Detection of Human COMT by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and human placenta tissue. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human COMT Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7386) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for COMT at approximately 25 and 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
COMT (Catechol-O-MethylTransferase) is a 29-30 kDa member of the COMT family of enzymes. It shares the same acronym with plant COMT, or caffeic acid 3-O-methyltransferase that is involved in phenylpropanoid synthesis. Mammalian COMT is widely expressed, and catalyzes the O-methylation of catecholamines, dopamine and estrogen. Human COMT is a 271 amino acid (aa) type II transmembrane (TM) protein. It contains a six aa cytoplasmic N-terminus, an 20 aa TM segment, and a 245 aa luminal/extracellular region that possesses a methyltransferase domain (aa 112-217). This TM form of COMT (termed MB-COMT) is generally associated with pre- and postsynaptic neurons plus glia, and is suggested to be very important in deactivating neurotransmitters, an activity that likely impacts cognition. There is also a 25-26 kDa COMT isoform (termed S-COMT) that utilizes an alternative start site at Met51. This isoform is cytoplasmic and generally found outside the CNS in cells such as mammary and intestinal epithelium, renal tubule epithelium, pancreatic beta -cells, monocytes and mast cells. S-COMT is believed to inactive toxic catechols (hydroxylated benzenes found in plant walls). MB-CORM exhibits high affinity:low capacity activity, while S-COMT shows low affinity:high capacity activity. Two additional alternative start sites have been reported. One starts at Met90, while a second possesses a start site 38 aa upstream of the MB-COMT start site. Over aa 52-271, human COMT shares 79% aa sequence identity with mouse COMT.
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.