Human CRTH-2 Alexa Fluor® 647-conjugated Antibody

    
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  • Species Reactivity
    Human
  • Specificity
    Detects human CRTH‑2 in ELISA.
  • Source
    Monoclonal Mouse IgG2a Clone # 301109
  • Immunogen
    NS0 mouse myeloma cell line transfected with human CRTH-2
    Met1-Ser395
    Accession # Q9Y5Y4
  • Formulation
    Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 647
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25-1 µg/106 cells
    Human peripheral blood lymphocytes
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CRTH-2
CRTH-2, also known as GPR44, is a 7TM G protein-coupled receptor that is expressed by activated Th2 cells and eosinophils. CRTH-2 binds Prostaglandin D2 and induces chemotaxis of these cells in allergic and inflammatory immune responses. Human and mouse CRTH-2 share 78% amino acid sequence identity.
  • Long Name:
    Chemoattractant Receptor-homologous Molecule Expressed on Th2 Cells
  • Entrez Gene IDs:
    11251 (Human); 14764 (Mouse); 309212 (Rat)
  • Alternate Names:
    CD294 antigen; CD294; Chemoattractant receptor-homologous molecule expressed on Th2 cells; CRTH2; CRTH-2; CRTH2chemoattractant receptor homologous molecule expressed on T helper type 2 cells; DL1R; DP2; G protein-coupled receptor 44; GPR44; PGD2R; PTGDR2; putative G-protein coupled receptor 44
Related Research Areas

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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A Alexa Fluor® 647-conjugated Isotype Control

Ctrl IC003R
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 10
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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