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Human CXCL11/I-TAC Quantikine ELISA Kit

R&D Systems | Catalog # DCX110

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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL)

Sensitivity

39.7 pg/mL

Assay Range

62.5-4000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
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Product Summary for Human CXCL11/I-TAC Quantikine ELISA Kit

The Quantikine Human I-TAC Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human I-TAC in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human I-TAC and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human I-TAC showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human I-TAC.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human I-TAC

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 353 1179 2191 409 1290 2401
Standard Deviation 18 40.6 102.9 35.5 93 179
CV% 5.1 3.4 4.7 8.7 7.2 7.5

EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 403 1321 2481 437 1403 2644
Standard Deviation 19 62.5 147.3 35.1 107 190
CV% 4.7 4.7 5.9 8.0 7.6 7.2

Recovery for Human CXCL11/I-TAC Quantikine ELISA Kit

The recovery of I-TAC spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 100 91-111
EDTA Plasma (n=5) 98 85-112
Heparin Plasma (n=5) 98 90-106
Serum (n=5) 106 93-117

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of I-TAC were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human CXCL11/I-TAC ELISA Linearity

Scientific Data Images for Human CXCL11/I-TAC Quantikine ELISA Kit

Human CXCL11/I-TAC ELISA Cell Culture Supernate Standard Curve

Human CXCL11/I-TAC ELISA Cell Culture Supernate Standard Curve

Human CXCL11/I-TAC ELISA Serum/Plasma Standard Curve

Human CXCL11/I-TAC ELISA Serum/Plasma Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL11/I-TAC

Interferon-inducible T cell a chemoattractant (I-TAC), also known as SCYB9B, H174 and beta-R1, is a non-ELR motif-containing CXC chemokine. I-TAC shares 36% and 37% amino acid sequence homology with IP-10 and MIG, respectively. I-TAC is expressed at low levels in normal tissues, including thymus, spleen and pancreas.

Alternate Names

beta-R1, H174, I-TAC, ITAC, SCYB9B

Entrez Gene IDs

6373 (Human); 56066 (Mouse)

Gene Symbol

CXCL11

Additional CXCL11/I-TAC Products

Product Documents for Human CXCL11/I-TAC Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CXCL11/I-TAC Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human CXCL11/I-TAC Quantikine ELISA Kit

Customer Reviews for Human CXCL11/I-TAC Quantikine ELISA Kit (2)

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  • Human CXCL11/I-TAC Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Plasma and Urine
    Verified Customer | Posted 11/07/2022
    Not detected in urine at 2-fold. Detected in low concentration in plasma at 10-fold. No matrix effects detected.
    Human CXCL11/I-TAC Quantikine ELISA Kit DCX110
  • Human CXCL11/I-TAC Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: BMEC
    Verified Customer | Posted 07/11/2019

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Protocols

View specific protocols for Human CXCL11/I-TAC Quantikine ELISA Kit (DCX110):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 100 µL Standard, Control, or Sample
  4.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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FAQs

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