Human DLL1 APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects recombinant human DLL1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse DLL4 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 251127
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human DLL1
    Accession # AAG09716
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of DLL1 in T98G Human Glioblastoma Cell Line by Flow Cytometry. T98G human glioblastoma cell line was stained with Mouse Anti-Human DLL1 APC‑conjugated Monoclonal Antibody (Catalog # FAB1818A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: DLL1

Delta-like protein 1 (DLL1) is a 90-100 kDa type I transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. Mature human DLL1 consists of a 528 amino acid (aa) extracellular domain (ECD) with one DSL domain and eight EGF-like repeats, a 23 aa transmembrane segment, and a 155 aa cytoplasmic domain (1). Within the ECD, human DLL1 shares 91% aa sequence identity with mouse and rat DLL1. It shares 26%, 37%, and 54% aa sequence identity with DLL2, 3, and 4, respectively. A 60 kDa ECD fragment released by ADAM9, 12, or 17 mediated proteolysis, promotes the proliferation of hematopoietic progenitor cells (2, 3). The residual membrane-bound portion of DLL1 can be cleaved by presenilin-dependent gamma -secretase, enabling the cytoplasmic domain to migrate to the nucleus (4). DLL1 localizes to adherens junctions on neuronal processes through its association with the scaffolding protein MAGI1 (5). DLL1 is widely expressed, and it plays an important role in embryonic somite formation, cochlear hair cell differentiation, plus B and T lymphocyte differentiation (6-11). The upregulation of DLL1 in arterial endothelial cells following injury or angiogenic stimulation is central to postnatal arteriogenesis (12). DLL1 is also overexpressed in cervical carcinoma and glioma and contributes to tumor progression (1, 13).

  • References:
    1. Gray, G.E. et al. (1999) Am. J. Pathol. 154:785. 
    2. Dyczynska, E. et al. (2007) J. Biol. Chem. 282:436. 
    3. Karanu, F.N. et al. (2001) Blood 97:1960. 
    4. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751. 
    5. Mizuhara, E. et al. (2005) J. Biol. Chem. 280:26499. 
    6. Takahashi, Y. et al. (2003) Development 130:4259. 
    7. Teppner, I. et al. (2007) BMC Dev. Biol. 7:68.
    8. Kiernan, A.E. et al. (2005) Development 132:4353.
    9. Schmitt, T.M. and J.C. Zuniga-Pflucker (2002) Immunity 17:749.
    10. Hozumi, K. et al. (2004) Nat. Immunol. 5:638.
    11. Santos, M.A. et al. (2007) Proc. Natl. Acad. Sci. 104:15454.
    12. Limbourg, A. et al. (2007) Circ. Res. 100:363.
    13. Purow, B.W. et al. (2005) Cancer Res. 65:2353.
  • Long Name:
    Delta-like 1
  • Entrez Gene IDs:
    28514 (Human); 13388 (Mouse); 84010 (Rat)
  • Alternate Names:
    delta (Drosophila)-like 1; Delta 1; Delta; Delta1; delta-like 1 (Drosophila); delta-like protein 1; DL1; Drosophila Delta homolog 1; H-Delta-1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B APC-conjugated Antibody

Ctrl IC0041A 4  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human DLL1 APC-conjugated Antibody?

Submit a review and receive a $25 amazon gift card if you include an image - $10 Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Certificate of Analysis Lookup

The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided

Certificate of Analysis Lookup

Research Areas of Interest
R&D Systems Guarantee

Associated Pathways


Customer Information