Human DPPIV/CD26 Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1180G-100UG
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Human DPPIV/CD26 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human DPPIV/CD26 in ELISAs and Western blots. In ELISAs and Western blots, no cross-reactivity with recombinant human Cathepsin A or recombinant mouse DPPIV is observed.
Source
Monoclonal Rat IgG2a Clone # 222113
Immunogen
Mouse myeloma cell line NS0-derived recombinant human DPPIV/CD26
Asp34-Pro766
Accession # Q53TN1
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
Human whole blood lymphocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: DPPIV/CD26

DPPIV/CD26 (EC 3.4.14.5) is a serine exopeptidase that releases Xaa-Pro dipeptides from the N-terminus of oligo- and polypeptides (1, 2). It is a type II membrane protein consisting of a short cytoplasmic tail, a transmembrane domain, and a long extracellular domain (3‑5). The extracellular domain contains glycosylation sites, a cysteine-rich region and the catalytic active site (Ser, Asp and His charge relay system). The amino acid sequence of the mouse DPPIV/CD26 extracellular domain is 84% and 91% identical to the human and rat counterparts, respectively. In the native state, DPPIV/CD26 is present as a noncovalently linked homodimer on the cell surface of a variety of cell types. The soluble form is also detectable in human serum and other body fluids, the levels of which may have clinical significance in patients with cancer, liver and kidney diseases, and depression. DPPIV/CD26 plays an important role in many biological and pathological processes. It functions as T cell-activating molecule (THAM). It serves as a cofactor for entry of HIV in CD4+ cells (6). It binds adenosine deaminase, the deficiency of which causes severe combined immunodeficiency disease in humans (7). It cleaves chemokines such as stromal-cell-derived factor 1 alpha and macrophage-derived chemokine (8, 9). It degrades peptide hormones such as glucagon (10). It truncates procalcitonin, a marker for systemic bacterial infections with elevated levels detected in patients with thermal injury, sepsis and severe infection, and in children with bacterial meningitis (11).

References
  1. Misumi and Ikehara (1998) in Handbook of Proteolytic Enzymes. Barrett, et al. (eds) San Diego: Academic Press, p. 378.
  2. Ikehara, et al. (1994) Methods Enzymol. 244:215.
  3. Marguet, et al. (1992) J. Biol. Chem. 267:2200.
  4. Bernard, et al. (1994) Biochemistry 33:15204.
  5. Viver, et al. (1991) J. Immunol. 147:447.
  6. Callebaut, et al. (1993) Science 262:2045.
  7. Kameoka, et al. (1993) Science 261:466.
  8. Ohtsuki, et al. (1998) FEBS Lett. 431:236.
  9. Proost, et al. (1999) J. Biol. Chem. 274:3988.
  10. Hinke, et al. (2000) J. Biol. Chem. 275:3827.
  11. Wrenger, et al. (2000) FEBS Lett. 466:155.
Long Name
Dipeptidyl-peptidase IV
Entrez Gene IDs
1803 (Human); 13482 (Mouse); 102133935 (Cynomolgus Monkey)
Alternate Names
ADABP; ADCP-2; ADCP2DPP IV; Adenosine deaminase complexing protein 2TP103; CD26 antigen; CD26; CD26T-cell activation antigen CD26; dipeptidyl peptidase 4; Dipeptidyl peptidase IV; dipeptidylpeptidase 4; dipeptidyl-peptidase 4; dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2); DPP4; DPPIV; EC 3.4.14.5

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