Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Western Blot, ELISA Capture

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all E2F-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human E2F-1
Val264-Phe437
Accession # Q01094

Specificity

Detects human E2F-1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human E2F-2, -3, -4, -5, -6, -7, and -8 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human E2F‑1 Antibody

E2F‑1 antibody in Human Placenta by Immunohistochemistry (IHC-P).

E2F‑1 in Human Placenta.

E2F‑1 was detected in immersion fixed paraffin-embedded sections of human placenta using 5 µg/mL Sheep Anti-Human E2F‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4825) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human E2F‑1 antibody by Western Blot.

Detection of Human E2F‑1 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and human skin tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human E2F-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4825) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for E2F-1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human E2F‑1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human placenta

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and human skin tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: E2F-1

E2F-1 (adenovirus E2 gene promoter region binding factor 1; also retinoblastoma [RB]-binding protein 3/RBP3) is a 57 - 60 kDa nuclear member of the E2F/DP family of transcription factors. It has a dual function, mediating both cell proliferation and apoptosis in a context-dependent manner. Human E2F-1 is 437 amino acids (aa) in length. It contains a cyclin A: CDK2 binding site (aa 67 - 108), a DNA-binding region (aa 110 - 194), and an RB protein binding motif (aa 409 - 426) that, when engaged, inactivates E2F-1. There is one potential in-frame 23 aa substitution for aa 89 - 111. Over aa 264 - 437, human E2F-1 shows 89% and 80% aa identity to canine and mouse E2F-1, respectively.

Long Name

E2F Transcription Factor 1

Alternate Names

E2F1, PBR3, RBAP-1, RBBP-3, RBP3

Entrez Gene IDs

1869 (Human); 13555 (Mouse); 399489 (Rat)

Gene Symbol

E2F1

UniProt

Q01094

Additional E2F-1 Products

Product Documents for Human E2F‑1 Antibody

Certificate of Analysis

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Product Specific Notices for Human E2F‑1 Antibody

For research use only

Citations for Human E2F‑1 Antibody

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Protocols

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