Human CXCL11/I-TAC Quantikine ELISA Kit

(19 citations)   
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
  • Sensitivity
    39.7 pg/mL
  • Assay Range
    62.5 - 4,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant human I-TAC
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
Product Summary
The Quantikine Human I-TAC Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human I-TAC in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human I-TAC and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human I-TAC showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human I-TAC.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Standard Deviation1840.6102.935.593179

Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Standard Deviation1962.5147.335.1107190


The recovery of I-TAC spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 100 91-111
EDTA Plasma (n=5) 98 85-112
Heparin Plasma (n=5) 98 90-106
Serum (n=5) 106 93-117
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of I-TAC were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human CXCL11/I-TAC Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CXCL11/I-TAC
Interferon-inducible T cell a chemoattractant (I-TAC), also known as SCYB9B, H174 and beta-R1, is a non-ELR motif-containing CXC chemokine. I-TAC shares 36% and 37% amino acid sequence homology with IP-10 and MIG, respectively. I-TAC is expressed at low levels in normal tissues, including thymus, spleen and pancreas.
    • Entrez Gene IDs
      6373 (Human); 56066 (Mouse);
    • Alternate Names
      beta-R1; b-R1; chemokine (C-X-C motif) ligand 11; H174; H174IP9; Interferon gamma-inducible protein 9; Interferon-inducible T-cell alpha chemoattractant; IP-9member 11; ITAC; I-TAC; I-TACMGC102770; SCYB9B; small inducible cytokine subfamily B (Cys-X-Cys), member 9B; Small-inducible cytokine B11;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 100 µL Standard, Control, or Sample
    6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 10 of 19
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    Sample Type
    1. Left Ventricular Dysfunction and CXCR3 Ligands in Hypertension: From Animal Experiments to a Population-Based Pilot Study.
      Authors: Altara R, Gu Y, Struijker-Boudier H, Thijs L, Staessen J, Blankesteijn W
      PLoS ONE, 2015;10(10):e0141394.
      Species: Human
      Sample Type: Plasma
    2. Expression of CXCR3 and its ligands CXCL9, -10 and -11 in paediatric opsoclonus-myoclonus syndrome.
      Authors: Pranzatelli M, Tate E, McGee N, Travelstead A, Verhulst S, Ransohoff R
      Clin Exp Immunol, 2013;172(3):427-36.
      Species: Human
      Sample Type: Serum
    3. Human cytomegalovirus IE1 protein elicits a type II interferon-like host cell response that depends on activated STAT1 but not interferon-gamma.
      Authors: Knoblach T, Grandel B, Seiler J, Nevels M, Paulus C
      PLoS Pathog., 2011;7(4):e1002016.
      Species: Human
      Sample Type: Cell Culture Supernates
    4. Expression of CXCL9, -10, -11, and CXCR3 in the tear film and ocular surface of patients with dry eye syndrome.
      Authors: Yoon KC, Park CS, You IC, Choi HJ, Lee KH, Im SK, Park HY, Pflugfelder SC
      Invest. Ophthalmol. Vis. Sci., 2010;51(2):643-50.
      Species: Human
      Sample Type: Tears
    5. NOD1 contributes to mouse host defense against Helicobacter pylori via induction of type I IFN and activation of the ISGF3 signaling pathway.
      Authors: Watanabe T, Asano N, Fichtner-Feigl S, Gorelick PL, Tsuji Y, Matsumoto Y, Chiba T, Fuss IJ, Kitani A, Strober W
      J. Clin. Invest., 2010;120(5):1645-62.
      Species: Human
      Sample Type: Cell Culture Supernates
    6. CXCR3 ligands are augmented during the pathogenesis of pulmonary sarcoidosis.
      Authors: Busuttil A, Weigt SS, Keane MP, Xue YY, Palchevskiy V, Burdick MD, Huang C, Zisman DA, Fishbein M, Lynch JP, Strieter RM, Elashoff RM, Belperio JA
      Eur. Respir. J., 2009;34(3):676-86.
      Species: Human
      Sample Type: BALF
    7. Villitis of unknown etiology is associated with a distinct pattern of chemokine up-regulation in the feto-maternal and placental compartments: implications for conjoint maternal allograft rejection and maternal anti-fetal graft-versus-host disease.
      Authors: Kim MJ, Romero R, Kim CJ, Tarca AL, Chhauy S, LaJeunesse C, Lee DC, Draghici S, Gotsch F, Kusanovic JP, Hassan SS, Kim JS
      J. Immunol., 2009;182(6):3919-27.
      Species: Human
      Sample Type: Plasma
    8. The imbalance in serum concentration of Th-1- and Th-2-derived chemokines as one of the factors involved in pathogenesis of atopic dermatitis.
      Authors: Narbutt J, Lesiak A, Sysa-Jedrzeiowska A, Zakrzewski M, Bogaczewicz J, Stelmach I, Kuna P
      Mediators Inflamm., 2009;2009(0):269541.
      Species: Human
      Sample Type: Serum
    9. The antibacterial chemokine MIG/CXCL9 is constitutively expressed in epithelial cells of the male urogenital tract and is present in seminal plasma.
      Authors: Linge HM, Collin M, Giwercman A, Malm J, Bjartell A, Egesten A
      J. Interferon Cytokine Res., 2008;28(3):191-6.
      Species: Human
      Sample Type: seminal plasma
    10. Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases.
      Authors: Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D
      J. Immunol., 2008;181(11):8053-67.
      Species: Human
      Sample Type: BALF
    11. CXCR3 and CCR5 chemokines in induced sputum from patients with COPD.
      Authors: Costa C, Rufino R, Traves SL, Lapa E Silva JR, Barnes PJ, Donnelly LE
      Chest, 2007;133(1):26-33.
      Species: Human
      Sample Type: Saliva
    12. IFN-gamma alters the response of Borrelia burgdorferi-activated endothelium to favor chronic inflammation.
      Authors: Dame TM, Orenzoff BL, Palmer LE, Furie MB
      J. Immunol., 2007;178(2):1172-9.
      Species: Human
      Sample Type: Cell Culture Supernates
    13. Prolactin enhances interferon-gamma-induced production of CXC ligand 9 (CXCL9), CXCL10, and CXCL11 in human keratinocytes.
      Authors: Kanda N, Watanabe S
      Endocrinology, 2007;148(5):2317-25.
      Species: Human
      Sample Type: Cell Culture Supernates
    14. Th1/Th2 cytokines reciprocally regulate in vitro pulmonary angiogenesis via CXC chemokine synthesis.
      Authors: Matsuda A, Fukuda S, Matsumoto K, Saito H
      Am. J. Respir. Cell Mol. Biol., 2007;38(2):168-75.
      Species: Human
      Sample Type: Cell Culture Supernates
    15. Chemokines CXCL10 and CXCL11 in the cerebrospinal fluid of patients with tick-borne encephalitis.
      Authors: Lepej SZ, Misic-Majerus L, Jeren T, Rode OD, Remenar A, Sporec V, Vince A
      Acta Neurol. Scand., 2007;115(2):109-14.
      Species: Human
      Sample Type: Serum
    16. Different angiogenic activity in pulmonary sarcoidosis and idiopathic pulmonary fibrosis.
      Authors: Antoniou KM, Tzouvelekis A, Alexandrakis MG, Sfiridaki K, Tsiligianni I, Rachiotis G, Tzanakis N, Bouros D, Milic-Emili J, Siafakas NM
      Chest, 2006;130(4):982-8.
      Species: Human
      Sample Type: BALF
    17. IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
      Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
      J. Immunol., 2005;175(9):6177-89.
      Species: Human
      Sample Type: Cell Culture Supernates
    18. Increased expression of CXCR3 and CCR5 on memory CD4+ T-cells migrating into the cerebrospinal fluid of patients with neuroborreliosis: the role of CXCL10 and CXCL11.
      Authors: Lepej SZ, Rode OD, Jeren T, Vince A, Remenar A, Barsic B
      J. Neuroimmunol., 2005;163(1):128-34.
      Species: Human
      Sample Type: Serum
    19. CXCR3 and CCR5 positive T-cell recruitment in acute human renal allograft rejection.
      Authors: Panzer U, Reinking RR, Steinmetz OM, Zahner G, Sudbeck U, Fehr S, Pfalzer B, Schneider A, Thaiss F, Mack M, Conrad S, Huland H, Helmchen U, Stahl RA
      Transplantation, 2004;78(9):1341-50.
      Species: Human
      Sample Type: Urine
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