Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Desmocollin-3 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Desmocollin-3
Arg136-Lys690
Accession # Q14574

Specificity

Detects human Desmocollin-3 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Desmocollin-2 is observed and less than 1% cross-reactivity with rhDesmocollin-1 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human Desmocollin‑3 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Desmocollin‑3 (Catalog # 4630-DC)

Reviewed Applications

Read 1 review rated 5 using AF4630 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Desmocollin-3

Desmocollin-3 is a 100‑110 kDa type I transmembrane glycoprotein that belongs to the cadherin family of calcium dependent adhesion molecules (1‑3). The human Desmocollin-3 is synthesized with a 108 amino acid (aa) propeptide. The mature protein consists of a 555 aa extracellular domain (ECD) that contains five cadherin‑like domains, a 21 aa transmembrane segment, and a 185 aa cytoplasmic domain (4, 5). Within the ECD, human Desmocollin-3 shares 78% aa sequence identity with mouse and rat Desmocollin-3. It shares 52% and 64% aa sequence identity with Desmocollin-1 and -2, respectively. A splice variant of human Desmocollin-3 has a truncated and substituted cytoplasmic domain (4, 6). Desmocollin-3 is one of the principal components of desmosomes which form adhesive contacts between epithelial cells (1, 2). It is expressed in the basal and suprabasal layers of stratified epithelia in many tissues (5, 7, 8). Desmocollin-1 and -2, by contrast, are preferentially localized in the more superficial layers (7). In mouse, Desmocollin-3 is expressed throughout embryogenesis and is required for non‑desmosomal functions during early development (9, 10). During apoptosis, both isoforms of Desmocollin-3 are proteolytically cleaved by caspases and metalloproteases (11). Desmocollin-3 can be up‑regulated or down‑regulated in carcinoma (6, 12, 13).

References

  1. Kottke, M.D. et al. (2006) J. Cell Sci. 119:797.
  2. Garrod, D.R. et al. (2002) Mol. Memb. Biol. 19:81.
  3. Leckband, D. and A. Prakasam (2006) Annu. Rev. Biomed. Eng. 8:259.
  4. Kawamura, K. et al. (1994) J. Biol. Chem. 269:26295.
  5. King, I.A. et al. (1995) J. Invest. Dermatol. 105:314.
  6. Klus, G.T. et al. (2001) Int. J. Oncol. 19:169.
  7. North, A.J. et al. (1996) Proc. Natl. Acad. Sci. 93:7701.
  8. Nuber, U.A. et al. (1996) Eur. J. Cell Biol. 71:1.
  9. Chidgey, M.A.J. et al. (1997) Dev. Dyn. 210:315.
  10. Den, Z. et al. (2006) J. Cell Sci. 119:482.
  11. Weiske, J. et al. (2001) J. Biol. Chem. 276:41175.
  12. Wang, L. et al. (2007) Virchows Arch. Sept. 11 epub.
  13. Boelens, M.C. et al. (2007) J. Clin. Pathol. 60:608.

Alternate Names

CDHF3, Desmocollin3, DSC3, DSC4, HT-CP

Entrez Gene IDs

1825 (Human); 13507 (Mouse); 307563 (Rat)

Gene Symbol

DSC3

UniProt

Q14574

Additional Desmocollin-3 Products

Product Documents for Human Desmocollin‑3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Desmocollin‑3 Antibody

For research use only

Related Research Areas

Cadherin Superfamily

Customer Reviews for Human Desmocollin‑3 Antibody (1)

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  • Human Desmocollin-3 Antibody
    Name: Preethi Vijayaraj
    Application: Immunohistochemistry
    Sample Tested: Adult lung
    Species: Human
    Verified Customer | Posted 02/15/2019
    Human Desmocollin‑3 Antibody AF4630

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