Human CRTAC1 Isoform 1 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human CRTAC1 Isoform 1 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human CRTAC1 Isoform 2 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human CRTAC1 Isoform 1
    Ser28-Cys661
    Accession # Q9NQ79
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human CRTAC1 by Western Blot. Western blot shows lysates of human cartilage tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CRTAC1 Isoform 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5234) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CRTAC1 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of Human CRTAC1 by Simple WesternTM. Simple Western lane view shows lysates of human cartilage tissue, loaded at 0.5 mg/mL. A specific band was detected for CRTAC1 at approximately 97 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human CRTAC1 Isoform 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5234) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CRTAC1

CRTAC1 (cartilage acidic protein 1; also CEP-68) is a 95‑105 kDa member of a novel family of EGF domain-containing proteins. It is secreted by articular chondrocytes and may play a role in either cartilage matrix organization, or cell-matrix adhesion. Mature human CRTAC1 is 634 amino acids (aa) in length. It contains four FG-GAP (PheGly-GlyAlaPro) domains (aa 46‑437) and one EGF-like motif (aa 559‑605). Multiple splice forms exist. There are two alternate start sites at Met9 and Met211 that may be accompanied by a 39 aa substitution for the C-terminal 55 aa, or an 84 aa substitution for aa 545‑661. Over aa 28‑661, human CRTAC1 shares 91% aa identity with mouse CRTAC1. This form in mouse, however, is more equivalent to the human isoform that shows a C-terminal 39 aa substitution. In this case, there is 95% aa identity between mouse and human.

  • Long Name:
    Cartilage Acidic Protein 1
  • Entrez Gene IDs:
    55118 (Human); 72832 (Mouse); 171438 (Rat)
  • Alternate Names:
    ASPIC; ASPIC1; ASPIC1CEP-68acidic secreted protein in cartilage; cartilage acidic protein 1; CEP68; chondrocyte expressed protein 68 kDa CEP-68,68 kDa chondrocyte-expressed protein; CRTAC1; FLJ10320
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