Human CXCL1/2/3/GRO Pan Specific Antibody

(6 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human CXCL1/2/3 in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 31716
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    E. coli-derived recombinant human CXCL2
    Ala35-Asn107
    Accession # P19875
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human CXCL1/GRO alpha /KC/CINC-1 (Catalog # 275-GR)
    Recombinant Human CXCL2/GRO beta /MIP-2/CINC-3 (Catalog # 276-GB)
    Recombinant Human CXCL3/GRO gamma /CINC-2/DCIP-1 (Catalog # 277-GG)
  • Neutralization
    Measured by its ability to neutralize CXCL1/2/3/GRO-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.5-2.5 µg/mL, 0.15-0.75µg/mL, and 0.1-0.5 µg/mL for Recombinant Human (rh) CXCL1, rhCXCL2, and rhCXCL3, respectively.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by CXCL2/GRO beta and Neutralization by Human CXCL1/2/3/GRO Antibody. Recombinant Human CXCL2/GRO beta (Catalog # 176-GB) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL2/GRO beta (6 ng/mL) is neutralized (green line) by increasing concentrations of Human CXCL1/2/3/GRO Monoclonal Antibody (Catalog # MAB276). The ND50 is typically 0.15-0.75 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL1/2/3/GRO
CXCL1, CXCL2 (MIP-2 alpha ), and CXCL3 (MIP-2 beta ) are members of the alpha (C-X-C) subfamily of chemokines and are now designated GRO alpha, GRO beta, and GRO gamma, respectively. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators.
  • Alternate Names:
    CXCL1/2/3
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. Podocytes regulate neutrophil recruitment by glomerular endothelial cells via IL-6-mediated crosstalk.
    Authors: Kuravi S, McGettrick H, Satchell S, Saleem M, Harper L, Williams J, Rainger G, Savage C
    J Immunol, 2014;193(1):234-43.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  2. CXCR2-driven ovarian cancer progression involves upregulation of proinflammatory chemokines by potentiating NF-kappaB activation via EGFR-transactivated Akt signaling.
    Authors: Dong Y, Kabir S, Lee E, Son D
    PLoS ONE, 2013;8(12):e83789.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  3. Skin Inflammation Induced by the Synergistic Action of IL-17A, IL-22, Oncostatin M, IL-1alpha, and TNF-alpha Recapitulates Some Features of Psoriasis.
    Authors: Guilloteau K, Paris I, Pedretti N, Boniface K, Juchaux F, Huguier V, Guillet G, Bernard FX, Lecron JC, Morel F
    J. Immunol., 2010;184(0):5263-70.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  4. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
    Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
    Transplantation, 2009;87(12):1814-20.
    Species: Human
    Sample Type: Urine
    Application: Antibody Array Development
  5. L5, the most electronegative subfraction of plasma LDL, induces endothelial vascular cell adhesion molecule 1 and CXC chemokines, which mediate mononuclear leukocyte adhesion.
    Authors: Abe Y, Fornage M, Yang CY, Bui-Thanh NA, Wise V, Chen HH, Rangaraj G, Ballantyne CM
    Atherosclerosis, 2007;192(1):56-66.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  6. Laminar shear stress-induced GRO mRNA and protein expression in endothelial cells.
    Authors: Hagiwara H, Mitsumata M, Yamane T
    Circulation, 1998;98(23):2584-90.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB

FAQs

  1. At what concentration do we use GRO-alpha, GRO-beta, and GRO-gamma for neutralization in bioassay with Catalog # MAB276?

    • Recombinant Human GRO-beta (Catalog # 176-GB) is used at 6 ng/mL, rhGRO-alpha (Catalog # 275-GR) is used at 10 ng/mL, and rhGRO-gamma (Catalog # 277-GG) is used at 20 ng/mL.

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