Human CRTAC1 Isoform 1 Antibody Summary
Accession # Q9NQ79
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human CRTAC1 by Western Blot. Western blot shows lysates of human cartilage tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CRTAC1 Isoform 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5234) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CRTAC1 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of Human CRTAC1 by Simple WesternTM. Simple Western lane view shows lysates of human cartilage tissue, loaded at 0.5 mg/mL. A specific band was detected for CRTAC1 at approximately 97 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human CRTAC1 Isoform 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5234) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
CRTAC1 (cartilage acidic protein 1; also CEP-68) is a 95‑105 kDa member of a novel family of EGF domain-containing proteins. It is secreted by articular chondrocytes and may play a role in either cartilage matrix organization, or cell-matrix adhesion. Mature human CRTAC1 is 634 amino acids (aa) in length. It contains four FG-GAP (PheGly-GlyAlaPro) domains (aa 46‑437) and one EGF-like motif (aa 559‑605). Multiple splice forms exist. There are two alternate start sites at Met9 and Met211 that may be accompanied by a 39 aa substitution for the C-terminal 55 aa, or an 84 aa substitution for aa 545‑661. Over aa 28‑661, human CRTAC1 shares 91% aa identity with mouse CRTAC1. This form in mouse, however, is more equivalent to the human isoform that shows a C-terminal 39 aa substitution. In this case, there is 95% aa identity between mouse and human.
Citation for Human CRTAC1 Isoform 1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Cerebrospinal fluid level of Nogo receptor 1 antagonist lateral olfactory tract usher substance (LOTUS) correlates inversely with the extent of neuroinflammation
Authors: K Takahashi, H Takeuchi, Y Kurihara, H Doi, M Kunii, K Tanaka, H Nakamura, R Fukai, A Tomita-Kat, M Tada, Y Higashiyam, H Joki, S Koyano, K Takei, F Tanaka
J Neuroinflammation, 2018;15(1):46.
Sample Types: CSF
Applications: Western Blot
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