Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Direct ELISA, Flow Cytometry, Immunocytochemistry, ELISA Development, Immuno-PCR

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 53610
View all CXCL13/BLC/BCA-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CXCL13/BLC/BCA-1
Val23-Arg94
Accession # O43927

Specificity

Detects human CXCL13/BLC/BCA-1 in ELISAs and Western blots. In ELISAs, no cross-reactivity with recombinant human (rh) CXCL1, rhCXCL2, rhCXCL3, rhCXCL8/IL‑8, rhCXCL10, rhCXCL9, rhCXCL12/SDF‑1 alpha, or rhCXCL12/SDF‑1 beta is observed. In Western blots, no cross‑reactivity with recombinant mouse CXCL13/BLC/BCA-1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human CXCL13/BLC/BCA‑1 Antibody

Detection of Recombinant Human CXCL13/BLC/BCA-1 antibody by Western Blot.

Detection of Recombinant Human CXCL13/BLC/BCA‑1 by Western Blot.

Western blot shows 100 ng of Recombinant Human CXCL13/BLC/BCA-1 (Catalog # 801-CX) and Recombinant Mouse CXCL13/BLC/BCA-1 (Catalog # 470-BC). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human CXCL13/BLC/BCA-1 Monoclonal Antibody (Catalog # MAB801) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CXCL13/BLC/BCA-1 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Chemotaxis Induced by CXCL13/BLC/BCA‑1 and Neutralization by Human CXCL13/BLC/BCA‑1 Antibody.

Chemotaxis Induced by CXCL13/BLC/BCA‑1 and Neutralization by Human CXCL13/BLC/BCA‑1 Antibody.

Recombinant Human CXCL13/BLC/BCA-1 (Catalog # 801-CX) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse Anti-Human CXCL13/BLC/BCA-1 (0.05 µg/mL) is neutralized (green line) by increasing concentrations of Human CXCL13/BLC/BCA-1 Monoclonal Antibody (Catalog # MAB801). The ND50 is typically 0.3-0.9 µg/mL.
Detection of Human CXCL13/BLC/BCA-1 by Flow Cytometry

Detection of Human CXCL13/BLC/BCA-1 by Flow Cytometry

Relationship of different CD8+ TRM subset with B cells and TLS in CRC tumor tissues. (A) Volcano plot showing differentially expressed genes between DP CD8+ TRM subsets and SP CD8+ TRM subsets of the GSE108989 dataset. (B) The FFPE CRC tumor tissue slides from patients with CRC were stained with DAPI (blue), CD20 (green), CD8 (yellow), CD103 (orange), and CD69 (red). (C) Relationship between the proportion of different CD8+ TRM cells and the proportion of B cells in CRC tumor tissues detected by flow cytometry (n = 40) and relationship between the proportion of different CD8+ TRM cells and the count of B cells and the count and area of TLS in CRC tumor tissues detected by mIHC (n = 64). Statistical analysis was performed by Pearson correlation analysis. (D) Schematic overview of the transwell migration assay design. (E) B cell migration rate when cultured either alone or with different CD8+ TRM subsets (n = 10) and B cell migration rate when cultured with DP CD8+ TRM cells with or without anti-CXCL13 neutralizing antibody (n = 7). Statistical analysis was performed by non-parametric Wilcoxon’s matched-pairs tests and Friedman test with Dunn’s multiple comparisons test. (*p < 0.05; **p < 0.01) Image collected and cropped by CiteAb from the following open publication (https://biosignaling.biomedcentral.com/articles/10.1186/s12964-024-0199…), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human CXCL13/BLC/BCA‑1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human CXCL13/BLC/BCA‑1 (Catalog # 801-CX)

Neutralization

Measured by its ability to neutralize CXCL13/BLC/BCA‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR5. The Neutralization Dose (ND50) is typically 0.3-0.9 µg/mL in the presence of 0.05 µg/mL Recombinant Human CXCL13/BLC/BCA‑1.

Human CXCL13/BLC/BCA-1 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human CXCL13/BLC/BCA‑1 Biotinylated Antibody (Catalog # BAF801)
  • Standard: Recombinant Human CXCL13/BLC/BCA-1 Protein (Catalog # 801-CX)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 4 reviews rated 4.5 using MAB801 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXCL13/BLC/BCA-1

CXCL13, also known as B-lymphocyte chemoattractant (BLC), is a CXC chemokine that is constitutively expressed in secondary lymphoid organs. BCA-1 cDNA encodes a protein of 109 amino acid residues with a leader sequence of 22 residues. Mature human BCA-1 shares 64% amino acid sequence similarity with the mouse protein and 23-34% amino acid sequence identity with other known CXC chemokines. Recombinant or chemically synthesized BCA-1 is a potent chemoattractant for B lymphocytes but not T lymphocytes, monocytes or neutrophils. BLR1, a G protein-coupled receptor originally isolated from Burkitt’s lymphoma cells, has now been shown to be the specific receptor for BCA-1. Among cells of the hematopoietic lineages, the expression of BLR1, now designated CXCR5, is restricted to B lymphocytes and a subpopulation of T helper memory cells. Mice lacking BLR1 have been shown to lack inguinal lymph nodes. These mice were also found to have impaired development of Peyer’s patches and defective formation of primary follicles and germinal centers in the spleen as a result of the inability of B lymphocytes to migrate into B cell areas.

References

  1. Gunn, M.D. et al. (1998) Nature, 391:799.
  2. Legler, D.F. et al. (1998) J. Exp. Med. 187:655.
  3. Forster, R. et al. (1996) Cell 87:1037.

Alternate Names

ANGIE2, BCA-1, BCA1, BLC, BLR1L, SCYB13

Entrez Gene IDs

10563 (Human); 55985 (Mouse)

Gene Symbol

CXCL13

UniProt

O43927

Additional CXCL13/BLC/BCA-1 Products

Product Documents for Human CXCL13/BLC/BCA‑1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CXCL13/BLC/BCA‑1 Antibody

For research use only

Citations for Human CXCL13/BLC/BCA‑1 Antibody

Customer Reviews for Human CXCL13/BLC/BCA‑1 Antibody (4)

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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • Human CXCL13/BLC/BCA-1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Adult brain
    Species: Primate
    Verified Customer | Posted 04/09/2025
    Human CXCL13/BLC/BCA‑1 Antibody MAB801
    Bio-Techne Response
    This review reflects a new species or application tested on a primary antibody.
  • Human CXCL13/BLC/BCA-1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Tonsil tissue
    Species: Human
    Verified Customer | Posted 02/07/2020
    IHC-FFPE section pretreatment: heating in acidic buffer (BOND ER1, Leica) HIER in basic buffer is not suitable primary Ab: 10ug/mL (x50) diluted in Renoir Red Diluent (Biocare), incubation for 30min Detection:Leica Bond Refine polymer
    Human CXCL13/BLC/BCA‑1 Antibody MAB801
  • Human CXCL13/BLC/BCA-1 Antibody
    Name: Anonymous
    Application: Cell depletion
    Sample Tested: Peripheral blood mononuclear cells (PBMCs)
    Species: Human
    Verified Customer | Posted 04/23/2018
  • Human CXCL13/BLC/BCA-1 Antibody
    Name: Anonymous
    Application: Cell depletion
    Sample Tested: Cell Culture Supernates
    Species: Human
    Verified Customer | Posted 06/28/2016

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FAQs for Human CXCL13/BLC/BCA‑1 Antibody

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  • Q: What is the light chain of Human CXCL13/BLC/BCA-1 Antibody, Catalog # MAB801, Clone # 53610?

    A: Catalog # MAB801 has a kappa light chain.

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