Human EMR3 PE-conjugated Antibody: R&D Systems

Human EMR3 PE-conjugated Antibody

FAB8496P has been discontinued. View all EMR3 products.
Recombinant Monoclonal Antibody.
  
  • Species Reactivity
    Human
  • Specificity
    Stains human EMR3 transfected but not irrelevant transfected cells in flow cytometry.
  • Source
    Recombinant Monoclonal Mouse IgG2B Clone # 908235
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human EMR3
    Accession # NP_115960
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of EMR3 in Human Peripheral Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human EMR3 PE‑conjugated Monoclonal Antibody (Catalog # FAB8496P) or (B) Mouse IgG2B Phycoerythrin Isotype Control (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins
Detection of EMR3 in HEK293 Human Cell Line Transfected with Human EMR3 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human EMR3 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human EMR3 PE‑conjugated Monoclonal Antibody (Catalog # FAB8496P). Quadrant markers were set based on control antibody staining (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: EMR3
EMR3 is a member of the class B seven-span transmembrane (TM7) receptor family. It is expressed predominantly by cells of the immune system, with highest levels in neutrophils, monocytes and macrophages. It may play a role in myeloid-myeloid interactions during immune and inflammatory responses. EMR3 upregulation in glioblastoma multiforme (GBM) is associated with poor survival and may be a potential therapeutic target.
  • Long Name:
    EGF-like Module Containing, Mucin-like, Hormone Receptor-like 3
  • Entrez Gene IDs:
    84658 (Human)
  • Alternate Names:
    egf-like module containing, mucin-like, hormone receptor-like 3; EGF-like module receptor 3; EGF-like module-containing mucin-like hormone receptor-like 3; egf-like module-containing mucin-like receptor 3; EMR3
Related Research Areas

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