Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 301109
View all CRTH-2 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human CRTH-2
Met1-Ser395
Accession # Q9Y5Y4

Specificity

Detects human CRTH‑2 in Cell-Based ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human CRTH‑2 APC‑conjugated Antibody

Detection of CRTH-2 antibody in Human Blood Lymphocytes antibody by Flow Cytometry.

Detection of CRTH‑2 in Human Blood Lymphocytes by Flow Cytometry.

Human peripheral blood lymphocytes were stained with Mouse Anti-Human IL-3 Ra/CD123 PE-conjugated Monoclonal Antibody (Catalog # FAB301P) and either (A) Mouse Anti-Human CRTH-2 APC-conjugated Monoclonal Antibody (Catalog # FAB33381A) or (B) Mouse IgG2AAllophycocyanin Isotype Control (Catalog # IC003A). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CRTH‑2 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood lymphocytes

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CRTH-2

CRTH-2, also known as GPR44, is a 7TM G protein-coupled receptor that is expressed by activated Th2 cells and eosinophils. CRTH-2 binds Prostaglandin D2 and induces chemotaxis of these cells in allergic and inflammatory immune responses. Human and mouse CRTH-2 share 78% amino acid sequence identity.

Long Name

Chemoattractant Receptor-homologous Molecule Expressed on Th2 Cells

Alternate Names

CD294, CRTH2, GPR44, PGD2R, PTGDR2

Entrez Gene IDs

11251 (Human); 14764 (Mouse); 309212 (Rat)

Gene Symbol

PTGDR2

UniProt

Q9Y5Y4

Additional CRTH-2 Products

Product Documents for Human CRTH‑2 APC‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CRTH‑2 APC‑conjugated Antibody

For research use only

Customer Reviews for Human CRTH‑2 APC‑conjugated Antibody

There are currently no reviews for this product. Be the first to review Human CRTH‑2 APC‑conjugated Antibody and earn rewards!

Have you used Human CRTH‑2 APC‑conjugated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies