Human CXCL11/I-TAC Quantikine ELISA Kit

Catalog # Availability Size / Price Qty
DCX110
Control Products Available
Human CXCL11/I-TAC ELISA Cell Culture Supernate Standard Curve
3 Images
Product Details
Procedure
Citations (24)
FAQs
Supplemental Products
Reviews (1)

Human CXCL11/I-TAC Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
Sensitivity
39.7 pg/mL
Assay Range
62.5 - 4,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human I-TAC
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human I-TAC Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human I-TAC in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human I-TAC and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human I-TAC showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human I-TAC.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 353 1179 2191 409 1290 2401
Standard Deviation 18 40.6 102.9 35.5 93 179
CV% 5.1 3.4 4.7 8.7 7.2 7.5

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 403 1321 2481 437 1403 2644
Standard Deviation 19 62.5 147.3 35.1 107 190
CV% 4.7 4.7 5.9 8 7.6 7.2

Recovery

The recovery of I-TAC spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 100 91-111
EDTA Plasma (n=5) 98 85-112
Heparin Plasma (n=5) 98 90-106
Serum (n=5) 106 93-117

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of I-TAC were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human CXCL11/I-TAC ELISA Linearity

Data Examples

Human CXCL11/I-TAC ELISA Cell Culture Supernate Standard Curve

Human CXCL11/I-TAC ELISA Serum/Plasma Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL11/I-TAC

Interferon-inducible T cell a chemoattractant (I-TAC), also known as SCYB9B, H174 and beta-R1, is a non-ELR motif-containing CXC chemokine. I-TAC shares 36% and 37% amino acid sequence homology with IP-10 and MIG, respectively. I-TAC is expressed at low levels in normal tissues, including thymus, spleen and pancreas.

Entrez Gene IDs:
6373 (Human); 56066 (Mouse)
Alternate Names:
beta-R1; b-R1; chemokine (C-X-C motif) ligand 11; CXCL11; H174; H174IP9; Interferon gamma-inducible protein 9; Interferon-inducible T-cell alpha chemoattractant; IP-9member 11; ITAC; I-TAC; I-TACMGC102770; SCYB9B; small inducible cytokine subfamily B (Cys-X-Cys), member 9B; Small-inducible cytokine B11
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 100 µL Standard, Control, or Sample
  6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human CXCL11/I-TAC Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

24 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Obesity in acute alcoholic hepatitis increases morbidity and mortality
    Authors: R Parker, SJ Kim, GY Im, J Nahas, B Dhesi, N Vergis, A Sinha, A Ghezzi, MR Rink, A McCune, GP Aithal, PN Newsome, CJ Weston, A Holt, B Gao
    EBioMedicine, 2019;45(0):511-518.
    Species: Human
    Sample Types: Serum
  2. Salt suppresses IFN? inducible chemokines through the IFN?-JAK1-STAT1 signaling pathway in proximal tubular cells
    Authors: Y Arai, D Takahashi, K Asano, M Tanaka, M Oda, SBH Ko, MSH Ko, S Mandai, N Nomura, T Rai, S Uchida, E Sohara
    Sci Rep, 2017;7(0):46580.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Inflammatory Differences in Plaque Erosion and Rupture in Patients With ST-Segment Elevation Myocardial Infarction
    Authors: S Chandran, J Watkins, A Abdul-Aziz, M Shafat, PA Calvert, KM Bowles, MD Flather, SA Rushworth, AD Ryding
    J Am Heart Assoc, 2017;6(5):.
    Species: Human
    Sample Types: Plasma
  4. Effective Apical Infection of Differentiated Human Bronchial Epithelial Cells and Induction of Proinflammatory Chemokines by the Highly Pneumotropic Human Adenovirus Type 14p1.
    Authors: Lam E, Ramke M, Warnecke G, Schrepfer S, Kopfnagel V, Dobner T, Heim A
    PLoS ONE, 2015;10(7):e0131201.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Left Ventricular Dysfunction and CXCR3 Ligands in Hypertension: From Animal Experiments to a Population-Based Pilot Study.
    Authors: Altara R, Gu Y, Struijker-Boudier H, Thijs L, Staessen J, Blankesteijn W
    PLoS ONE, 2015;10(10):e0141394.
    Species: Human
    Sample Types: Plasma
  6. Expression of CXCR3 and its ligands CXCL9, -10 and -11 in paediatric opsoclonus-myoclonus syndrome.
    Authors: Pranzatelli M, Tate E, McGee N, Travelstead A, Verhulst S, Ransohoff R
    Clin Exp Immunol, 2013;172(3):427-36.
    Species: Human
    Sample Types: Serum
  7. Human cytomegalovirus IE1 protein elicits a type II interferon-like host cell response that depends on activated STAT1 but not interferon-gamma.
    Authors: Knoblach T, Grandel B, Seiler J, Nevels M, Paulus C
    PLoS Pathog., 2011;7(4):e1002016.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. NOD1 contributes to mouse host defense against Helicobacter pylori via induction of type I IFN and activation of the ISGF3 signaling pathway.
    Authors: Watanabe T, Asano N, Fichtner-Feigl S, Gorelick PL, Tsuji Y, Matsumoto Y, Chiba T, Fuss IJ, Kitani A, Strober W
    J. Clin. Invest., 2010;120(5):1645-62.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. Expression of CXCL9, -10, -11, and CXCR3 in the tear film and ocular surface of patients with dry eye syndrome.
    Authors: Yoon KC, Park CS, You IC, Choi HJ, Lee KH, Im SK, Park HY, Pflugfelder SC
    Invest. Ophthalmol. Vis. Sci., 2010;51(2):643-50.
    Species: Human
    Sample Types: Tears
  10. The imbalance in serum concentration of Th-1- and Th-2-derived chemokines as one of the factors involved in pathogenesis of atopic dermatitis.
    Authors: Narbutt J, Lesiak A, Sysa-Jedrzeiowska A, Zakrzewski M, Bogaczewicz J, Stelmach I, Kuna P
    Mediators Inflamm., 2009;2009(0):269541.
    Species: Human
    Sample Types: Serum
  11. CXCR3 ligands are augmented during the pathogenesis of pulmonary sarcoidosis.
    Authors: Busuttil A, Weigt SS, Keane MP, Xue YY, Palchevskiy V, Burdick MD, Huang C, Zisman DA, Fishbein M, Lynch JP, Strieter RM, Elashoff RM, Belperio JA
    Eur. Respir. J., 2009;34(3):676-86.
    Species: Human
    Sample Types: BALF
  12. Villitis of unknown etiology is associated with a distinct pattern of chemokine up-regulation in the feto-maternal and placental compartments: implications for conjoint maternal allograft rejection and maternal anti-fetal graft-versus-host disease.
    Authors: Kim MJ, Romero R, Kim CJ, Tarca AL, Chhauy S, LaJeunesse C, Lee DC, Draghici S, Gotsch F, Kusanovic JP, Hassan SS, Kim JS
    J. Immunol., 2009;182(6):3919-27.
    Species: Human
    Sample Types: Plasma
  13. Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases.
    Authors: Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D
    J. Immunol., 2008;181(11):8053-67.
    Species: Human
    Sample Types: BALF
  14. The antibacterial chemokine MIG/CXCL9 is constitutively expressed in epithelial cells of the male urogenital tract and is present in seminal plasma.
    Authors: Linge HM, Collin M, Giwercman A, Malm J, Bjartell A, Egesten A
    J. Interferon Cytokine Res., 2008;28(3):191-6.
    Species: Human
    Sample Types: Seminal Plasma
  15. Prolactin enhances interferon-gamma-induced production of CXC ligand 9 (CXCL9), CXCL10, and CXCL11 in human keratinocytes.
    Authors: Kanda N, Watanabe S
    Endocrinology, 2007;148(5):2317-25.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. Th1/Th2 cytokines reciprocally regulate in vitro pulmonary angiogenesis via CXC chemokine synthesis.
    Authors: Matsuda A, Fukuda S, Matsumoto K, Saito H
    Am. J. Respir. Cell Mol. Biol., 2007;38(2):168-75.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. Chemokines CXCL10 and CXCL11 in the cerebrospinal fluid of patients with tick-borne encephalitis.
    Authors: Lepej SZ, Misic-Majerus L, Jeren T, Rode OD, Remenar A, Sporec V, Vince A
    Acta Neurol. Scand., 2007;115(2):109-14.
    Species: Human
    Sample Types: Serum
  18. CXCR3 and CCR5 chemokines in induced sputum from patients with COPD.
    Authors: Costa C, Rufino R, Traves SL, Lapa E Silva JR, Barnes PJ, Donnelly LE
    Chest, 2007;133(1):26-33.
    Species: Human
    Sample Types: Saliva
  19. IFN-gamma alters the response of Borrelia burgdorferi-activated endothelium to favor chronic inflammation.
    Authors: Dame TM, Orenzoff BL, Palmer LE, Furie MB
    J. Immunol., 2007;178(2):1172-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  20. Different angiogenic activity in pulmonary sarcoidosis and idiopathic pulmonary fibrosis.
    Authors: Antoniou KM, Tzouvelekis A, Alexandrakis MG, Sfiridaki K, Tsiligianni I, Rachiotis G, Tzanakis N, Bouros D, Milic-Emili J, Siafakas NM
    Chest, 2006;130(4):982-8.
    Species: Human
    Sample Types: BALF
  21. Increased expression of CXCR3 and CCR5 on memory CD4+ T-cells migrating into the cerebrospinal fluid of patients with neuroborreliosis: the role of CXCL10 and CXCL11.
    Authors: Lepej SZ, Rode OD, Jeren T, Vince A, Remenar A, Barsic B
    J. Neuroimmunol., 2005;163(1):128-34.
    Species: Human
    Sample Types: Serum
  22. IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
    Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
    J. Immunol., 2005;175(9):6177-89.
    Species: Human
    Sample Types: Cell Culture Supernates
  23. CXCR3 and CCR5 positive T-cell recruitment in acute human renal allograft rejection.
    Authors: Panzer U, Reinking RR, Steinmetz OM, Zahner G, Sudbeck U, Fehr S, Pfalzer B, Schneider A, Thaiss F, Mack M, Conrad S, Huland H, Helmchen U, Stahl RA
    Transplantation, 2004;78(9):1341-50.
    Species: Human
    Sample Types: Urine
  24. VSIG-3 as a ligand of VISTA inhibits human T-cell function.
    Authors: Wang J, Wu G, Manick B, Hernandez V, Renelt M, Erickson C, Guan J, Singh R, Rollins S, Solorz A, Bi M, Li J, Grabowski D, Dirkx J, Tracy C, Stuart T, Ellinghuysen C, Desmond D, Foster C, Kalabokis V
    Immunology, 0;156(1):74-85.
    Species: Human
    Sample Types: Cell Culture Supernates

FAQs

No product specific FAQs exist for this product, however you may

View all ELISA FAQs

Reviews for Human CXCL11/I-TAC Quantikine ELISA Kit

Average Rating: 5 (Based on 1 Review)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human CXCL11/I-TAC Quantikine ELISA Kit?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human CXCL11/I-TAC Quantikine ELISA Kit
By Anonymous on 07/11/2019
Sample Tested: BMEC