Detects human CXCL1/2/3 in direct ELISAs and Western blots.
Monoclonal Mouse IgG1 Clone # 31716
Protein A or G purified from ascites
E. coli-derived recombinant human CXCL2 Ala35-Asn107 Accession # P19875
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Recombinant Human CXCL1/GRO alpha /KC/CINC-1 (Catalog # 275-GR) Recombinant Human CXCL2/GRO beta /MIP-2/CINC-3 (Catalog # 276-GB) Recombinant Human CXCL3/GRO gamma /CINC-2/DCIP-1 (Catalog # 277-GG)
Measured by its ability to neutralize CXCL1/2/3/GRO-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.5-2.5 µg/mL, 0.15-0.75µg/mL, and 0.1-0.5 µg/mL for Recombinant Human (rh) CXCL1, rhCXCL2, and rhCXCL3, respectively.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Chemotaxis Induced by CXCL2/GRO beta and Neutralization by Human CXCL1/2/3/GRO Antibody. Recombinant Human CXCL2/GRO beta (Catalog # 176-GB) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL2/GRO beta (6 ng/mL) is neutralized (green line) by increasing concentrations of Human CXCL1/2/3/GRO Monoclonal Antibody (Catalog # MAB276). The ND50 is typically 0.15-0.75 µg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CXCL1, CXCL2 (MIP-2 alpha ), and CXCL3 (MIP-2 beta ) are members of the alpha (C-X-C) subfamily of chemokines and are now designated GRO alpha, GRO beta, and GRO gamma, respectively. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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