Human DR3/TNFRSF25 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF943
Product Details
Citations (3)
FAQs
Supplemental Products
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Human DR3/TNFRSF25 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human DR3/TNFRSF25 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse DR3 is observed, and less than 1% cross-reactivity with recombinant human (rh) Fas and rhTNF RI is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human DR3/TNFRSF25
Gln25-Phe201
Accession # Q93038
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human DR3/TNFRSF25 Fc Chimera (Catalog # 943-D3)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DR3/TNFRSF25

Death receptor 3 (DR3), also known as lymphocyte-associated receptor of death (LARD), WSL-1, APO3, TRAMP and TR3, is a glycoprotein belonging to the TNF receptor superfamily (TNFRSF) (1‑5). DR3 was formerly designated TNFRSF12 when it was thought to be a receptor for TWEAK/TNFSF12 (6). However, work disavowed the DR3:TWEAK interaction and DR3 is now designated TNFRSF 25 (7). By alternative splicing, at least 11 distinct human DR3 transcripts encoding secreted or type I membrane proteins exist (7). The human DR3 isoform 1 cDNA encodes a 417 amino acid residue (aa) transmembrane precursor with a 24 aa signal peptide, a 175 aa extracellular domain containing four cysteine-rich repeats and two potential N-glycosylation sites, a 21 aa transmembrane region and a 195 aa cytoplasmic region with one death domain. DR3 is one of six within the TNF R superfamily that contains a death domain in its cytooplasmic region. It is most closely related to TNF R1 and FAS/CD95, sharing 29% and 23% aa sequence identity, respectively. DR3 is expressed primarily in tissues enriched in lymphocytes. Whereas naïve B and T cells express multiple truncated DR3 isoforms but not the transmembrane isoform 1, upon T cell activation, expression of the transmembrane DR3 isoform 1 predominates. TL1A/VEGI, a TNF superfamily ligand, has been shown to bind and activate DR3 (8). Depending on the cell context, ligation of DR3 by TL1A can trigger one of two signaling pathways. On primary T cells, TL1A induces NF-kappa -B activation and a costimulatory signal to increase IL-2 responsiveness and the secretion of proinflammatory cytokines. However, in a tumor cell line, TF-1, TL1A has been shown to induce caspase activity and apoptosis. In DR3-null mice, an impairment of negative selection and anti-CD3-mediated thymocyte apoptosis is observed.

References
  1. Chinnaiyan, A.M. et al. (1996) Science 274:990.
  2. Kitson, J. et al. (1996) Nature 384:372.
  3. Bodmer, J-L. et al. (1997) Immunity 6:79.
  4. Screaton, G.R. et al. (1997) Proc. Nat. Acad. Sci. USA 94:4615.
  5. Marsters, S.A. et al. (1996) Curr. Biol. 6:1669.
  6. Marsters, S.A. et al. (1998) Curr. Biol. 8:525.
  7. Kaptein, A. et al. (2000) FEBS Lett. 485:135.
  8. Migone, T-S. et al. (2002) Immunity 16:479.
Long Name
Death Receptor 3
Entrez Gene IDs
8718 (Human); 85030 (Mouse)
Alternate Names
APO3; Apo-3; Apoptosis-inducing receptor AIR; Apoptosis-mediating receptor DR3; Apoptosis-mediating receptor TRAMP; Death receptor 3; DR3; DR3member 12 (translocatingchain-association membrane protein); LARD; Lymphocyte-associated receptor of death; Protein WSL; Protein WSL-1; TNFRSF25; TRAMP; tumor necrosis factor receptor superfamily, member 25; WSL; WSL1; WSL-1

Product Datasheets

Citations for Human DR3/TNFRSF25 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Directed evolution of a soluble human DR3 receptor for the inhibition of TL1A induced cytokine secretion
    Authors: I Levin, M Zaretsky, A Aharoni
    PLoS ONE, 2017;12(3):e0173460.
    Species: Human
    Sample Types: Whole Cells
    Applications: ELISA Development
  2. Death Receptor 3 signaling enhances proliferation of human regulatory T cells
    Authors: S Bittner, G Knoll, M Ehrenschwe
    FEBS Lett, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Human group3 innate lymphoid cells express DR3 and respond to TL1A with enhanced IL-22 production and IL-2-dependent proliferation.
    Authors: Ahn Y, Weeres M, Neulen M, Choi J, Kang S, Heo D, Bergerson R, Blazar B, Miller J, Verneris M
    Eur J Immunol, 2015;45(8):2335-42.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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