Human Cytochrome c Quantikine ELISA Kit

(22 citations)   
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Lysates (20 uL), Subcellular Fractions (100 uL)
  • Sensitivity
    0.31 ng/mL
  • Assay Range
    0.6 - 20 ng/mL (Cell Lysates, Subcellular Fractions)
  • Specificity
    Natural human Cytochrome c
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    Interference observed with other substances.
Control Available
QC75, Quantikine Immunoassay Control Set 795 Human Cytochrome c - Please Inquire
Product Summary
The Quantikine Human Cytochrome c Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human cytochrome c in cell lysates.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Lysates, Subcellular Fractions
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean3.647.3110.73.546.7410.2
Standard Deviation0.150.30.420.180.340.51
CV%4.14.13.95.155

Linearity
To assess the linearity of the assay, six cell lysates containing high concentrations of Cytochrome c were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Cytochrome c Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Cytochrome c
Cytochrome c is a small, highly conserved, heme-containing protein involved in mitochondrial electron transport. Cytochrome c is released from the mitochondria in response to pro-apoptotic stimuli and serves as a cofactor in initiating the activation of the APAF-1/Caspase-9 complex. The effect of specific proteins and/or small molecules on the initiation of the intrinsic apoptotic pathway can be evaluated in an in vitro system of isolated mitochondria by monitoring the release of cytochrome c.
    • Entrez Gene IDs
      54205 (Human);
    • Alternate Names
      CYCHCS; CYCS; Cytochrome c; cytochrome c, somatic; THC4;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Calibrator Diluent
    4.   Add 100 µL of Calibrator Diluent to each well.

    5. 100 µL Standard, Control, or Sample
    6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    22 Citations: Showing 1 - 10
    Filter your results:

    Species
    Sample Type
    1. Critical Role of the Neonatal Fc Receptor (FcRn) in the Pathogenic Action of Antimitochondrial Autoantibodies Synergizing with Anti-desmoglein Autoantibodies in Pemphigus Vulgaris.
      Authors: Chen Y, Chernyavsky A, Webber R, Grando S, Wang P
      J Biol Chem, 2015;290(39):23826-37.
      Species: Human
      Sample Type: Cell Culture Supernates
    2. Oxidative stress-mediated apoptosis induced by ethanolic mango seed extract in cultured estrogen receptor positive breast cancer MCF-7 cells.
      Authors: Abdullah A, Mohammed A, Rasedee A, Mirghani M
      Int J Mol Sci, 2015;16(2):3528-36.
      Species: Human
      Sample Type: Cell Lysates
    3. Cytokine and nitric oxide levels in patients with sepsis--temporal evolvement and relation to platelet mitochondrial respiratory function.
      Authors: Sjovall F, Morota S, Asander Frostner E, Hansson M, Elmer E
      PLoS ONE, 2014;9(5):e97673.
      Species: Human
      Sample Type: Cell Lysates
    4. Antagonizing effects and mechanisms of afzelin against UVB-induced cell damage.
      Authors: Shin S, Jung E, Kim S, Kim J, Kim E, Lee J, Park D
      PLoS ONE, 2013;8(4):e61971.
      Species: Human
      Sample Type: Cell Culture Supernates
    5. Antimitochondrial autoantibodies in pemphigus vulgaris: a missing link in disease pathophysiology.
      Authors: Marchenko S, Chernyavsky AI, Arredondo J, Gindi V, Grando SA
      J. Biol. Chem., 2010;285(6):3695-704.
      Species: Human
      Sample Type: Cell Lysates
    6. Roux-en-Y gastric bypass reverses renal glomerular but not tubular abnormalities in excessively obese diabetics.
      Authors: Saliba J, Kasim NR, Tamboli RA, Isbell JM, Marks P, Feurer ID, Ikizler A, Abumrad NN
      Surgery, 2010;147(2):282-7.
      Species: Human
      Sample Type: Urine
    7. Aurora kinase A is a target of Wnt/beta-catenin involved in multiple myeloma disease progression.
      Authors: Dutta-Simmons J, Zhang Y, Gorgun G, Gatt M, Mani M, Hideshima T, Takada K, Carlson NE, Carrasco DE, Tai YT, Raje N, Letai AG, Anderson KC, Carrasco DR
      Blood, 2009;114(13):2699-708.
      Species: Human
      Sample Type: Cell Culture Supernates
    8. Mitochondrial-targeted active Akt protects SH-SY5Y neuroblastoma cells from staurosporine-induced apoptotic cell death.
      Authors: Mookherjee P, Quintanilla R, Roh MS, Zmijewska AA, Jope RS, Johnson GV
      J. Cell. Biochem., 2007;102(1):196-210.
      Species: Human
      Sample Type: Cell Culture Supernates
    9. Proton pump inhibitors induce apoptosis of human B-cell tumors through a caspase-independent mechanism involving reactive oxygen species.
      Authors: De Milito A, Iessi E, Logozzi M, Lozupone F, Spada M, Marino ML, Federici C, Perdicchio M, Matarrese P, Lugini L, Nilsson A, Fais S
      Cancer Res., 2007;67(11):5408-17.
      Species: Human
      Sample Type: Cell Lysates
    10. Glycyrrhizin protection against 3-morpholinosydnonime-induced mitochondrial dysfunction and cell death in lung epithelial cells.
      Authors: Lee CS, Kim YJ, Han ES
      Life Sci., 2007;80(19):1759-67.
      Species: Human
      Sample Type: Cell Lysates
    11. Apolipoprotein-J prevention of fetal cardiac myoblast apoptosis induced by ethanol.
      Authors: Li Y, Sagar MB, Wassler M, Shelat H, Geng YJ
      Biochem. Biophys. Res. Commun., 2007;357(1):157-61.
      Species: Canine
      Sample Type: Cell Lysates
    12. The apoptosis effect of hispolon from Phellinus linteus (Berkeley & Curtis) Teng on human epidermoid KB cells.
      Authors: Chen W, He FY, Li YQ
      J Ethnopharmacol, 2006;105(1):280-5.
      Species: Human
      Sample Type: Cell Lysates
    13. Hierarchical regulation of mitochondrion-dependent apoptosis by BCL-2 subfamilies.
      Authors: Kim H, Rafiuddin-Shah M, Tu HC, Jeffers JR, Zambetti GP, Hsieh JJ, Cheng EH
      Nat. Cell Biol., 2006;8(12):1348-58.
      Species: Human
      Sample Type: Cell Culture Supernates
    14. Beauvericin induces cytotoxic effects in human acute lymphoblastic leukemia cells through cytochrome c release, caspase 3 activation: the causative role of calcium.
      Authors: Jow GM, Chou CJ, Chen BF, Tsai JH
      Cancer Lett., 2004;216(2):165-73.
      Species: Human
      Sample Type: Cell Lysates
    15. Differential response of MG132 cytotoxicity against small cell lung cancer cells to changes in cellular GSH contents.
      Authors: Bang JH, Han ES, Lim I
      Biochem. Pharmacol., 2004;68(4):659-66.
      Species: Human
      Sample Type: Cell Lysates
    16. Ebselen inhibits p38 mitogen-activated protein kinase-mediated endothelial cell death by hydrogen peroxide.
      Authors: Ali N, Yoshizumi M, Tsuchiya K, Kyaw M, Fujita Y, Izawa Y, Abe S, Kanematsu Y, Kagami S, Tamaki T
      Eur. J. Pharmacol., 2004;485(1):127-35.
      Species: Human
      Sample Type: Cell Lysates
    17. The mitochondria-regulated death pathway mediates asbestos-induced alveolar epithelial cell apoptosis.
      Authors: Panduri V, Weitzman SA, Chandel N, Kamp DW
      Am. J. Respir. Cell Mol. Biol., 2003;28(2):241-8.
      Species: Human
      Sample Type: Cell Lysates
    18. Release of intact, monomeric cytochrome c from apoptotic and necrotic cells.
      Authors: Jemmerson R, LaPlante B, Treeful A
      Cell Death Differ., 2002;9(5):538-48.
      Species: Human
      Sample Type: Cell Culture Supernates
    19. Bcl-2 phosphorylation and proteasome-dependent degradation induced by paclitaxel treatment: consequences on sensitivity of isolated mitochondria to Bid.
      Authors: Brichese L, Barboule N, Heliez C, Valette A
      Exp. Cell Res., 2002;278(1):101-11.
      Species: Human
      Sample Type: Cell Lysates
    20. Caspases as key executors of methyl selenium-induced apoptosis (anoikis) of DU-145 prostate cancer cells.
      Authors: Jiang C, Wang Z, Ganther H, Lu J
      Cancer Res., 2001;61(7):3062-70.
      Species: Human
      Sample Type: Cell Lysates
    21. Fas-mediated apoptosis in neuroblastoma requires mitochondrial activation and is inhibited by FLICE inhibitor protein and Bcl-2.
      Authors: Poulaki V, Mitsiades N, Romero ME, Tsokos M
      Cancer Res., 2001;61(12):4864-72.
      Species: Human
      Sample Type: Cell Lysates
    22. Epithelial cells infected with Chlamydophila pneumoniae (Chlamydia pneumoniae) are resistant to apoptosis.
      Authors: Rajalingam K, Al-Younes H, Muller A, Meyer TF, Szczepek AJ, Rudel T
      Infect. Immun., 2001;69(12):7880-8.
      Species: Human
      Sample Type: Cell Lysates
    Expand to show all 22 Citations
    ELISA Controls
    Description Application Cat# Citations Images  

    Quantikine Immunoassay Control Set 795 Human Cytochrome c

    Ctrl QC75
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    Supplemental ELISA Products
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    Quantikine ELISA Wash Buffer 1

    ELISA WA126 6
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    Cell Lysis Buffer 2

    ELISA 895347 2
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