Human CXCR4 PE-conjugated Antibody

(21 citations)
(7 Reviews)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human CXCR4.
  • Source
    Monoclonal Mouse IgG2A Clone # 12G5
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    CP-MAC-infected SUP-T1 human T cell lymphoblastic lymphoma line
    Accession # P61073
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CXCR4 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CXCR4 PE‑conjugated Monoclonal Antibody (Catalog # FAB170P) and Mouse Anti-Human CD19 APC‑conjugated Monoclonal Antibody (Catalog # FAB4867A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CXCR4

CXCR4 is a G-protein-linked seven transmembrane spanning receptor that binds stromal cell-derived factor-1 (SDF-1). CXCR4 acts as a co-factor for T-cell tropic HIV-1 and -2 viral entry into cells.

  • References:
    1. Endres, M.J. et al. (1996) Cell 87:745.
  • Entrez Gene IDs:
    7852 (Human); 12767 (Mouse); 60628 (Rat); 483900 (Canine); 493676 (Feline)
  • Alternate Names:
    CD184; chemokine (C-X-C motif) receptor 4; chemokine (C-X-C motif), receptor 4 (fusin); C-X-C chemokine receptor type 4; CXCR4; CXC-R4; CXCR-4; D2S201ESDF-1 receptor; FB22; Fusin; HM89NPYRL; HSY3RR; LAP3; LCR1; LESTRCD184 antigen; Leukocyte-derived seven transmembrane domain receptor; leukocyte-derived seven-transmembrane-domain receptor; lipopolysaccharide-associated protein 3; neuropeptide Y receptor Y3; NPY3RWHIM; NPYR; NPYY3R; seven transmembrane helix receptor; seven-transmembrane-segment receptor, spleen; Stromal cell-derived factor 1 receptor
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

21 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Epithelial-to-mesenchymal transition in FHC-silenced cells: the role of CXCR4/CXCL12 axis
    Authors: I Aversa, F Zolea, C Ieranò, S Bulotta, AM Trotta, MC Faniello, C De Marco, D Malanga, F Biamonte, G Viglietto, G Cuda, S Scala, F Costanzo
    J. Exp. Clin. Cancer Res., 2017;36(1):104.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Extensive Characterization and Comparison of Endothelial Cells Derived from Dermis and Adipose Tissue: Potential Use in Tissue Engineering
    PLoS ONE, 2016;11(11):e0167056.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Mesenchymal stromal cell secretome up-regulates 47 kDa CXCR4 expression, and induce invasiveness in neuroblastoma cell lines.
    Authors: Shankar V, Hori H, Kihira K, Lei Q, Toyoda H, Iwamoto S, Komada Y
    PLoS ONE, 2015;10(3):e0120069.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. IL-24 inhibits lung cancer cell migration and invasion by disrupting the SDF-1/CXCR4 signaling axis.
    Authors: Panneerselvam J, Jin J, Shanker M, Lauderdale J, Bates J, Wang Q, Zhao Y, Archibald S, Hubin T, Ramesh R
    PLoS ONE, 2015;10(3):e0122439.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. PI3K/mTORC2 regulates TGF-beta/Activin signalling by modulating Smad2/3 activity via linker phosphorylation.
    Authors: Yu J, Ramasamy T, Murphy N, Holt M, Czapiewski R, Wei S, Cui W
    Nat Commun, 2015;6(0):7212.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  6. The high-affinity CXCR4 antagonist BKT140 is safe and induces a robust mobilization of human CD34+ cells in patients with multiple myeloma.
    Authors: Peled A, Abraham M, Avivi I, Rowe J, Beider K, Wald H, Tiomkin L, Ribakovsky L, Riback Y, Ramati Y, Aviel S, Galun E, Shaw H, Eizenberg O, Hardan I, Shimoni A, Nagler A
    Clin Cancer Res, 2014;20(2):469-79.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  7. Conversion of peripheral blood NK cells to a decidual NK-like phenotype by a cocktail of defined factors.
    Authors: Cerdeira A, Rajakumar A, Royle C, Lo A, Husain Z, Thadhani R, Sukhatme V, Karumanchi S, Kopcow H
    J Immunol, 2013;190(8):3939-48.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  8. CXCR4 positive and angiogenic monocytes in myocardial infarction.
    Thromb Haemost, 2013;109(2):255-62.
    Species: Human
    Sample Type: Whole Blood
    Application: Flow
  9. KDR identifies a conserved human and murine hepatic progenitor and instructs early liver development.
    Authors: Goldman O, Han S, Sourisseau M, Dziedzic N, Hamou W, Corneo B, D'Souza S, Sato T, Kotton D, Bissig K, Kalir T, Jacobs A, Evans T, Evans M, Gouon-Evans V
    Cell Stem Cell, 2013;12(6):748-60.
  10. A novel chemically directed route for the generation of definitive endoderm from human embryonic stem cells based on inhibition of GSK-3.
    Authors: Bone HK, Nelson AS, Goldring CE, Tosh D, Welham MJ
    J. Cell. Sci., 2011;124(0):1992-2000.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  11. Immunophenotypic characterization of human monocyte subsets: possible implications for cardiovascular disease pathophysiology.
    Authors: Shantsila E, Wrigley B, Tapp L, Apostolakis S, Montoro-Garcia S, Drayson MT, Lip GY
    J. Thromb. Haemost., 2011;9(5):1056-66.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  12. Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver.
    Authors: Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH
    J. Immunol., 2010;184(6):2886-98.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  13. Expression of an exogenous human Oct-4 promoter identifies tumor-initiating cells in osteosarcoma.
    Authors: Levings PP, McGarry SV, Currie TP, Nickerson DM, McClellan S, Ghivizzani SC, Steindler DA, Gibbs CP
    Cancer Res., 2009;69(14):5648-55.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  14. Chemokine-mediated migration of skin-derived stem cells: predominant role for CCL5/RANTES.
    Authors: Kroeze KL, Jurgens WJ, Doulabi BZ, van Milligen FJ, Scheper RJ, Gibbs S
    J. Invest. Dermatol., 2009;129(6):1569-81.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  15. Chemokine production and chemokine receptor expression by human glioma cells: role of CXCL10 in tumour cell proliferation.
    Authors: Maru SV, Holloway KA, Flynn G, Lancashire CL, Loughlin AJ, Male DK, Romero IA
    J. Neuroimmunol., 2008;199(1):35-45.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  16. A Novel Approach for the Derivation of Putative Primordial Germ Cells and Sertoli Cells from Human Embryonic Stem Cells.
    Authors: Bucay N, Yebra M, Cirulli V, Afrikanova I, Kaido T, Hayek A, Montgomery AM
    Stem Cells, 2008;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  17. Chemokine receptors in head and neck cancer: association with metastatic spread and regulation during chemotherapy.
    Authors: Muller A, Sonkoly E, Eulert C, Gerber PA, Kubitza R, Schirlau K, Franken-Kunkel P, Poremba C, Snyderman C, Klotz LO, Ruzicka T, Bier H, Zlotnik A, Whiteside TL, Homey B, Hoffmann TK
    Int. J. Cancer, 2006;118(9):2147-57.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  18. Importance of recruitment of bone marrow-derived CXCR4+ cells in post-infarct cardiac repair mediated by G-CSF.
    Authors: Misao Y, Takemura G, Arai M, Ohno T, Onogi H, Takahashi T, Minatoguchi S, Fujiwara T, Fujiwara H
    Cardiovasc. Res., 2006;71(3):455-65.
    Species: Rabbit
    Sample Type: Whole Cells
    Application: Flow
  19. Chemokine receptor expression in EBV-associated lymphoproliferation in hu/SCID mice: implications for CXCL12/CXCR4 axis in lymphoma generation.
    Authors: Piovan E, Tosello V, Indraccolo S, Cabrelle A, Baesso I, Trentin L, Zamarchi R, Tamamura H, Fujii N, Semenzato G, Chieco-Bianchi L, Amadori A
    Blood, 2005;105(3):931-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  20. CXCL12-CXCR4 interactions modulate prostate cancer cell migration, metalloproteinase expression and invasion.
    Authors: Singh S, Singh UP, Grizzle WE, Lillard JW
    Lab. Invest., 2004;84(12):1666-76.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  21. Chemokine receptor expression on human eosinophils from peripheral blood and bronchoalveolar lavage fluid after segmental antigen challenge.
    Authors: Liu LY, Jarjour NN, Busse WW, Kelly EA
    J. Allergy Clin. Immunol., 2003;112(3):556-62.
    Species: Human
    Sample Type: BALF
    Application: Flow
Expand to show all 21 Citations
Isotype Controls
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Mouse IgG2A PE-conjugated Antibody

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Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

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Average Rating: 5 (Based on 7 reviews)

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We have 7 reviews tested in 1 species: Human.
We have 7 review tested in 1 application: Flow Cytometry.

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Images Ratings Applications Species Reviewed By Date Details
Flow Cytometry Human CXCR4 PE-conjugated Antibody FAB170P
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Excellent
 Flow Human Anonymous 11/27/2017
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Image Details
Flow Cytometry Human CXCR4 PE-conjugated Antibody FAB170P
Flow Cytometry: Human CXCR4 PE-conjugated Antibody [FAB170P]

Summary

ApplicationFlow Cytometry
Sample TestedMDA-MB-231 human breast cancer cell line
SpeciesHuman
Flow Cytometry Human CXCR4 PE-conjugated Antibody FAB170P
Enlarge
Excellent
 Flow Human Anonymous 11/20/2017
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Image Details
Flow Cytometry Human CXCR4 PE-conjugated Antibody FAB170P
Flow Cytometry: Human CXCR4 PE-conjugated Antibody [FAB170P]

Summary

ApplicationFlow Cytometry
Sample TestedMCF7
SpeciesHuman
  Excellent
 Flow Human Anonymous 11/10/2017
Show

Summary

ApplicationFlow Cytometry
Sample TestedJurkat human acute T cell leukemia cell line
SpeciesHuman
Flow Cytometry Human CXCR4 PE-conjugated Antibody FAB170P
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Excellent
 Flow Human Anonymous 11/06/2017
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Image Details
Flow Cytometry Human CXCR4 PE-conjugated Antibody FAB170P
Flow Cytometry: Human CXCR4 PE-conjugated Antibody [FAB170P]

Summary

ApplicationFlow Cytometry
Sample TestedCHO Chinese hamster ovary cell line
SpeciesHuman
Flow Cytometry Human CXCR4 PE-conjugated Antibody FAB170P
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Excellent
 Flow Human Anonymous 08/15/2017
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Image Details
Flow Cytometry Human CXCR4 PE-conjugated Antibody FAB170P
Flow Cytometry: Human CXCR4 PE-conjugated Antibody [FAB170P]

Summary

ApplicationFlow Cytometry
Sample TestedU87 CXCR4 transfected cells
SpeciesHuman
Flow Cytometry Human CXCR4 PE-conjugated Antibody FAB170P
Enlarge
Excellent
 Flow Human Anonymous 08/15/2017
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Image Details
Flow Cytometry Human CXCR4 PE-conjugated Antibody FAB170P
Flow Cytometry: Human CXCR4 PE-conjugated Antibody [FAB170P]

Summary

ApplicationFlow Cytometry
Sample TestedJurkat human acute T cell leukemia cell line
SpeciesHuman

Other Experimental Details

Other Experimental DetailsCell surface receptor level quantification for further animal experiment
Flow Cytometry Human CXCR4 PE-conjugated Antibody FAB170P
Enlarge
Excellent
 Flow Human Anonymous 06/13/2017
Show
Image Details
Flow Cytometry Human CXCR4 PE-conjugated Antibody FAB170P
Flow Cytometry: Human CXCR4 PE-conjugated Antibody [FAB170P]

Summary

ApplicationFlow Cytometry
Sample TestedKG-1 human acute myelogenous leukemia cell line,RS4;11,Molm13,Ramos human Burkitt's lymphoma cell line,Granta-519,HL-60 human acute promyelocytic leukemia cell line
SpeciesHuman

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