Human EMR3 PE-conjugated Antibody

Recombinant Monoclonal Antibody

Discontinued Product

FAB8496P has been discontinued.
Detection  of EMR3 in Human Peripheral Blood Monocytes by Flow Cytometry.
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Human EMR3 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Stains human EMR3 transfected but not irrelevant transfected cells in flow cytometry.
Source
Recombinant Monoclonal Mouse IgG2B Clone # 908235
Purification
Protein A or G purified from cell culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human EMR3
Accession # NP_115960
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection  of EMR3 in Human Peripheral Blood Monocytes by Flow Cytometry. View Larger

Detection of EMR3 in Human Peripheral Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human EMR3 PE‑conjugated Monoclonal Antibody (Catalog # FAB8496P) or (B) Mouse IgG2B Phycoerythrin Isotype Control (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins

Flow Cytometry Detection of EMR3 in HEK293 Human Cell Line Transfected with Human EMR3 and eGFP by Flow Cytometry. View Larger

Detection of EMR3 in HEK293 Human Cell Line Transfected with Human EMR3 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human EMR3 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human EMR3 PE‑conjugated Monoclonal Antibody (Catalog # FAB8496P). Quadrant markers were set based on control antibody staining (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: EMR3

EMR3 is a member of the class B seven-span transmembrane (TM7) receptor family. It is expressed predominantly by cells of the immune system, with highest levels in neutrophils, monocytes and macrophages. It may play a role in myeloid-myeloid interactions during immune and inflammatory responses. EMR3 upregulation in glioblastoma multiforme (GBM) is associated with poor survival and may be a potential therapeutic target.

Long Name
EGF-like Module Containing, Mucin-like, Hormone Receptor-like 3
Entrez Gene IDs
84658 (Human)
Alternate Names
egf-like module containing, mucin-like, hormone receptor-like 3; EGF-like module receptor 3; EGF-like module-containing mucin-like hormone receptor-like 3; egf-like module-containing mucin-like receptor 3; EMR3

Product Datasheets

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