Human Cripto-1 Alexa Fluor® 488‑conjugated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Cripto-1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Cripto-1, recombinant human (rh) EGF, rhTGF‑ alpha, rhTGF-beta 1, rhTGF-beta 2, rhTGF-beta 3, or rhCryptic is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 89633
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Cripto-1
    Arg38-Tyr188
    Accession # P13385
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Cripto-1 in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Mouse Anti-Human Cripto-1 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB2772G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of Cripto-1 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human Cripto-1 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB2772G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Cripto

Cripto is the founding member of the epidermal growth factor-CriptoFRL1Cryptic (EGF-CFC) family of signaling proteins that function in cancer and various developmental processes. These developmental processes include: formation of the germ layers and dorsal organizer, specification of anterior-posterior and left-right axes, and differentiation of heart muscle (1, 2). Other members of the EGF-CFC family include Cryptic, Xenopus FRL-1 and zebrafish OEP (one-eyed pinhead). Overall sequence identity between members of the family is low, but they do share several common domains: a variant EGF-like motif, a novel conserved
cysteine-rich domain (called CFC domain), and a C-terminal hydrophobic region. Most EGF-CFC members have a glycosyl-phosphatidylinositol (GPI) anchoring site at the C-terminus and exist as extracellular membrane-anchored proteins. However, naturally-occurring soluble isoforms also exist. Human Cripto shares 66% and 28% amino acid identity with mouse Cripto and zebrafish OEP, respectively (2). Despite weak conservation in amino acid identity, EGF-CFC family members appear to function similarly in assays for phenotypic rescue of zebrafish oep mutants (2). Both secreted and membrane bound forms of Cripto demonstrate biological activity (3). Cripto, also known as CFC-2 or TDGF-1 (teratocarcinoma-derived growth factor), was originally isolated from an undifferentiated human teratocarcinoma cell line as a potential oncogene. It is overexpressed in many types of cancers and acts as a growth factor for tumors (4). Genetic evidence from mice and zebrafish points to a role for Cripto as an essential cofactor in Nodal signaling. Cripto and OEP mutants display defects in mesoderm induction and heart morphogenesis, similar to phenotypes seen in Nodal mutants (2).Cripto acts as a cofactor for Nodal by recruiting the Activin type I Receptor, ALK-4, leading to an
Act RIIB-ALK4-Cripto-Nodal complex for signaling (1, 3). Cripto also forms a complex with activin and Act RIIs to block activin signaling (5). Studies have shown that other TGF-beta superfamily members such as Vg1 and GDF-1 also require EGF-CFC cofactors (6). Cripto can also activate mitogen-activated protein kinase (MAPK) and Akt pathways independently of Nodal by directly binding to a membrane-associated heparan sulfate proteoglycan, glypican-1 (7).

  • References:
    1. Rosa, F.M. (2002) Science’s STKE http://stke.sciencemag.org/.
    2. Shen, M. and A. Schier (2000) Trends Genet. 16:303.
    3. Yan, Y-T. et al. (2002) Mol. Cell Biol. 22:4439.
    4. Salomon, D. et al. (2000) Endocrine-Rel. Cancer 7:199.
    5. Gray, P.C. et al. (2003) Proc. Natl. Acad. Sci. USA 100:5193.
    6. Cheng, S. et al. (2003) Genes & Dev. 17:31.
    7. Bianco, C. et al. (2003) Cancer Research 63:1192.
  • Long Name:
    Teratocarcinoma-derived Growth Factor/Cripto Growth Factor
  • Entrez Gene IDs:
    6997 (Human); 21667 (Mouse)
  • Alternate Names:
    CFC-2; CR; Cripto; Cripto-1 growth factor; Cripto-1; CRIPTOCRGF; Epidermal growth factor-like cripto protein CR1; TDGF1; TDGF-1; teratocarcinoma-derived growth factor 1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Alexa Fluor® 488-conjugated Antibody

Ctrl IC002G 3  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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