Human DPPIV/CD26 Antibody Summary
Accession # Q53TN1
Human DPPIV/CD26 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human DPPIV/CD26 by Western Blot. Western blot shows lysate of LoVo human colorectal adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human DPPIV/CD26 Monoclonal Antibody (Catalog # MAB1180) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for DPPIV/CD26 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of DPPIV/CD26 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Rat Anti-Human DPPIV/CD26 Monoclonal Antibody (Catalog # MAB1180, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram) followed by Anti-mouse PE-conjugated secondary antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
DPPIV/CD26 (EC 188.8.131.52) is a serine exopeptidase that releases Xaa-Pro dipeptides from the N-terminus of oligo- and polypeptides (1, 2). It is a type II membrane protein consisting of a short cytoplasmic tail, a transmembrane domain, and a long extracellular domain (3‑5). The extracellular domain contains glycosylation sites, a cysteine-rich region and the catalytic active site (Ser, Asp and His charge relay system). The amino acid sequence of the mouse DPPIV/CD26 extracellular domain is 84% and 91% identical to the human and rat counterparts, respectively. In the native state, DPPIV/CD26 is present as a noncovalently linked homodimer on the cell surface of a variety of cell types. The soluble form is also detectable in human serum and other body fluids, the levels of which may have clinical significance in patients with cancer, liver and kidney diseases, and depression. DPPIV/CD26 plays an important role in many biological and pathological processes. It functions as T cell-activating molecule (THAM). It serves as a cofactor for entry of HIV in CD4+ cells (6). It binds adenosine deaminase, the deficiency of which causes severe combined immunodeficiency disease in humans (7). It cleaves chemokines such as stromal-cell-derived factor 1 alpha and macrophage-derived chemokine (8, 9). It degrades peptide hormones such as glucagon (10). It truncates procalcitonin, a marker for systemic bacterial infections with elevated levels detected in patients with thermal injury, sepsis and severe infection, and in children with bacterial meningitis (11).
- Misumi and Ikehara (1998) in Handbook of Proteolytic Enzymes. Barrett, et al. (eds) San Diego: Academic Press, p. 378.
- Ikehara, et al. (1994) Methods Enzymol. 244:215.
- Marguet, et al. (1992) J. Biol. Chem. 267:2200.
- Bernard, et al. (1994) Biochemistry 33:15204.
- Viver, et al. (1991) J. Immunol. 147:447.
- Callebaut, et al. (1993) Science 262:2045.
- Kameoka, et al. (1993) Science 261:466.
- Ohtsuki, et al. (1998) FEBS Lett. 431:236.
- Proost, et al. (1999) J. Biol. Chem. 274:3988.
- Hinke, et al. (2000) J. Biol. Chem. 275:3827.
- Wrenger, et al. (2000) FEBS Lett. 466:155.
Citations for Human DPPIV/CD26 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
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Experimental infection of dromedaries with Middle East respiratory syndrome-Coronavirus is accompanied by massive ciliary loss and depletion of the cell surface receptor dipeptidyl peptidase 4
Authors: AK Haverkamp, A Lehmbecker, I Spitzbarth, W Widagdo, BL Haagmans, J Segalés, J Vergara-Al, A Bensaid, JMA van den Br, ADME Osterhaus, W Baumgärtne
Sci Rep, 2018;8(1):9778.
Sample Types: Whole Cells
Applications: Flow Cytometry
Engineering a stable CHO cell line for the expression of a MERS-coronavirus vaccine antigen
Authors: MP Nyon, L Du, CK Tseng, CA Seid, J Pollet, KS Naceanceno, A Agrawal, A Algaissi, BH Peng, W Tai, S Jiang, ME Bottazzi, U Strych, PJ Hotez
Sample Types: Cell Lysates
Applications: Western Blot
Involvement of DPP-IV catalytic residues in enzyme-saxagliptin complex formation.
Authors: Metzler WJ, Yanchunas J, Weigelt C
Protein Sci., 2008;17(2):240-50.
Sample Types: Recombinant Protein
Applications: ELISA Development
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