Human EDA2R/TNFRSF27/XEDAR Antibody

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Product Details
Supplemental Products

Human EDA2R/TNFRSF27/XEDAR Antibody Summary

Species Reactivity
Detects human EDA2R/TNFRSF27/XEDAR in direct ELISAs and Western blots.
Monoclonal Mouse IgG1 Clone # 173111
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human EDA2R/TNFRSF27/XEDAR
Accession # Q9HAV5
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.


Recommended Concentration
Western Blot
1 µg/mL
Recombinant Human EDA2R/TNFRSF27/XEDAR Fc Chimera (Catalog # 1093-XD)
Blockade of Receptor-ligand Interaction
In a functional ELISA, 1-3 µg/mL of this antibody will block 50% of the binding of 5 ng/mL of Recombinant Human EDA-A2 (Catalog # 922-ED) to immobilized Recombinant Human EDA2R/TNFRSF27/XEDAR Fc Chimera (Catalog # 1093-XD) coated at 1 µg/mL (100 µL/well). At 20 μg/mL, this antibody will block >90% of the binding.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EDA2R/TNFRSF27/XEDAR

X-linked Ectodysplasin Receptor (XEDAR), also known as EDA2R and TNFRSF27, is an approximately 45 kDa transmembrane protein in the TNF receptor superfamily (1). Mature human XEDAR consists of a 136 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 140 aa cytoplasmic domain (2). Within the ECD, human XEDAR shares 87% aa sequence identity with mouse and rat XEDAR. A 55 kDa long isoform of human XEDAR carries a 21 aa insertion in the juxtamembrane cytoplasmic domain (3). A 20 kDa fragment of the ECD can be shed by metalloprotease mediated cleavage (4). XEDAR binds selectively to the EDA-A2 variant of Ectodysplasin (EDA), while the closely related receptor EDAR binds selectively to the EDA-A1 variant (2). Other than a 2 aa deletion in its TNF-like domain, EDA-A2 is identical to EDA-A1 (2). Mutations in both EDAR and EDA are associated with hypohidrotic ectodermal dysplasia (HED), a disorder of hair, tooth, and eccrine sweat gland morphogenesis (5). XEDAR itself is strongly associated with androgenetic alopecia (male hair loss) (6). XEDAR is widely expressed, notably in embryonic basal epidermal cells and maturing hair follicles (2, 7, 8). Even though it does not contain a cytoplasmic death domain, XEDAR can associate with Fas and induce EDA-A2 dependent apoptosis (7, 9). Its transcription is directly induced by p53, and XEDAR mediated cell death is p53 dependent (7, 10). XEDAR is down‑regulated in breast, colon, and lung cancers, particularly in cases with p53 mutations (7, 11). XEDAR also plays a role in EDA‑A2 induced skeletal muscle degeneration and osteoblast differentiation (8, 12).

  1. Pfeffer, K. (2003) Cytokine Growth Factor Rev. 14:185.
  2. Yan, M. et al. (2000) Science 290:523.
  3. Sinha, S.K. et al. (2002) J. Biol. Chem. 277:44953.
  4. Tanikawa, C. et al. (2010) Mol. Cancer Res. 8:855.
  5. Mikkola, M.L. (2009) Am. J. Med. Genet. 149A:2031.
  6. Prodi, D.A. et al. (2008) J. Invest. Dermatol. 128:2268.
  7. Tanikawa, C. et al. (2009) Oncogene 28:3081.
  8. Newton, K. et al. (2004) Mol. Cell. Biol. 24:1608.
  9. Sinha, S.K. and P.M. Chaudhary (2004) J. Biol. Chem. 279:41873.
  10. Brosh, R. et al. (2010) FEBS Lett. 584:2473.
  11. Punj, V. et al. (2010) Clin. Cancer Res. 16:1140.
  12. Chang, B. et al. (2007) Cancer Gene Ther. 14:927.
Long Name
Ectodysplasin A2 Receptor
Entrez Gene IDs
60401 (Human); 245527 (Mouse); 710244 (Rhesus Macaque)
Alternate Names
ectodysplasin A2 receptor; EDA2R; EDA-A2 receptor; EDAA2R; EDA-A2R; EDAA2Rtumor necrosis factor receptor superfamily member XEDAR; EDAR2; TNFRSF27; XEDAR; XEDARTNFRSF27tumor necrosis factor receptor superfamily member 27; X-linked ectodysplasin-A2 receptor

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