Human CXCR4 Antibody

Catalog # Availability Size / Price Qty
MAB172-100
MAB172-SP
Detection of CXCR4 in Jurkat Human Cell Line by Flow Cytometry.
8 Images
Product Details
Citations (87)
FAQs
Supplemental Products
Reviews (4)

Human CXCR4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CXCR4. It will also react with cells expressing feline CXCR4 but not rat CXCR4.
Source
Monoclonal Mouse IgG2B Clone # 44716
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
3T3 cells transfected with human CXCR4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Measured by its ability to neutralize CXCL12/SDF‑1 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR4. The Neutralization Dose (ND50) is typically 2.5-12 µg/mL in the presence of 1 ng/mL Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CXCR4 antibody in Jurkat Human Cell Line antibody by Flow Cytometry. View Larger

Detection of CXCR4 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human CXCR4 Monoclonal Antibody (Catalog # MAB172, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry CXCR4 antibody in Human Lymph Node by Immunohistochemistry (IHC-P). View Larger

CXCR4 in Human Lymph Node. CXCR4 was detected in immersion fixed paraffin-embedded sections of human lymph node using 15 µg/mL Human CXCR4 Monoclonal Antibody (Catalog # MAB172) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry CXCR4 antibody in Human Spleen by Immunohistochemistry (IHC-P). View Larger

CXCR4 in Human Spleen. CXCR4 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human CXCR4 Monoclonal Antibody (Catalog # MAB172) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Neutralization Chemotaxis Induced by CXCL12/SDF‑1 alpha  and Neutralization by Human CXCR4 Antibody. View Larger

Chemotaxis Induced by CXCL12/SDF‑1 alpha and Neutralization by Human CXCR4 Antibody. Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF-1a (Catalog # 350-NS) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF-1a (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCR4 Monoclonal Antibody (Catalog # MAB172). The ND50 is typically 2.5-12 µg/mL.

Flow Cytometry Detection of Human CXCR4 by Flow Cytometry View Larger

Detection of Human CXCR4 by Flow Cytometry CXCR4 neutralization leads to attenuation of the CD44+/CD133+ prostate progenitor population.(A) DU145 cells were plated in 96-well low-attachment plates at 100 cells per well (5 replicates) and the spheres were grown in serum-free, EBM medium with supplements. The antibodies were replenished daily. Cells were imaged with an Acumen eX3 microplate cytometer and spheres were detected using image analysis software. The sphere size was measured by GFP intensity. The spheres were discriminated from cell debris using a Gaussian filter. The spheres included in the analysis are outlined in red and indicated by arrows. Representative data from one of two independent experiments is shown; *- p value<0.05. (B) Flow cytometry analysis revealed attenuation of CD44+/CD133+ population in DU145 cells treated with 10 µg/ml neutralizing anti-CXCR4 (mouse monoclonal IgG, clone 44716, R&D Systems) or 10 µg/ml control antibody (mouse IgG isotype control, Lifespan Bioscience Inc.) for 5 days. The cell were grown in medium supplemented with 2% FBS. Culture medium was refreshed every second day; *- p value<0.05. (C) Western blot analysis of DU145 cells treated with 10 µg/ml neutralizing anti-CXCR4 antibody for 5 days demonstrated downregulation of the PI3K/AKT pathway activation compared to the cells treated with 10 µg/ml control antibody. The cell were grown in medium supplemented with 2% FBS. Culture medium was refreshed every second day. (D) Preincubation of prostate cancer cells with neutralizing anti-CXCR4 antibody significantly delays tumor growth. 5×105 DU145 cells pretreated with neutralizing anti-CXCR4 or control antibody for 5 days were embedded in BD matrigel and injected s.c. into NOD/SCID mice.*- p value<0.01. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0031226), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Human CXCR4 by Immunohistochemistry View Larger

Detection of Human CXCR4 by Immunohistochemistry CXCR4 and CD133 are highly expressed in ovarian cancer patients. (A) Representative panel of immunohistochemistry for CD133 and CXCR4 expression (Magnification 200X; insert 400X). Non-homogeneous and focal cytoplasmic and membranous CD133 expression rated as negative (I), focally low (1–10% cancer cells) (II), focally high CD133 expression (>10% stained cancer cells) (III). Extensive homogeneous cytoplasmic and membranous, CXCR4 staining was reported rated as negative (IV) moderate (<50% of cancer cells) (V) and high expression (>50% of cancer cells) (VI). (B) QPCR analysis of CXCR4 and CD133 in freshly resected ovarian tumors and corresponding normal ovarian tissue. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/srep10357), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Human CXCR4 by Immunohistochemistry View Larger

Detection of Human CXCR4 by Immunohistochemistry Immunohistochemical staining for MIF and CXCR4 in human esophageal carcinoma. Our data showed low expression levels of MIF (a) and CXCR4 (c) (X 400) and high expression levels of MIF (b) and CXCR4 (d), compared with the negative control (e) (X 400), in tumor tissues from patients with ESCC. The arrows point to the positive staining of tumor cells or TILs. Image collected and cropped by CiteAb from the following publication (https://translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-11-60), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Human CXCR4 by Immunohistochemistry View Larger

Detection of Human CXCR4 by Immunohistochemistry Immunohistochemical staining for MIF and CXCR4 in human esophageal carcinoma. Our data showed low expression levels of MIF (a) and CXCR4 (c) (X 400) and high expression levels of MIF (b) and CXCR4 (d), compared with the negative control (e) (X 400), in tumor tissues from patients with ESCC. The arrows point to the positive staining of tumor cells or TILs. Image collected and cropped by CiteAb from the following publication (https://translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-11-60), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCR4

CXCR4, also known as CD184, is a G-protein-linked seven transmembrane spanning receptor that binds stromal cell-derived factor-1 (SDF-1). CXCR4 acts as a co-factor for T-cell tropic HIV-1 and -2 viral entry into cells. While primarily a membrane protein, CXCR4 undergoes trafficking and internalization in response to stimulation with phorbol esters and ligand (1). Cytoplasmic and nuclear localization of CXCR4 has been observed in colorectal and renal carcinomas (2,3) and it has been used as the basis of prognosis and metastatic state (3,4,5).

References
  1. Orsini, M.J. et al. (1999) J. Biol. Chem. 274:31076.
  2. Zagzag, D. et al. (2005) Cancer Res. 65:6178.
  3. Speetjens, F.M. et al. (2009) Cancer Microenvironment 2:1.
  4. Wang, L. et al. (2009) Oncology Reports 22:1333.
  5. Amara, S. et al. (2015) Cancer Biomark. 15:869.
Long Name
C-X-C Motif Chemokine Receptor 4
Entrez Gene IDs
7852 (Human); 12767 (Mouse); 60628 (Rat); 483900 (Canine); 493676 (Feline)
Alternate Names
CD184; chemokine (C-X-C motif) receptor 4; chemokine (C-X-C motif), receptor 4 (fusin); C-X-C chemokine receptor type 4; CXCR4; CXC-R4; CXCR-4; D2S201E; D2S201ESDF-1 receptor; FB22; Fusin; HM89; HM89NPYRL; HSY3RR; LAP3; LAP-3; LCR1; LESTR; LESTRCD184 antigen; Leukocyte-derived seven transmembrane domain receptor; leukocyte-derived seven-transmembrane-domain receptor; lipopolysaccharide-associated protein 3; neuropeptide Y receptor Y3; NPY3R; NPY3RWHIM; NPYR; NPYRL; NPYY3R; pCXCR4; seven transmembrane helix receptor; seven-transmembrane-segment receptor, spleen; Stromal cell-derived factor 1 receptor; WHIMS

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Citations for Human CXCR4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

87 Citations: Showing 1 - 10
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  1. Recruitment of mesenchymal stem cells by stromal cell-derived factor 1 alpha in pulp cells from deciduous teeth
    Authors: YUKI AKAZAWA, TOMOKAZU HASEGAWA, YOSHITAKA YOSHIMURA, NAOYUKI CHOSA, TAKEYOSHI ASAKAWA, KIMIKO UEDA et al.
    International Journal of Molecular Medicine
  2. A small molecule-based strategy for endothelial differentiation and three-dimensional morphogenesis from human embryonic stem cells
    Authors: Yijie Geng, Bradley Feng
    Heliyon
  3. Single-cell dissection of Merkel cell carcinoma heterogeneity unveils transcriptomic plasticity and therapeutic vulnerabilities
    Authors: Bhaba K. Das, Aarthi Kannan, Graham J. Velasco, Mikaela D. Kunika, Nils Lambrecht, Quy Nguyen et al.
    Cell Reports Medicine
  4. CXCR4 signaling at the ovine fetal–maternal interface regulates vascularization, CD34+ cell presence, and autophagy in the endometrium†
    Authors: Cheyenne L Runyan, Stacia Z McIntosh, Marlie M Maestas, Kelsey E Quinn, Ben P Boren, Ryan L Ashley
    Biology of Reproduction
  5. CXCR4 expression affects overall survival of HCC patients whereas CXCR7 expression does not
    Authors: Maria Neve Polimeno, Caterina Ierano, Crescenzo D'Alterio, Nunzia Simona Losito, Maria Napolitano, Luigi Portella et al.
    Cellular & Molecular Immunology
  6. CXCR4 suppression attenuates EGFRvIII-mediated invasion and induces p38 MAPK-dependent protein trafficking and degradation of EGFRvIII in breast cancer cells
    Authors: Massod Rahimi, Theodore A. Toth, Careen K. Tang
    Cancer Letters
  7. A meta-analysis of CXCL12 expression for cancer prognosis
    Authors: Harsh Samarendra, Keaton Jones, Tatjana Petrinic, Michael A Silva, Srikanth Reddy, Zahir Soonawalla et al.
    British Journal of Cancer
  8. Hepatic stellate cells express functional CXCR4: role in stromal cell-derived factor-1alpha-mediated stellate cell activation
    Authors: Feng Hong, Ana Tuyama, Ting Fang Lee, Johnny Loke, Ritu Agarwal, Xin Cheng et al.
    Hepatology
  9. Estrogen-dependent regulation of human uterine natural killer cells promotes vascular remodelling via secretion of CCL2
    Authors: D.A. Gibson, E. Greaves, H.O.D. Critchley, P.T.K. Saunders
    Human Reproduction
  10. In Vivo Invasion of Head and Neck Squamous Cell Carcinoma Cells Does Not Require Macrophages
    Authors: Tatiana Smirnova, Alfred Adomako, Joseph Locker, Nico Van Rooijen, Michael B. Prystowsky, Jeffrey E. Segall
    The American Journal of Pathology
  11. Hypoxia stimulates CXCR4 signalling in ileal carcinoids.
    Authors: Arvidsson Y, Bergstrom A, Arvidsson L et al.
    Endocr Relat Cancer
  12. Senescent tumor cells lead the collective invasion in thyroid cancer
    Authors: Kim YH, Choi YW, Lee J et al.
    Nat Commun.
  13. Identification of a distinct population of CD133(+)CXCR4(+) cancer stem cells in ovarian cancer
    Authors: Michele Cioffi, Crescenzo D’Alterio, Rosalba Camerlingo, Virginia Tirino, Claudia Consales, Anna Riccio et al.
    Scientific Reports
  14. 99mTc-CXCR4-L for Imaging of the Chemokine-4 Receptor Associated with Brain Tumor Invasiveness: Biokinetics, Radiation Dosimetry, and Proof of Concept in Humans
    Authors: Paola Vallejo-Armenta, Clara Santos-Cuevas, Juan Soto-Andonaegui, Rosa M. Villanueva-Pérez, Jorge I. González-Díaz, Francisco O. García-Pérez et al.
    Contrast Media & Molecular Imaging
  15. Phosphatidylcholine-specific phospholipase C inhibition down- regulates CXCR4 expression and interferes with proliferation, invasion and glycolysis in glioma cells
    Authors: L Mercurio, S Cecchetti, A Ricci, A Pacella, G Cigliana, G Bozzuto, F Podo, E Iorio, G Carpinelli
    PLoS ONE, 2017-04-19;12(4):e0176108.
  16. The chemokine, CXCL12, is an independent predictor of poor survival in ovarian cancer
    Authors: A Popple, L G Durrant, I Spendlove, P Rolland, I V Scott, S Deen et al.
    British Journal of Cancer
  17. Chemotherapy Negates the Effect of SDF1 mRNA to Distant Metastasis and Poor Overall Survival in Breast Cancer Patients
    Authors: Kristanto Yuli Yarso, Monica Bellynda, Akhmad Azmiardi, Brian Wasita, Didik Setyo Heriyanto, Indwiani Astuti et al.
    Asian Pacific Journal of Cancer Prevention
  18. CXCR4-CXCL12-CXCR7 and PD-1/PD-L1 in Pancreatic Cancer: CXCL12 Predicts Survival of Radically Resected Patients
    Authors: C D'Alterio, A Giardino, G Scognamigl, G Butturini, L Portella, G Guardascio, I Frigerio, M Montella, S Gobbo, G Martignoni, V Napolitano, F De Vita, F Tatangelo, R Franco, S Scala
    Cells, 2022-10-22;11(21):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  19. Analysis of the chemotactic factors for tumor-infiltrating fibrocytes and their prognostic significances in lung cancer
    Authors: M Tobiume, A Mitsuhashi, A Saijo, H Ogino, T Afroj, H Ogawa, H Goto, S Sato, A Abe, K Haji, R Ozaki, H Takizawa, Y Nishioka
    Oncology Letters, 2022-09-30;24(5):417.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  20. Biological Role of Tumor/Stromal CXCR4-CXCL12-CXCR7 in MITO16A/MaNGO-OV2 Advanced Ovarian Cancer Patients
    Authors: C D'Alterio, A Spina, L Arenare, P Chiodini, M Napolitano, F Galdiero, L Portella, V Simeon, S Signoriell, F Raspaglies, D Lorusso, C Pisano, N Colombo, GF Zannoni, NS Losito, R De Cecio, G Scognamigl, D Califano, D Russo, V Tuninetti, MC Piccirillo, P Gargiulo, F Perrone, S Pignata, S Scala
    Cancers, 2022-04-06;14(7):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  21. Combination of IDO1high and CCL19low expression in the tumor tissue reduces survival in HPV positive cervical cancer
    Authors: H Jayakumar, A Seetharama, S Sunder Sin, H Dhandapani, J Subramani, S Ganeshraja, R Thangaraja, P Ramanathan
    Journal of reproductive immunology, 2021-11-23;149(0):103454.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  22. Role of CCR3 in respiratory syncytial virus infection of airway epithelial cells.
    Authors: Wellemans V, Benhassou H, Fuselier E, Bellesort F, Dury S, Lebargy F, Dormoy V, Fichel C, Naour R, Gounni A, Lamkhioued B
    iScience, 2021-11-14;24(12):103433.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. Mechanisms of HIV-1 evasion to the antiviral activity of chemokine CXCL12 indicate potential links with pathogenesis
    Authors: M Armani-Tou, Z Zhou, R Gasser, I Staropoli, V Cantaloube, Y Benureau, J Garcia-Per, M Pérez-Olme, V Lorin, B Puissant-L, L Assoumou, C Delaugerre, JD Lelièvre, Y Lévy, H Mouquet, G Martin-Blo, J Alcami, F Arenzana-S, J Izopet, P Colin, B Lagane
    PloS Pathogens, 2021-04-19;17(4):e1009526.
    Species: Human
    Sample Types: Whole Cells
    Applications: Binding Assay
  24. Gene expression and functional deficits underlie TREM2-knockout microglia responses in human models of Alzheimer's disease
    Authors: A McQuade, YJ Kang, J Hasselmann, A Jairaman, A Sotelo, M Coburn, SK Shabestari, JP Chadarevia, G Fote, CH Tu, E Danhash, J Silva, E Martinez, C Cotman, GA Prieto, LM Thompson, JS Steffan, I Smith, H Davtyan, M Cahalan, H Cho, M Blurton-Jo
    Nat Commun, 2020-10-23;11(1):5370.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  25. Soluble Compounds Released by Hypoxic Stroma Confer Invasive Properties to Pancreatic Ductal Adenocarcinoma
    Authors: D Liu, A Steins, R Klaassen, AP van der Za, RJ Bennink, G van Tienho, MG Besselink, MF Bijlsma, HWM van Laarho
    Biomedicines, 2020-10-22;8(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  26. Involvement of CXCL12/CXCR4 in the motility of human first-trimester endometrial epithelial cells through an autocrine mechanism by activating PI3K/AKT signaling
    Authors: J Zheng, D Qu, C Wang, L Ding, W Zhou
    BMC Pregnancy Childbirth, 2020-02-10;20(1):87.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC, Neutralization
  27. High CXCR4 Expression Predicts a Poor Prognosis in Resected Lung Adenosquamous Carcinoma
    Authors: Q Zhu, R Luo, J Gu, Y Hou, Z Chen, F Xu, L Wang, W Mao, C Lu, D Ge
    J Cancer, 2020-01-01;11(4):810-818.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  28. Clinicopathologic determinants of pathologic treatment response in neoadjuvant treated rectal adenocarcinoma
    Authors: I González, PS Bauer, WC Chapman, Z Alipour, R Rais, J Liu, D Chatterjee
    Ann Diagn Pathol, 2019-12-14;45(0):151452.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  29. Chemokine Receptor Expression Pattern Correlates to Progression of Conjunctival Melanocytic Lesions
    Authors: JA van Ipenbu, NE de Waard, NC Naus, MJ Jager, D Paridaens, RM Verdijk
    Invest. Ophthalmol. Vis. Sci., 2019-07-01;60(8):2950-2957.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  30. Clinical impact of different exosomes' protein expression in pancreatic ductal carcinoma patients treated with standard first line palliative chemotherapy
    Authors: R Giampieri, F Piva, G Occhipinti, A Bittoni, A Righetti, S Pagliarett, A Murrone, F Bianchi, C Amantini, M Giulietti, G Ricci, G Principato, G Santoni, R Berardi, S Cascinu
    PLoS ONE, 2019-05-02;14(5):e0215990.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Detection
  31. Breast Cancer: An Examination of the Potential of ACKR3 to Modify the Response of CXCR4 to CXCL12
    Authors: I Del Molino, GC Wilkins, A Meeson, S Ali, JA Kirby
    Int J Mol Sci, 2018-11-14;19(11):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  32. Senescent fibroblasts drive ageing pigmentation: ?A potential therapeutic target for senile lentigo
    Authors: JE Yoon, Y Kim, S Kwon, M Kim, YH Kim, JH Kim, TJ Park, HY Kang
    Theranostics, 2018-09-09;8(17):4620-4632.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-P
  33. Delayed and repeated intranasal delivery of bone marrow stromal cells increases regeneration and functional recovery after ischemic stroke in mice
    Authors: MJ Chau, TC Deveau, X Gu, YS Kim, Y Xu, SP Yu, L Wei
    BMC Neurosci, 2018-04-12;19(1):20.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  34. Tumor Microenvironment in Functional Adrenocortical Adenomas: Immune Cell Infiltration in Cortisol-producing Adrenocortical Adenoma
    Authors: Y Kitawaki, Y Nakamura, F Kubota-Nak, Y Yamazaki, Y Miki, S Hata, K Ise, K Kikuchi, R Morimoto, F Satoh, H Sasano
    Hum. Pathol., 2018-03-27;0(0):.
    Species: Human
    Sample Types: WholeTissue
    Applications: IHC-P
  35. Expression of CXC-motif-chemokine 12 and the receptor C-X-C receptor 4 in glioma and theeffect on peritumoral brain edema
    Authors: W Tang, Y Chen, X Wang, Y Chen, J Zhang, Z Lin
    Oncol Lett, 2017-12-08;15(2):2501-2507.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  36. Mechanisms by which CXCR4/CXCL12 cause metastatic behavior in pancreatic cancer
    Authors: J Zhang, C Liu, X Mo, H Shi, S Li
    Oncol Lett, 2017-12-05;15(2):1771-1776.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  37. Cell-based therapy using miR-302-367 expressing cells represses glioblastoma growth
    Authors: M Fareh, F Almairac, L Turchi, F Burel-Vand, P Paquis, D Fontaine, S Lacas-Gerv, MP Junier, H Chneiweiss, T Virolle
    Cell Death Dis, 2017-03-30;8(3):e2713.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  38. Cathepsin K cleavage of SDF-1? inhibits its chemotactic activity towards glioblastoma stem-like cells
    Authors: Vashendriya V V Hira
    Biochim. Biophys. Acta, 2016-12-28;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  39. The Glycosylphosphatidylinositol-Anchored Variable Region of Llama Heavy Chain-Only Antibody JM4 Efficiently Blocks both Cell-Free and T Cell-T Cell Transmission of Human Immunodeficiency Virus Type 1
    Authors: L Liu, W Wang, J Matz, C Ye, L Bracq, J Delon, JT Kimata, Z Chen, S Benichou, P Zhou
    J. Virol., 2016-11-14;90(23):10642-10659.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  40. Signal transmission through the CXC chemokine receptor 4 (CXCR4) transmembrane helices
    Proc Natl Acad Sci USA, 2016-08-19;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-Fr
  41. Targeting CXCR4 by a selective peptide antagonist modulates tumor microenvironment and microglia reactivity in a human glioblastoma model.
    Authors: Mercurio L, Ajmone-Cat M, Cecchetti S, Ricci A, Bozzuto G, Molinari A, Manni I, Pollo B, Scala S, Carpinelli G, Minghetti L
    J Exp Clin Cancer Res, 2016-03-25;35(0):55.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  42. Simultaneous Activation of Induced Heterodimerization between CXCR4 Chemokine Receptor and Cannabinoid Receptor 2 (CB2) Reveals a Mechanism for Regulation of Tumor Progression
    Authors: CJ Coke, KA Scarlett, MA Chetram, KJ Jones, BJ Sandifer, AS Davis, AI Marcus, CV Hinton
    J. Biol. Chem., 2016-02-03;291(19):9991-10005.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  43. Identification and Characterization of CXCR4-Positive Gastric Cancer Stem Cells.
    Authors: Fujita T, Chiwaki F, Takahashi R, Aoyagi K, Yanagihara K, Nishimura T, Tamaoki M, Komatsu M, Komatsuzaki R, Matsusaki K, Ichikawa H, Sakamoto H, Yamada Y, Fukagawa T, Katai H, Konno H, Ochiya T, Yoshida T, Sasaki H
    PLoS ONE, 2015-06-25;10(6):e0130808.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  44. The long pentraxin PTX3 promotes fibrocyte differentiation.
    Authors: Pilling D, Cox N, Vakil V, Verbeek J, Gomer R
    PLoS ONE, 2015-03-16;10(3):e0119709.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-Fr
  45. CXCL16 and CXCR6 are coexpressed in human lung cancer in vivo and mediate the invasion of lung cancer cell lines in vitro.
    Authors: Hu, Weidong, Liu, Yue, Zhou, Wenhui, Si, Lianlian, Ren, Liang
    PLoS ONE, 2014-06-04;9(6):e99056.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-P
  46. Prevailing role of contact guidance in intrastromal T-cell trapping in human pancreatic cancer.
    Authors: Hartmann N, Giese N, Giese T, Poschke I, Offringa R, Werner J, Ryschich E
    Clin Cancer Res, 2014-04-24;20(13):3422-33.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  47. Vasoreparative dysfunction of CD34+ cells in diabetic individuals involves hypoxic desensitization and impaired autocrine/paracrine mechanisms.
    Authors: Jarajapu Y, Hazra S, Segal M, Li Calzi S, Jadhao C, Qian K, Mitter S, Raizada M, Boulton M, Grant M
    PLoS ONE, 2014-04-08;9(4):e93965.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  48. An important role of the SDF-1/CXCR4 axis in chronic skin inflammation.
    Authors: Zgraggen, Silvana, Huggenberger, Reto, Kerl, Katrin, Detmar, Michael
    PLoS ONE, 2014-04-02;9(4):e93665.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  49. Lymphatic specific disruption in the fine structure of heparan sulfate inhibits dendritic cell traffic and functional T cell responses in the lymph node.
    Authors: Yin X, Johns S, Kim D, Mikulski Z, Salanga C, Handel T, Macal M, Zuniga E, Fuster M
    J Immunol, 2014-02-03;192(5):2133-42.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  50. PCaAnalyser: a 2D-image analysis based module for effective determination of prostate cancer progression in 3D culture.
    Authors: Hoque, Md Tamji, Windus, Louisa C, Lovitt, Carrie J, Avery, Vicky M
    PLoS ONE, 2013-11-20;8(11):e79865.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  51. Expression of the chemokine receptor CXCR7 in CXCR4-expressing human 143B osteosarcoma cells enhances lung metastasis of intratibial xenografts in SCID mice.
    Authors: Brennecke P, Arlt M, Muff R, Campanile C, Gvozdenovic A, Husmann K, Holzwarth N, Cameroni E, Ehrensperger F, Thelen M, Born W, Fuchs B
    PLoS ONE, 2013-09-10;8(9):e74045.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  52. CXC chemokine receptor 4 is essential for maintenance of renal cell carcinoma-initiating cells and predicts metastasis.
    Authors: Gassenmaier M, Chen D, Buchner A, Henkel L, Schiemann M, Mack B, Schendel D, Zimmermann W, Pohla H
    Stem Cells, 2013-08-01;31(8):1467-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-P
  53. Inhibition of CXCR4-CXCL12 chemotaxis in melanoma by AMD11070.
    Authors: O'Boyle, G, Swidenbank, I, Marshall, H, Barker, C E, Armstrong, J, White, S A, Fricker, S P, Plummer, R, Wright, M, Lovat, P E
    Br J Cancer, 2013-03-28;108(8):1634-40.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  54. The expressions of MIF and CXCR4 protein in tumor microenvironment are adverse prognostic factors in patients with esophageal squamous cell carcinoma.
    Authors: Zhang, Lin, Ye, Shu-Biao, Ma, Gang, Tang, Xiao-Fen, Chen, Shi-Ping, He, Jia, Liu, Wan-Li, Xie, Dan, Zeng, Yi-Xin, Li, Jiang
    J Transl Med, 2013-03-08;11(0):60.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  55. The EGFR ligands amphiregulin and heparin-binding egf-like growth factor promote peritoneal carcinomatosis in CXCR4-expressing gastric cancer.
    Authors: Yasumoto K, Yamada T, Kawashima A, Wang W, Li Q, Donev IS, Tacheuchi S, Mouri H, Yamashita K, Ohtsubo K, Yano S
    Clin. Cancer Res., 2011-04-11;17(11):3619-30.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  56. Intratumoral induction of CD103 triggers tumor-specific CTL function and CCR5-dependent T-cell retention.
    Authors: Franciszkiewicz K, Le Floc'h A, Jalil A, Vigant F, Robert T, Vergnon I, Mackiewicz A, Benihoud K, Validire P, Chouaib S, Combadiere C, Mami-Chouaib F
    Cancer Res., 2009-07-28;69(15):6249-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  57. Alternative implication of CXCR4 in JAK2/STAT3 activation in small cell lung cancer.
    Authors: Pfeiffer M, Hartmann TN, Leick M, Catusse J, Schmitt-Graeff A, Burger M
    Br. J. Cancer, 2009-05-19;100(12):1949-56.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  58. A late requirement for Wnt and FGF signaling during activin-induced formation of foregut endoderm from mouse embryonic stem cells.
    Authors: Hansson M, Olesen DR, Peterslund JM, Engberg N, Kahn M, Winzi M, Klein T, Maddox-Hyttel P, Serup P
    Dev. Biol., 2009-04-07;330(2):286-304.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  59. Clinical and biological significance of CXCL12 and CXCR4 expression in adult testes and germ cell tumours of adults and adolescents.
    Authors: Gilbert DC, Chandler I, McIntyre A, Goddard NC, Gabe R, Huddart RA, Shipley J
    J. Pathol., 2009-01-01;217(1):94-102.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-P
  60. Regulation of CXCR4 by the Notch ligand delta-like 4 in endothelial cells.
    Authors: Williams CK, Segarra M, Sierra Mde L, Sainson RC, Tosato G, Harris AL
    Cancer Res., 2008-03-15;68(6):1889-95.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  61. Dynamic stromal-epithelial interactions during progression of MCF10DCIS.com xenografts.
    Authors: Tait LR, Pauley RJ, Santner SJ, Heppner GH, Heng HH, Rak JW, Miller FR
    Int. J. Cancer, 2007-05-15;120(10):2127-34.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  62. Cutting edge: nonproliferating mature immune cells form a novel type of organized lymphoid structure in idiopathic pulmonary fibrosis.
    Authors: Marchal-Somme J, Uzunhan Y, Marchand-Adam S, Valeyre D, Soumelis V, Crestani B, Soler P
    J. Immunol., 2006-05-15;176(10):5735-9.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  63. Importance of recruitment of bone marrow-derived CXCR4+ cells in post-infarct cardiac repair mediated by G-CSF.
    Authors: Misao Y, Takemura G, Arai M, Ohno T, Onogi H, Takahashi T, Minatoguchi S, Fujiwara T, Fujiwara H
    Cardiovasc. Res., 2006-05-09;71(3):455-65.
    Species: Rabbit
    Sample Types: Whole Tissue
    Applications: IHC-P
  64. Human melanoma metastases express functional CXCR4.
    Authors: Scala S, Giuliano P, Ascierto PA, Ierano C, Franco R, Napolitano M, Ottaiano A, Lombardi ML, Luongo M, Simeone E, Castiglia D, Mauro F, De Michele I, Calemma R, Botti G, Caraco C, Nicoletti G, Satriano RA, Castello G
    Clin. Cancer Res., 2006-04-15;12(8):2427-33.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-Fr, IHC-P
  65. Chemokine and chemokine receptor expression in paired peripheral blood mononuclear cells and synovial tissue of patients with rheumatoid arthritis, osteoarthritis, and reactive arthritis.
    Authors: Haringman JJ, Smeets TJ, Reinders-Blankert P
    Ann. Rheum. Dis., 2005-08-17;65(3):294-300.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  66. Ultraviolet radiation-induced injury, chemokines, and leukocyte recruitment: An amplification cycle triggering cutaneous lupus erythematosus.
    Authors: Meller S, Winterberg F, Gilliet M, Muller A, Lauceviciute I, Rieker J, Neumann NJ, Kubitza R, Gombert M, Bunemann E, Wiesner U, Franken-Kunkel P, Kanzler H, Dieu-Nosjean MC, Amara A, Ruzicka T, Lehmann P, Zlotnik A, Homey B
    Arthritis Rheum., 2005-05-01;52(5):1504-16.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  67. Recruitment of osteoclast precursors by stromal cell derived factor-1 (SDF-1) in giant cell tumor of bone.
    Authors: Liao TS, Yurgelun MB, Chang SS, Zhang HZ, Murakami K, Blaine TA, Parisien MV, Kim W, Winchester RJ, Lee FY
    J. Orthop. Res., 2005-01-01;23(1):203-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  68. CXCR4 antagonist inhibits stromal cell-derived factor 1-induced migration and invasion of human pancreatic cancer.
    Authors: Mori T, Doi R, Koizumi M, Toyoda E, Ito D, Kami K, Masui T, Fujimoto K, Tamamura H, Hiramatsu K, Fujii N, Imamura M
    Mol. Cancer Ther., 2004-01-01;3(1):29-37.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  69. Intracellular localization and constitutive endocytosis of CXCR4 in human CD34+ hematopoietic progenitor cells.
    Authors: Zhang Y, Foudi A, Geay JF, Berthebaud M, Buet D, Jarrier P, Jalil A, Vainchenker W, Louache F
    Stem Cells, 2004-01-01;22(6):1015-29.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  70. CXCR4 enhances adhesion of B16 tumor cells to endothelial cells in vitro and in vivo via beta(1) integrin.
    Authors: Cardones AR, Murakami T, Hwang ST
    Cancer Res., 2003-10-15;63(20):6751-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  71. CXCR4 function requires membrane cholesterol: implications for HIV infection.
    Authors: Nguyen DH, Taub D
    J. Immunol., 2002-04-15;168(8):4121-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Neutralization
  72. Unique subpopulations of CD56+ NK and NK-T peripheral blood lymphocytes identified by chemokine receptor expression repertoire.
    Authors: Campbell J, Qin S, Unutmaz D, Soler D, Murphy K, Hodge M, Wu L, Butcher E
    J Immunol, 2001-06-01;166(11):6477-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  73. Cysteine (C)-X-C Receptor 4 Undergoes Transportin 1-Dependent Nuclear Localization and Remains Functional at the Nucleus of Metastatic Prostate Cancer Cells
    Authors: Ayesha S. Don-Salu-Hewage, Siu Yuen Chan, Kathleen M. McAndrews, Mahandranauth A. Chetram, Michelle R. Dawson, Danaya A. Bethea et al.
    PLoS ONE
  74. The Chemokine Receptor CCR3 Is Potentially Involved in the Homing of Prostate Cancer Cells to Bone: Implication of Bone-Marrow Adipocytes
    Authors: Adrien Guérard, Victor Laurent, Gaëlle Fromont, David Estève, Julia Gilhodes, Edith Bonnelye et al.
    International Journal of Molecular Sciences
  75. CXCR4 Expression in Prostate Cancer Progenitor Cells
    Authors: Anna Dubrovska, Jimmy Elliott, Richard J. Salamone, Gennady D. Telegeev, Alexander E. Stakhovsky, Ihor B. Schepotin et al.
    PLoS ONE
  76. Hepatic differentiation of human pluripotent stem cells on human liver progenitor HepaRG-derived acellular matrix
    Authors: Liisa K. Kanninen, Pauliina Porola, Johanna Niklander, Melina M. Malinen, Anne Corlu, Christiane Guguen-Guillouzo et al.
    Experimental Cell Research
  77. Opposing roles of CXCR4 and CXCR7 in breast cancer metastasis
    Authors: Lorena Hernandez, Marco A O Magalhaes, Salvatore J Coniglio, John S Condeelis, Jeffrey E Segall
    Breast Cancer Research
  78. High expression of CXCR4, CXCR7 and SDF-1 predicts poor survival in renal cell carcinoma
    Authors: Linhui Wang, Wei Chen, Li Gao, Qing Yang, Bing Liu, Zhenjie Wu et al.
    World Journal of Surgical Oncology
  79. Increased oxidative stress and severe arterial remodeling induced by permanent high-flow challenge in experimental pulmonary hypertension
    Authors: Peter Dorfmüller, Marie-Camille Chaumais, Maria Giannakouli, Ingrid Durand-Gasselin, Nicolas Raymond, Elie Fadel et al.
    Respiratory Research
  80. Hypoxia increases the metastatic ability of breast cancer cells via upregulation of CXCR4
    Authors: Patricia A Cronin, Jiang H Wang, H Paul Redmond
    BMC Cancer
  81. Distinct stimulus-dependent neutrophil dynamics revealed by real-time imaging of intestinal mucosa after acute injury
    Authors: Veronica Azcutia, Matthias Kelm, Seonyoung Kim, Anny-Claude Luissint, Sven Flemming, Lisa Abernathy-Close et al.
    PNAS Nexus
  82. Synergistic Antitumor Effects of Endostar in Combination with Oxaliplatin via Inhibition of HIF and CXCR4 in the Colorectal Cell Line SW1116
    Authors: Fengyan Jin, Huifan Ji, Chunshu Jia, Ulf Brockmeier, Dirk M. Hermann, Eric Metzen et al.
    PLoS ONE
  83. Senescent Tumor Cells Build a Cytokine Shield in Colorectal Cancer
    Authors: Choi YW, Kim YH, Oh SY et al.
    Advanced Science
  84. Periprostatic adipocytes act as a driving force for prostate cancer progression in obesity
    Authors: Victor Laurent, Adrien Guérard, Catherine Mazerolles, Sophie Le Gonidec, Aurélie Toulet, Laurence Nieto et al.
    Nature Communications
  85. A novel cross-talk between CXCR4 and PI4KIII alpha in prostate cancer cells.
    Authors: Diego Sbrissa, Louie Semaan, Barani Govindarajan, Yanfeng Li, Nicholas J. Caruthers, Paul M. Stemmer et al.
    Oncogene
  86. In the ovine pituitary, CXCR4 is localized in gonadotropes and somatotropes and increases with elevated serum progesterone
    Authors: N.S. Sanchez, K.E. Quinn, A.K. Ashley, R.L. Ashley
    Domestic Animal Endocrinology
  87. EGFR variant-mediated invasion by enhanced CXCR4 expression through transcriptional and post-translational mechanisms.
    Authors: Rahimi Massod, George Jessica, Tang Careen et al.
    International Journal of Cancer

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Human CXCR4 Antibody
By Anonymous on 08/03/2021
Application: IHC Sample Tested: Ductal carcinoma,Tumor tissue Species: Human

Human CXCR4 Antibody
By Anonymous on 12/07/2020
Application: MiAr Sample Tested: EDTA Plasma Species: Human

Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma


Human CXCR4 Antibody
By Anonymous on 11/16/2018
Sample Tested: Ramos human Burkitt's lymphoma cell line,SUDHL-6 Species: Human

Cell line stained with human CXCR4 for 45 min and secondary antibody anti-mouse FITC for 45min & Isotype control and unstained cell used in experiment


Human CXCR4 Antibody
By Anonymous on 03/15/2018
Application: Flow Sample Tested: Human cell line Species: Human