Human CXCR4 Antibody (12G5) [Biotin]

Name: Human CXCR4 Antibody (12G5) [Biotin]
Brand: R&D Systems
SKU: FAB170B
Price: 289 USD
Availability: InStock

R&D Systems | Catalog # FAB170B

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Citations (7)

Product Documents

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Neutralization, Flow Cytometry, Immunocytochemistry

Label

Biotin

Antibody Source

Monoclonal Mouse IgG_2A Clone # 12G5

View all CXCR4 Primary Antibodies »

Product Specifications

Immunogen

CP-MAC-infected SUP-T1 human T cell lymphoblastic lymphoma line
Accession # P61073

Specificity

Detects human CXCR4.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Scientific Data Images for Human CXCR4 Antibody (12G5) [Biotin]

Detection of CXCR4 in Human Blood Lymphocytes by Flow Cytometry.

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CXCR4 Biotinylated Monoclonal Antibody (Catalog # FAB170B) followed by Streptavidin-Phycoerythrin (Catalog # F0040) and Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A). View our protocol for Staining Membrane-associated Proteins.

Detection of CXCR4 in Jurkat vs K562 cells by Flow Cytometry

Jurkat (filled histogram) vs K562 (open histogram) cells were stained with Mouse Anti-Human CXCR4 Biotinylated Monoclonal Antibody (Catalog # FAB170B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CXCR4 Antibody (12G5) [Biotin]

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: see below

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCR4

CXCR4, also known as CD184, is a G-protein-linked seven transmembrane spanning receptor that binds stromal cell-derived factor-1 (SDF-1). CXCR4 acts as a co-factor for T-cell tropic HIV-1 and -2 viral entry into cells.