|E2F‑1 in Human Placenta. E2F‑1 was detected in immersion fixed paraffin-embedded sections of human placenta using 5 µg/mL Sheep Anti-Human E2F‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4825) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.|
|Detection of Human E2F‑1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and human skin tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human E2F‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4825) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for E2F‑1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.|
E2F-1 (adenovirus E2 gene promoter region binding factor 1; also retinoblastoma [RB]-binding protein 3/RBP3) is a 57 - 60 kDa nuclear member of the E2F/DP family of transcription factors. It has a dual function, mediating both cell proliferation and apoptosis in a context-dependent manner. Human E2F-1 is 437 amino acids (aa) in length. It contains a cyclin A: CDK2 binding site (aa 67 - 108), a DNA-binding region (aa 110 - 194), and an RB protein binding motif (aa 409 - 426) that, when engaged, inactivates E2F-1. There is one potential in-frame 23 aa substitution for aa 89 - 111. Over aa 264 - 437, human E2F-1 shows 89% and 80% aa identity to canine and mouse E2F-1, respectively.
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