Human COMT Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human COMT in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human COMT
    Gly52-Pro271 (Val158Met)
    Accession # P21964
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human COMT by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and human placenta tissue. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human COMT Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7386) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for COMT at approximately 25 and 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: COMT

COMT (Catechol-O-MethylTransferase) is a 29-30 kDa member of the COMT family of enzymes. It shares the same acronym with plant COMT, or caffeic acid 3-O-methyltransferase that is involved in phenylpropanoid synthesis. Mammalian COMT is widely expressed, and catalyzes the O-methylation of catecholamines, dopamine and estrogen. Human COMT is a 271 amino acid (aa) type II transmembrane (TM) protein. It contains a six aa cytoplasmic N-terminus, an 20 aa TM segment, and a 245 aa luminal/extracellular region that possesses a methyltransferase domain (aa 112-217). This TM form of COMT (termed MB-COMT) is generally associated with pre- and postsynaptic neurons plus glia, and is suggested to be very important in deactivating neurotransmitters, an activity that likely impacts cognition. There is also a 25-26 kDa COMT isoform (termed S-COMT) that utilizes an alternative start site at Met51. This isoform is cytoplasmic and generally found outside the CNS in cells such as mammary and intestinal epithelium, renal tubule epithelium, pancreatic beta -cells, monocytes and mast cells. S-COMT is believed to inactive toxic catechols (hydroxylated benzenes found in plant walls). MB-CORM exhibits high affinity:low capacity activity, while S-COMT shows low affinity:high capacity activity. Two additional alternative start sites have been reported. One starts at Met90, while a second possesses a start site 38 aa upstream of the MB-COMT start site. Over aa 52-271, human COMT shares 79% aa sequence identity with mouse COMT.

  • Long Name:
    Catechol O-Methyltransferase
  • Entrez Gene IDs:
    1312 (Human)
  • Alternate Names:
    catechol O-methyltransferase; catechol-O-methyltransferase; COMT; EC 2.1.1.6
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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