Detection of CXCR4 in Jurkat Human Cell Line by Flow Cytometry.
Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human CXCR4 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB172R, filled histogram) or isotype control antibody(Catalog # IC0041R, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
CXCR4 is a G-protein-linked seven transmembrane spanning receptor that binds stromal cell-derived factor-1 (SDF-1). CXCR4 acts as a co-factor for T-cell tropic HIV-1 and -2 viral entry into cells. While primarily a membrane protein, CXCR4 undergoes trafficking and internalization in response to stimulation with phorbol esters and ligand (1). Cytoplasmic and nuclear localization of CXCR4 has been observed in colorectal and renal carcinomas (2,3) and it has been used as the basis of prognosis and metastatic state (3,4,5).
Orsini, M.J. et al. (1999) J. Biol. Chem. 274:31076.
Zagzag, D. et al. (2005) Cancer Res. 65:6178.
Speetjens, F.M. et al. (2009) Cancer Microenvironment 2:1.
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