Human CXCR4 Alexa Fluor® 647-conjugated Antibody

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  • Species Reactivity
    Human
  • Specificity
    Detects human CXCR4. It will also react with cells expressing feline CXCR4 but not rat CXCR4.
  • Source
    Monoclonal Mouse IgG2B Clone # 44716
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    3T3 cells transfected with human CXCR4
  • Formulation
    Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 647
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25-1 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of CXCR4 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human CXCR4 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB172R, filled histogram) or isotype control antibody(Catalog # IC0041R, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CXCR4
CXCR4 is a G-protein-linked seven transmembrane spanning receptor that binds stromal cell-derived factor-1 (SDF-1). CXCR4 acts as a co-factor for T-cell tropic HIV-1 and -2 viral entry into cells. While primarily a membrane protein, CXCR4 undergoes trafficking and internalization in response to stimulation with phorbol esters and ligand (1). Cytoplasmic and nuclear localization of CXCR4 has been observed in colorectal and renal carcinomas (2,3) and it has been used as the basis of prognosis and metastatic state (3,4,5).
  • References:
    1. Orsini, M.J. et al. (1999) J. Biol. Chem. 274:31076.
    2. Zagzag, D. et al. (2005) Cancer Res. 65:6178.
    3. Speetjens, F.M. et al. (2009) Cancer Microenvironment 2:1.
    4. Wang, L. et al. (2009) Oncology Reports 22:1333.
    5. Amara, S. et al. (2015) Cancer Biomark. 15:869.
  • Entrez Gene IDs:
    7852 (Human); 12767 (Mouse); 60628 (Rat); 483900 (Canine); 493676 (Feline)
  • Alternate Names:
    CD184; chemokine (C-X-C motif) receptor 4; chemokine (C-X-C motif), receptor 4 (fusin); C-X-C chemokine receptor type 4; CXCR4; CXC-R4; CXCR-4; D2S201ESDF-1 receptor; FB22; Fusin; HM89NPYRL; HSY3RR; LAP3; LCR1; LESTRCD184 antigen; Leukocyte-derived seven transmembrane domain receptor; leukocyte-derived seven-transmembrane-domain receptor; lipopolysaccharide-associated protein 3; neuropeptide Y receptor Y3; NPY3RWHIM; NPYR; NPYY3R; seven transmembrane helix receptor; seven-transmembrane-segment receptor, spleen; Stromal cell-derived factor 1 receptor
Related Research Areas

FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Alexa Fluor® 647-conjugated Isotype Control

Ctrl IC0041R
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 10
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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Average Rating: 5 (Based on 1 review)

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Read what people are saying who have used Human CXCR4 Alexa Fluor® 647-conjugated Antibody.

We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow Cytometry.

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Excellent
 Flow Human Anonymous 03/23/2018
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Summary

Application Flow Cytometry
Sample Tested Blood mononuclear cells (PBMCs)
Species Human

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