Human CXCL11/I-TAC Antibody Summary
Accession # O14625
Human CXCL11/I-TAC Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Chemotaxis Induced by CXCL11/I‑TAC and Neutralization by Human CXCL11/I‑TAC Antibody. Recombinant Human CXCL11/I‑TAC (Catalog # 672-IT) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL11/I‑TAC (15 ng/mL) is neutralized (green line) by increasing concentrations of Human CXCL11/I-TAC Monoclonal Antibody (Catalog # MAB672). The ND50 is typically 0.2-0.8 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
CXCL11, also known as I-TAC, SCYB9B, H174 and beta -R1, is a non-ELR CXC chemokine. CXCL11 cDNA encodes a 94 amino acid (aa) residue precursor protein with a 21 aa residue putative signal sequence, which is cleaved to form the mature 73 aa residue protein. CXCL11 shares 36% and 37% amino acid sequence homology with IP-10 and MIG (two other known human non-ELR CXC chemokines), respectively. CXCL11 is expressed at low levels in normal tissues including thymus, spleen, and pancreas. The expression of CXCL11 mRNA is radically up regulated in IFN-gamma and IL-1 stimulated astrocytes. Moderate increase in expression is also observed in stimulated monocytes. CXCL11 has potent chemoattractant activity for IL-2 activated T cells and transfected cell lines expressing CXCR3 but not freshly isolated T cells, neutrophils, or monocytes. The gene encoding CXCL11 has been mapped to chromosome 4.
- Cole, K. et al. (1998) J. Exp. Med. 187:2009.
- Sandhya Rani, M. et al. (1996) J. Biol. Chem. 271:22878.
- Lou, Y. et al. (1998) J. Neurovirol. 4:575.
Citations for Human CXCL11/I-TAC Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 8
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Blockade of Immune Checkpoint B7-H4 and Lysine Demethylase 5B in Esophageal Squamous Cell Carcinoma Confers Protective Immunity against P. gingivalis Infection
Authors: X Yuan, Y Liu, G Lee, Z Lan, M Ma, H Lee, J Kong, J Sun, G Hou, X Hou, Y Ma, F Ren, F Zhou, S Gao
Cancer Immunol Res, 2019;0(0):.
Sample Types: Cell Culture Supernates
Intracellular coexpression of CXC- and CC- chemokine receptors and their ligands in human melanoma cell lines and dynamic variations after xenotransplantation.
Authors: Pinto S, Martinez-Romero A, O'Connor J, Gil-Benso R, San-Miguel T, Terradez L, Monteagudo C, Callaghan R
BMC Cancer, 2014;14(0):118.
Sample Types: Whole Cells
Applications: Flow Cytometry
Expression of CXCL9, -10, -11, and CXCR3 in the tear film and ocular surface of patients with dry eye syndrome.
Authors: Yoon KC, Park CS, You IC, Choi HJ, Lee KH, Im SK, Park HY, Pflugfelder SC
Invest. Ophthalmol. Vis. Sci., 2010;51(2):643-50.
Sample Types: Whole Tissue
Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver.
Authors: Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH
J. Immunol., 2010;184(6):2886-98.
Sample Types: Whole Cells
Down-modulation of CXCR3 surface expression and function in CD8+ T cells from cutaneous T cell lymphoma patients.
Authors: Winter D, Moser J, Kriehuber E, Wiesner C, Knobler R, Trautinger F, Bombosi P, Stingl G, Petzelbauer P, Rot A, Maurer D
J. Immunol., 2007;179(6):4272-82.
Sample Types: Serum
CXCR 3 activation promotes lymphocyte transendothelial migration across human hepatic endothelium under fluid flow.
Authors: Curbishley SM, Eksteen B, Gladue RP, Lalor P, Adams DH
Am. J. Pathol., 2005;167(3):887-99.
Sample Types: Tissue Homogenates
Applications: Western Blot
Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands.
Authors: Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D
J. Immunol., 2004;173(11):6592-602.
Sample Types: Whole Tissue
Enhanced generation of helper T type 1 and 2 chemokines in allergen-induced asthma.
Authors: Liu L, Jarjour NN, Busse WW, Kelly EA
Am. J. Respir. Crit. Care Med., 2004;169(10):1118-24.
Sample Types: BALF
Applications: ELISA Development
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