Human E2F-1 Alexa Fluor® 647-conjugated Antibody

Catalog #: AF4825R Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4825R-100UG

Save 15% on Select RUO Reagents. See Details.

All E2F-1 Antibodies

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human E2F-1 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human E2F-1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human E2F-2, -3, -4, -5, -6, -7, and -8 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human E2F-1
Val264-Phe437
Accession # Q01094

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: E2F-1

E2F-1 (adenovirus E2 gene promoter region binding factor 1; also retinoblastoma [RB]-binding protein 3/RBP3) is a 57 - 60 kDa nuclear member of the E2F/DP family of transcription factors. It has a dual function, mediating both cell proliferation and apoptosis in a context-dependent manner. Human E2F-1 is 437 amino acids (aa) in length. It contains a cyclin A: CDK2 binding site (aa 67 - 108), a DNA-binding region (aa 110 - 194), and an RB protein binding motif (aa 409 - 426) that, when engaged, inactivates E2F-1. There is one potential in-frame 23 aa substitution for aa 89 - 111. Over aa 264 - 437, human E2F-1 shows 89% and 80% aa identity to canine and mouse E2F-1, respectively.

Long Name

E2F Transcription Factor 1

Entrez Gene IDs

1869 (Human); 13555 (Mouse); 399489 (Rat)

Alternate Names

E2F transcription factor 1; E2F1; E2F-1; PBR3; PRB-binding protein E2F-1; RBAP1; RBAP-1; RBBP3; RBBP-3; RBP3; Retinoblastoma-associated protein 1; Retinoblastoma-binding protein 3; transcription factor E2F1