Human CRTAC1 Isoform 1 Antibody Summary
Ser28-Cys661
Accession # Q9NQ79
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human CRTAC1 by Western Blot. Western blot shows lysates of human cartilage tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CRTAC1 Isoform 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5234) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CRTAC1 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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Detection of Human CRTAC1 by Simple WesternTM. Simple Western lane view shows lysates of human cartilage tissue, loaded at 0.5 mg/mL. A specific band was detected for CRTAC1 at approximately 97 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human CRTAC1 Isoform 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5234) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CRTAC1
CRTAC1 (cartilage acidic protein 1; also CEP-68) is a 95‑105 kDa member of a novel family of EGF domain-containing proteins. It is secreted by articular chondrocytes and may play a role in either cartilage matrix organization, or cell-matrix adhesion. Mature human CRTAC1 is 634 amino acids (aa) in length. It contains four FG-GAP (PheGly-GlyAlaPro) domains (aa 46‑437) and one EGF-like motif (aa 559‑605). Multiple splice forms exist. There are two alternate start sites at Met9 and Met211 that may be accompanied by a 39 aa substitution for the C-terminal 55 aa, or an 84 aa substitution for aa 545‑661. Over aa 28‑661, human CRTAC1 shares 91% aa identity with mouse CRTAC1. This form in mouse, however, is more equivalent to the human isoform that shows a C-terminal 39 aa substitution. In this case, there is 95% aa identity between mouse and human.
Product Datasheets
Citation for Human CRTAC1 Isoform 1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Cerebrospinal fluid level of Nogo receptor 1 antagonist lateral olfactory tract usher substance (LOTUS) correlates inversely with the extent of neuroinflammation
Authors: K Takahashi, H Takeuchi, Y Kurihara, H Doi, M Kunii, K Tanaka, H Nakamura, R Fukai, A Tomita-Kat, M Tada, Y Higashiyam, H Joki, S Koyano, K Takei, F Tanaka
J Neuroinflammation, 2018-02-17;15(1):46.
Species: Human, Mouse
Sample Types: CSF, Tissue Homogenates
Applications: Western Blot
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