Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all CRTAC1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CRTAC1 Isoform 1
Ser28-Cys661
Accession # Q9NQ79

Specificity

Detects human CRTAC1 Isoform 1 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human CRTAC1 Isoform 2 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human CRTAC1 Isoform 1 Antibody

Detection of Human CRTAC1 antibody by Western Blot.

Detection of Human CRTAC1 by Western Blot.

Western blot shows lysates of human cartilage tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CRTAC1 Isoform 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5234) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CRTAC1 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of Human CRTAC1 antibody by Simple WesternTM.

Detection of Human CRTAC1 by Simple WesternTM.

Simple Western lane view shows lysates of human cartilage tissue, loaded at 0.5 mg/mL. A specific band was detected for CRTAC1 at approximately 97 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human CRTAC1 Isoform 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5234) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human CRTAC1 Isoform 1 Antibody

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: Human cartilage tissue

Western Blot

1 µg/mL
Sample: Human cartilage tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CRTAC1

CRTAC1 (cartilage acidic protein 1; also CEP-68) is a 95‑105 kDa member of a novel family of EGF domain-containing proteins. It is secreted by articular chondrocytes and may play a role in either cartilage matrix organization, or cell-matrix adhesion. Mature human CRTAC1 is 634 amino acids (aa) in length. It contains four FG-GAP (PheGly-GlyAlaPro) domains (aa 46‑437) and one EGF-like motif (aa 559‑605). Multiple splice forms exist. There are two alternate start sites at Met9 and Met211 that may be accompanied by a 39 aa substitution for the C-terminal 55 aa, or an 84 aa substitution for aa 545‑661. Over aa 28‑661, human CRTAC1 shares 91% aa identity with mouse CRTAC1. This form in mouse, however, is more equivalent to the human isoform that shows a C-terminal 39 aa substitution. In this case, there is 95% aa identity between mouse and human.

Long Name

Cartilage Acidic Protein 1

Alternate Names

ASPIC1, CEP68

Entrez Gene IDs

55118 (Human); 72832 (Mouse); 171438 (Rat)

Gene Symbol

CRTAC1

UniProt

Q9NQ79

Additional CRTAC1 Products

Product Documents for Human CRTAC1 Isoform 1 Antibody

Certificate of Analysis

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Product Specific Notices for Human CRTAC1 Isoform 1 Antibody

For research use only

Citations for Human CRTAC1 Isoform 1 Antibody

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