Human CXCL11/I-TAC Antibody

Catalog #: MAB672
8 Citations Datasheet / COA / SDS

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MAB672-SP
MAB672-100
MAB672-500

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Chemotaxis Induced by CXCL11/I‑TAC and Neutralization by Human CXCL11/I‑TAC Antibody.

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Citations (8)

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Human CXCL11/I-TAC Antibody Summary

Species Reactivity

Human

Specificity

Detects human CXCL11/I-TAC in ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant human (rh) CXCL5, 7, 8, 9, or 10. In Western blots, this antibody shows less than 1% cross-reactivity with rhCXCL1, 2, 3, 5, 6, 7, IL-8/CXCL8, 9, 10, 13, CXCL12/SDF-1 alpha, CXCL12/SDF-1 beta, rmCXCL1, 2, 6, 9, 10, CXCL12/SDF-1 alpha, rmCXCL13, rrCXCL1, CXCL3/CINC-2 alpha, CXCL3/CINC-2 beta, or rpIL-8/CXCL8.

Source

Monoclonal Mouse IgG_2A Clone # 87328

Purification

Protein A or G purified from ascites

Immunogen

E. coli-derived recombinant human CXCL11/I-TAC
Phe22-Phe94
Accession # O14625

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human CXCL11/I-TAC (Catalog # 672-IT)

Human CXCL11/I-TAC Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human CXCL11/I-TAC Biotinylated Antibody (Catalog # BAF672)

Standard: Recombinant Human CXCL11/I-TAC Protein (Catalog # 672-IT)

Neutralization

Measured by its ability to neutralize CXCL11/I‑TAC-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR3. The Neutralization Dose (ND₅₀) is typically 0.2-0.8 µg/mL in the presence of 15 ng/mL Recombinant Human CXCL11/I‑TAC.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Chemotaxis Induced by CXCL11/I‑TAC and Neutralization by Human CXCL11/I‑TAC Antibody. Recombinant Human CXCL11/I‑TAC (Catalog # 672-IT) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL11/I‑TAC (15 ng/mL) is neutralized (green line) by increasing concentrations of Human CXCL11/I-TAC Monoclonal Antibody (Catalog # MAB672). The ND₅₀ is typically 0.2-0.8 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL11/I-TAC

CXCL11, also known as I-TAC, SCYB9B, H174 and beta -R1, is a non-ELR CXC chemokine. CXCL11 cDNA encodes a 94 amino acid (aa) residue precursor protein with a 21 aa residue putative signal sequence, which is cleaved to form the mature 73 aa residue protein. CXCL11 shares 36% and 37% amino acid sequence homology with IP-10 and MIG (two other known human non-ELR CXC chemokines), respectively. CXCL11 is expressed at low levels in normal tissues including thymus, spleen, and pancreas. The expression of CXCL11 mRNA is radically up regulated in IFN-gamma and IL-1 stimulated astrocytes. Moderate increase in expression is also observed in stimulated monocytes. CXCL11 has potent chemoattractant activity for IL-2 activated T cells and transfected cell lines expressing CXCR3 but not freshly isolated T cells, neutrophils, or monocytes. The gene encoding CXCL11 has been mapped to chromosome 4.

References

Cole, K. et al. (1998) J. Exp. Med. 187:2009.
Sandhya Rani, M. et al. (1996) J. Biol. Chem. 271:22878.
Lou, Y. et al. (1998) J. Neurovirol. 4:575.

Entrez Gene IDs

6373 (Human); 56066 (Mouse)

Alternate Names

beta-R1; b-R1; chemokine (C-X-C motif) ligand 11; CXCL11; H174; H174IP9; Interferon gamma-inducible protein 9; Interferon-inducible T-cell alpha chemoattractant; IP-9member 11; ITAC; I-TAC; I-TACMGC102770; SCYB9B; small inducible cytokine subfamily B (Cys-X-Cys), member 9B; Small-inducible cytokine B11

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Citations for Human CXCL11/I-TAC Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Blockade of Immune Checkpoint B7-H4 and Lysine Demethylase 5B in Esophageal Squamous Cell Carcinoma Confers Protective Immunity against P. gingivalis Infection
Authors: X Yuan, Y Liu, G Lee, Z Lan, M Ma, H Lee, J Kong, J Sun, G Hou, X Hou, Y Ma, F Ren, F Zhou, S Gao
Cancer Immunol Res, 2019;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Bioassay
Intracellular coexpression of CXC- and CC- chemokine receptors and their ligands in human melanoma cell lines and dynamic variations after xenotransplantation.
Authors: Pinto S, Martinez-Romero A, O'Connor J, Gil-Benso R, San-Miguel T, Terradez L, Monteagudo C, Callaghan R
BMC Cancer, 2014;14(0):118.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Expression of CXCL9, -10, -11, and CXCR3 in the tear film and ocular surface of patients with dry eye syndrome.
Authors: Yoon KC, Park CS, You IC, Choi HJ, Lee KH, Im SK, Park HY, Pflugfelder SC
Invest. Ophthalmol. Vis. Sci., 2010;51(2):643-50.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver.
Authors: Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH
J. Immunol., 2010;184(6):2886-98.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Down-modulation of CXCR3 surface expression and function in CD8+ T cells from cutaneous T cell lymphoma patients.
Authors: Winter D, Moser J, Kriehuber E, Wiesner C, Knobler R, Trautinger F, Bombosi P, Stingl G, Petzelbauer P, Rot A, Maurer D
J. Immunol., 2007;179(6):4272-82.
Species: Human
Sample Types: Serum
Applications: Immunodepletion
CXCR 3 activation promotes lymphocyte transendothelial migration across human hepatic endothelium under fluid flow.
Authors: Curbishley SM, Eksteen B, Gladue RP, Lalor P, Adams DH
Am. J. Pathol., 2005;167(3):887-99.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot
Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands.
Authors: Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D
J. Immunol., 2004;173(11):6592-602.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
Enhanced generation of helper T type 1 and 2 chemokines in allergen-induced asthma.
Authors: Liu L, Jarjour NN, Busse WW, Kelly EA
Am. J. Respir. Crit. Care Med., 2004;169(10):1118-24.
Species: Human
Sample Types: BALF
Applications: ELISA Development