Human E-Selectin/CD62E Antibody

(6 citations)   
  • Species Reactivity
    Human
  • Specificity
    This antibody has been screened using CHO cells transfected with cDNAs for E-Selectin, P-Selectin, L-Selectin, ICAM-1 and VCAM‑1. This antibody has shown to be only reactive with E-Selectin.
  • Source
    Polyclonal Goat Serum
  • Purification
    N/A
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human E-Selectin/CD62E
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in Serum.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1:1000 dilution
    Recombinant Human E-Selectin/CD62E Fc Chimera (Catalog # 724-ES)
  • Immunohistochemistry
    1:100 dilution
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
E‑Selectin/CD62E in Human Kidney Cancer Tissue. E‑Selectin/CD62E was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human E‑Selectin/
CD62E Polyclonal Antibody (Catalog # BBA18) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained (brown) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute in 0.5 mL of sterile water.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: E-Selectin/CD62E

E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107 - 115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha, and demonstrates peak expression at 4 hours, and decay at 24 hours, in response to activation. E-Selectin ligands, expressed on neutrophils, monocytes, and a subset of memory T cells, are sialylated, fucosylated molecules which bind to the lectin domain of E-Selectin. Immunocytochemical techniques have demonstrated the expression of E-Selectin on healthy and diseased tissue. The human and mouse
E‑Selectin proteins share 81% amino acid similarity.

E-Selectin mediates the attachment of flowing leukocytes to the blood vessel wall during inflammation by binding to E-Selectin ligands on leukocytes. These interactions are labile and permit leukocytes to roll along the vascular endothelium in the direction of blood flow. This initial interaction is followed by a stronger interaction involving ICAM-1 and VCAM-1 that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue.

ELISA techniques have shown that detectable levels of soluble E-Selectin are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of E-Selectin may be elevated in subjects with a variety of pathological conditions.

  • Entrez Gene IDs:
    6401 (Human); 20339 (Mouse); 25544 (Rat)
  • Alternate Names:
    CD62 antigen-like family member E; CD62E antigen; CD62E; ELAM; ELAM1; ELAM-1; ELAM1E-selectin; endothelial adhesion molecule 1; Endothelial leukocyte adhesion molecule 1; ESEL; E-Selectin; LECAM2; leukocyte endothelial cell adhesion molecule 2; Leukocyte-endothelial cell adhesion molecule 2; SELE; selectin E
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
Filter your results:

Species
Applications
Sample Type
  1. Outer membrane vesicles derived from Escherichia coli up-regulate expression of endothelial cell adhesion molecules in vitro and in vivo.
    Authors: Kim J, Yoon Y, Lee J, Choi E, Yi N, Park K, Park J, Lotvall J, Kim Y, Gho Y
    PLoS ONE, 2013;8(3):e59276.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. ERK5 protein promotes, whereas MEK1 protein differentially regulates, the Toll-like receptor 2 protein-dependent activation of human endothelial cells and monocytes.
    Authors: Wilhelmsen K, Mesa K, Lucero J, Xu F, Hellman J
    J Biol Chem, 2012;287(32):26478-94.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. Endothelial adhesion molecule expression is unaltered in the peripheral nerve from patients with AIDS and distal sensory polyneuropathy.
    Authors: Fenzi F, Rossi F, Rava M, Cavallaro T, Ferrari S, Rizzuto N
    J. Neuroimmunol., 2006;178(1):111-6.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  4. Trafficking pathways and characterization of CD4 and CD8 cells recruited to the skin of humans experimentally infected with Haemophilus ducreyi.
    Authors: Humphreys TL, Baldridge LA, Billings SD, Campbell JJ, Spinola SM
    Infect. Immun., 2005;73(7):3896-902.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  5. TNFalpha increases the inflammatory response to vascular balloon injury without accelerating neointimal formation.
    Authors: Miller AM, McPhaden AR, Preston A, Wadsworth RM, Wainwright CL
    Atherosclerosis, 2005;179(1):51-9.
    Species: Rabbit
    Sample Type: Whole Tissue
    Application: Neut
  6. E-selectin and ICAM-1 are incorporated into detergent-insoluble membrane domains following clustering in endothelial cells.
    Authors: Tilghman RW, Hoover RL
    FEBS Lett., 2002;525(1):83-7.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
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