Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rabbit

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry, Immunocytochemistry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 12G5
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Product Specifications

Immunogen

CP-MAC-infected SUP-T1 human T cell lymphoblastic lymphoma line
Accession # P61073

Specificity

Detects human CXCR4.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human CXCR4 PE‑conjugated Antibody

Detection of CXCR4 antibody in Human Blood Lymphocytes antibody by Flow Cytometry.

Detection of CXCR4 in Human Blood Lymphocytes by Flow Cytometry.

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CXCR4 PE-conjugated Monoclonal Antibody (Catalog # FAB170P) and Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (FAB4867A) for 15 minutes at 37°C followed by 15 minutes at room temperature. View our protocol for Staining Membrane-associated Proteins.

Detection of CXCR4 in Jurkat vs K562 cells by Flow Cytometry

Jurkat (filled histogram) vs K562 (open histogram) cells were stained with Mouse Anti-Human CXCR4 PE‑conjugated Monoclonal Antibody (Catalog # FAB170P). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CXCR4 PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: see below

Reviewed Applications

Read 12 reviews rated 5 using FAB170P in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCR4

CXCR4, also known as CD184, is a G-protein-linked seven transmembrane spanning receptor that binds stromal cell-derived factor-1 (SDF-1). CXCR4 acts as a co-factor for T-cell tropic HIV-1 and -2 viral entry into cells.

References

  1. Endres, M.J. et al. (1996) Cell 87:745.

Long Name

C-X-C Motif Chemokine Receptor 4

Alternate Names

CD184, D2S201E, FB22, Fusin, HM89, HSY3RR, LAP-3, LAP3, LCR1, LESTR, NPY3R, NPYRL, NPYY3R, WHIMS

Entrez Gene IDs

7852 (Human); 12767 (Mouse); 60628 (Rat); 483900 (Canine); 493676 (Feline)

Gene Symbol

CXCR4

UniProt

Additional CXCR4 Products

Product Documents for Human CXCR4 PE‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CXCR4 PE‑conjugated Antibody

For research use only

Citations for Human CXCR4 PE‑conjugated Antibody

Customer Reviews for Human CXCR4 PE‑conjugated Antibody (12)

5 out of 5
12 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Showing  1 - 5 of 12 reviews Showing All
Filter By:
  • Human CXCR4 PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Jurkat human acute T cell leukemia cell line
    Species: Human
    Verified Customer | Posted 03/30/2018
  • Human CXCR4 PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Jurkat human acute T cell leukemia cell line
    Species: Human
    Verified Customer | Posted 03/15/2018
  • Human CXCR4 PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: MDA-MB-231 human breast cancer cell line
    Species: Human
    Verified Customer | Posted 03/12/2018
  • Human CXCR4 PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: MDA-MB-231 human breast cancer cell line
    Species: Human
    Verified Customer | Posted 11/27/2017
    Human CXCR4 PE‑conjugated Antibody FAB170P
  • Human CXCR4 PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: MCF7
    Species: Human
    Verified Customer | Posted 11/20/2017
    Human CXCR4 PE‑conjugated Antibody FAB170P
  • Human CXCR4 PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Jurkat human acute T cell leukemia cell line
    Species: Human
    Verified Customer | Posted 11/10/2017
  • Human CXCR4 PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: CHO Chinese hamster ovary cell line
    Species: Human
    Verified Customer | Posted 11/06/2017
    Human CXCR4 PE‑conjugated Antibody FAB170P
  • Human CXCR4 PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: U87 CXCR4 transfected cells
    Species: Human
    Verified Customer | Posted 08/15/2017
    Human CXCR4 PE‑conjugated Antibody FAB170P
  • Human CXCR4 PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Jurkat human acute T cell leukemia cell line
    Species: Human
    Verified Customer | Posted 08/15/2017
    Cell surface receptor level quantification for further animal experiment
    Human CXCR4 PE‑conjugated Antibody FAB170P
  • Human CXCR4 PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: KG-1 human acute myelogenous leukemia cell line, RS4;11, Molm13, Ramos human Burkitt's lymphoma cell line, Granta-519 and HL-60 human acute promyelocytic leukemia cell line
    Species: Human
    Verified Customer | Posted 06/13/2017
    Human CXCR4 PE‑conjugated Antibody FAB170P
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID:23456977
    Species: Human
    Verified Customer | Posted 01/21/2015
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID: 23456977
    Species: Human
    Verified Customer | Posted 01/21/2015

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