Human CTRP9/C1qTNF9 Antibody

Catalog # Availability Size / Price Qty
AF6537-100
AF6537-SP
Detection of Human CTRP9/C1qTNF9 by Western Blot.
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Human CTRP9/C1qTNF9 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CTRP9/C1qTNF9 in direct ELISAs.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CTRP9/C1qTNF9
Asn16-Pro333
Accession # P0C862
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human CTRP9/C1qTNF9 antibody by Western Blot. View Larger

Detection of Human CTRP9/C1qTNF9 by Western Blot. Western blot shows human serum. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human CTRP9/C1qTNF9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6537) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CTRP9/C1qTNF9 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CTRP9/C1qTNF9

C1qTNF9, also known as CTRP9, is one of several Adiponectin/Acrp30 paralogs which comprise the C1q and TNF-related protein family. All family members share a modular organization comprising a short variable region, a collagenous domain, and a C1q-like globular domain. C1qTNF9 is a 40 kDa glycoprotein that contains multiple hydroxylated proline residues in its collagenous region. It circulates as a homotrimer and higher order multimers as well as in heterotrimers with Adiponectin. It is preferentially expressed in adipose tissue and plays a role in glucose homeostasis. Human C1qTNF9 shares 85% amino acid sequence identity with mouse and rat C1qTNF9.

Long Name
C1q and Tumor Necrosis Factor Related Protein 9
Entrez Gene IDs
338872 (Human); 239126 (Mouse)
Alternate Names
AQL1; C1q and tumor necrosis factor related protein 9; C1qTNF9; C1QTNF9AComplement C1q tumor necrosis factor-related protein 9; complement C1q tumor necrosis factor-related protein 9A; CTRP9; MGC48915

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