein member of the factor H-related protein family. It is synthesized by hepatocytes and circulates in plasma. FHR4(B) binds to C3b and C3d. It also binds to native (pentameric) C-reactive protein (pCRP), and is suggested to promote pCRP binding to necrotic cells surfaces, thus promoting cell clearance. Mature human CFHR is 312 amino acids (aa) in length. It contains five Sushi/SCR domains, the first of which (aa 23-85) is associated with pCRP binding. There is a circulating, 86 kDa alternative splice form termed FHR4A that shows an insertion of 247 aa after Asp92. This is considered the dominant isoform, and appears to show the same activity as CFHR. Over aa 41-331, human CFHR shares 61% aa identity with a related molecule found in rat.
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