Human CXCL5/ENA-78 Quantikine ELISA Kit

(20 citations)   
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (50 uL), Platelet-poor Heparin Plasma (50 uL), Citrate Plasma (50 uL)
  • Sensitivity
    15 pg/mL
  • Assay Range
    31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Platelet-poor Heparin Plasma, Citrate Plasma)
  • Specificity
    Natural and recombinant human ENA-78
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
Product Summary
The Quantikine Human ENA-78 Immunoassay is a 4.5 hour solid phase ELISA designed to measure human ENA-78 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human ENA-78 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant human ENA-78 accurately. Results obtained using natural human ENA-78 showed dose curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human ENA-78.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Platelet-poor Heparin Plasma, Citrate Plasma
Intra-Assay Precision Inter-Assay Precision
Standard Deviation9.412.937.810.117.865.8

Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Standard Deviation614.644.912.626.896.5


The recovery of ENA-78 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 101 95-107
Citrate Plasma (n=5) 100 94-106
EDTA Plasma (n=5) 98 92-104
Heparin Plasma (n=5) 98 93-103
Serum (n=5) 101 93-109
To assess the linearity of the assay, five samples containing or spiked with high concentrations of ENA-78 in various matrices were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human CXCL5/ENA-78 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CXCL5/ENA-78
CXCL5/Epithelial Cell-derived Neutrophil-activating Peptide (ENA-78), is a member of the CXC subfamily of chemokines. Full-length CXCL5/ENA-78 is 114 amino acids (aa) in length with a predicted molecular weight of 12 kDa. Following the removal of the signal peptide, bioactive CXCL5/ENA-78 is 78 aa in length. CXCL5/ENA-78 can be N-terminally cleaved by Cathepsin G and Chymotrypsin to CXCL5/ENA-74 (74 aa) and CXCL5/ENA-70 (70 aa), which show increased potency relative to CXCL5/ENA-78. While murine LIX was thought to be an ortholog to human CXCL5/ENA-78, genome-wide analysis and a consensus in the field suggests that human CXCL5/ENA-78 does not have a true murine ortholog. CXCL5/ENA-78 is upregulated at sites of inflammation and is expressed by multiple hematopoietic cell types, fibroblasts, endothelial cells, vascular smooth muscle cells, and adipocytes. It acts as a chemoattractant for neutrophils, has angiogenic properties, and contributes to cancer progression.
    • Entrez Gene IDs
      6374 (Human);
    • Alternate Names
      CXCL5; ENA78; ENA-78;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 200 µL Assay Diluent
    4.   Add 200 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process twice for a total of 3 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 3 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 10 of 20
    Filter your results:

    Sample Type
    1. Caspase 3 involves in neuroplasticity, microglial activation and neurogenesis in the mice hippocampus after intracerebral injection of kainic acid.
      Authors: Tzeng, Tsai-Ten, Tsay, Huey-Jen, Chang, Luping, Hsu, Chia-Lin, Lai, Tzu-Hsua, Huang, Fong-Lee, Shiao, Young-Ji
      J Biomed Sci, 2013;20(0):90.
      Species: Human
      Sample Type: Cell Culture Supernates
    2. Decreased plasma cytokines are associated with low platelet counts in aplastic anemia and immune thrombocytopenic purpura.
      J. Thromb. Haemost., 2012;10(8):1616-23.
      Species: Human
      Sample Type: Serum
    3. Inhibition of IL-1beta-mediated inflammatory responses by the IkappaB alpha super-repressor in human fibroblast-like synoviocytes.
      Authors: Lee YR, Kweon SH, Kwon KB, Park JW, Yoon TR, Park BH
      Biochem. Biophys. Res. Commun., 2009;378(1):90-4.
      Species: Human
      Sample Type: Cell Culture Supernates
    4. IL-8 as antibody therapeutic target in inflammatory diseases: reduction of clinical activity in palmoplantar pustulosis.
      Authors: Skov L, Beurskens FJ, Zachariae CO, Reitamo S, Teeling J, Satijn D, Knudsen KM, Boot EP, Hudson D, Baadsgaard O, Parren PW, van de Winkel JG
      J. Immunol., 2008;181(1):669-79.
      Species: Human
      Sample Type: palmoplantar pustulosis wash fluid
    5. Coevolution of prostate cancer and bone stroma in three-dimensional coculture: implications for cancer growth and metastasis.
      Authors: Sung SY, Hsieh CL, Law A, Zhau HE, Pathak S, Multani AS, Lim S, Coleman IM, Wu LC, Figg WD, Dahut WL, Nelson P, Lee JK, Amin MB, Lyles R, Johnstone PA, Marshall FF, Chung LW
      Cancer Res., 2008;68(23):9996-10003.
      Species: Human
      Sample Type: Serum
    6. Intestinal epithelial cell up-regulation of LY6 molecules during colitis results in enhanced chemokine secretion.
      Authors: Flanagan K, Modrusan Z, Cornelius J, Chavali A, Kasman I, Komuves L, Mo L, Diehl L
      J. Immunol., 2008;180(6):3874-81.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    7. Guggulsterone blocks IL-1beta-mediated inflammatory responses by suppressing NF-kappaB activation in fibroblast-like synoviocytes.
      Authors: Lee YR, Lee JH, Noh EM, Kim EK, Song MY, Jung WS, Park SJ, Kim JS, Park JW, Kwon KB, Park BH
      Life Sci., 2008;82(23):1203-9.
      Species: Human
      Sample Type: Cell Culture Supernates
    8. CT20126, a novel immunosuppressant, prevents collagen-induced arthritis through the down-regulation of inflammatory gene expression by inhibiting NF-kappaB activation.
      Authors: Lee SJ, Nam WD, Na HJ, Cho YL, Ha KS, Hwang JY, Lee H, Kim SO, Kwon YG, Kim YM
      Biochem. Pharmacol., 2008;76(1):79-90.
      Species: Human
      Sample Type: Cell Culture Supernates
    9. Th1/Th2 cytokines reciprocally regulate in vitro pulmonary angiogenesis via CXC chemokine synthesis.
      Authors: Matsuda A, Fukuda S, Matsumoto K, Saito H
      Am. J. Respir. Cell Mol. Biol., 2007;38(2):168-75.
      Species: Human
      Sample Type: Cell Culture Supernates
    10. Expression and gene polymorphisms of the chemokine CXCL5 in colorectal cancer patients.
      Authors: Dimberg J, Dienus O, Lofgren S, Hugander A, Wagsater D
      Int. J. Oncol., 2007;31(1):97-102.
      Species: Human
      Sample Type: Plasma
    11. L5, the most electronegative subfraction of plasma LDL, induces endothelial vascular cell adhesion molecule 1 and CXC chemokines, which mediate mononuclear leukocyte adhesion.
      Authors: Abe Y, Fornage M, Yang CY, Bui-Thanh NA, Wise V, Chen HH, Rangaraj G, Ballantyne CM
      Atherosclerosis, 2007;192(1):56-66.
      Species: Human
      Sample Type: Cell Culture Supernates
    12. Cooperative effects of rhinovirus and TNF-{alpha} on airway epithelial cell chemokine expression.
      Authors: Newcomb DC, Sajjan US, Nagarkar DR, Goldsmith AM, Bentley JK, Hershenson MB
      Am. J. Physiol. Lung Cell Mol. Physiol., 2007;293(4):L1021-8.
      Species: Human
      Sample Type: Cell Culture Supernates
    13. Crucial role of the protein C pathway in governing microvascular inflammation in inflammatory bowel disease.
      Authors: Scaldaferri F, Sans M, Vetrano S, Graziani C, De Cristofaro R, Gerlitz B, Repici A, Arena V, Malesci A, Panes J, Grinnell BW, Danese S
      J. Clin. Invest., 2007;117(7):1951-60.
      Species: Human
      Sample Type: Cell Culture Supernates
    14. Different angiogenic activity in pulmonary sarcoidosis and idiopathic pulmonary fibrosis.
      Authors: Antoniou KM, Tzouvelekis A, Alexandrakis MG, Sfiridaki K, Tsiligianni I, Rachiotis G, Tzanakis N, Bouros D, Milic-Emili J, Siafakas NM
      Chest, 2006;130(4):982-8.
      Species: Human
      Sample Type: BALF
    15. IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
      Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
      J. Immunol., 2005;175(9):6177-89.
      Species: Human
      Sample Type: Cell Culture Supernates
    16. CXC-chemokine stimulation of neutrophils correlates with plasma levels of myeloperoxidase and lactoferrin and contributes to clinical outcome after pediatric cardiac surgery.
      Authors: Gessler P, Pretre R, Hohl V, Rousson V, Fischer J, Dahinden C
      Shock, 2004;22(6):513-20.
      Species: Human
      Sample Type: Plasma
    17. Peritoneal fluid concentrations of epithelial neutrophil-activating peptide-78 correlate with the severity of endometriosis.
      Authors: Suzumori N, Katano K, Suzumori K
      Fertil. Steril., 2004;81(2):305-8.
      Species: Human
      Sample Type: Peritoneal Fluid
    18. ELR+ CXC chemokines in human milk.
      Authors: Maheshwari A, Christensen RD, Calhoun DA
      Cytokine, 2003;24(3):91-102.
      Species: Human
      Sample Type: Serum
    19. Evasion from NK cell immunity by MHC class I chain-related molecules expressing colon adenocarcinoma.
      Authors: Doubrovina ES, Doubrovin MM, Vider E, Sisson RB, O&amp;amp;apos;Reilly RJ, Dupont B, Vyas YM
      J. Immunol., 2003;171(12):6891-9.
      Species: Human
      Sample Type: Serum
    20. TNFalpha-induced macrophage chemokine secretion is more dependent on NF-kappaB expression than lipopolysaccharides-induced macrophage chemokine secretion.
      Authors: Ciesielski CJ, Andreakos E, Foxwell BM, Feldmann M
      Eur. J. Immunol., 2002;32(7):2037-45.
      Species: Human
      Sample Type: Cell Culture Supernates
    Expand to show all
    ELISA Controls
    Description Application Cat# Citations Images  

    Quantikine Immunoassay Control Group 7

    Ctrl QC22
    View Sizes & Prices
    Catalog# Size Price Stock Quantity
    Loading Information....
    Supplemental ELISA Products
    Description Application Cat# Citations Images  

    Quantikine Wash Buffer 1

    ELISA WA126 5
    View Sizes & Prices
    Catalog# Size Price Stock Quantity
    Loading Information....

    Have you used Human CXCL5/ENA-78 Quantikine ELISA Kit?

    Submit a review and receive a $25 amazon gift card if you include an image - $10 Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

    Order Details

    Contact Information

    Certificate of Analysis Lookup

    The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided

    Certificate of Analysis Lookup

    Research Areas of Interest
    R&D Systems Guarantee

    Customers Who Viewed
    This Item Also Viewed


    Customer Information