Detection of CRTH‑2 in Human Blood Lymphocytes by Flow Cytometry.
Human peripheral blood lymphocytes were stained with Mouse Anti-Human IL‑3 R alpha /CD123 PE‑conjugated Monoclonal Antibody (Catalog # FAB301P) and either (A) Mouse Anti-Human CRTH‑2 Monoclonal Antibody (Catalog # MAB33381) or (B) Mouse IgG2A Isotype Control (Catalog # MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CRTH-2, also known as GPR44, is a 7TM G protein-coupled receptor that is
expressed by activated Th2 cells and eosinophils. CRTH-2 binds
Prostaglandin D2 and induces chemotaxis of these cells in allergic and
inflammatory immune responses. Human and mouse CRTH-2 share 78% amino
acid sequence identity.
Chemoattractant Receptor-homologous Molecule Expressed on Th2 Cells
Entrez Gene IDs:
11251 (Human); 14764 (Mouse); 309212 (Rat)
CD294 antigen; CD294; Chemoattractant receptor-homologous molecule expressed on Th2 cells; CRTH2; CRTH-2; CRTH2chemoattractant receptor homologous molecule expressed on T helper type 2 cells; DL1R; DP2; G protein-coupled receptor 44; GPR44; PGD2R; PTGDR2; putative G-protein coupled receptor 44
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