Human Endostatin Quantikine ELISA Kit

(15 citations)   
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (50 uL)
  • Sensitivity
    0.063 ng/mL
  • Assay Range
    0.3 - 10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva)
  • Specificity
    Natural and recombinant human Endostatin
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
Product Summary
The Quantikine Human Endostatin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Endostatin in cell culture supernates, serum, plasma, and saliva. It contains E. coli-expressed recombinant human Endostatin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Endostatin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Endostatin.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva
Intra-Assay Precision Inter-Assay Precision
Standard Deviation0.


The recovery of Endostatin spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 101 91-114
EDTA Plasma (n=4) 100 92-110
Heparin Plasma (n=4) 98 86-108
Serum (n=4) 100 93-110
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Endostatin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Endostatin Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Endostatin
Endostatin is a 20 kDa proteolytic fragment of the C-terminal, non-collagenous (NC1) domain of type XVIII Collagen. It was originally identified as a factor produced by murine hemangioendothelioma cells that could specifically inhibit endothelial cell proliferation and angiogenesis. Although the molecular signals that trigger the release of Endostatin from type XVIII Collagen are not well understood, multiple proteases have been suggested to be involved in its generation including Cathepsins S, B, L, and V, Elastase, and matrix metalloproteinases (MMPs)-2, -7, and -9. Endostatin is of particular interest as it has been shown to inhibit the growth of many primary and metastatic tumors. It may also be involved in down-regulating angiogenesis during physiological processes such as wound healing and the establishment of placental circulation. The anti-angiogenic activity of Endostatin is attributable to its ability to inhibit endothelial cell proliferation and suppress VEGF-and FGF basic-induced endothelial cell migration and adhesion. Many of these effects are thought to be mediated by interactions between Endostatin and endothelial cell-expressed Transglutaminase 2, Heparin, and Integrins alpha 5 beta 1 and alpha V beta 3.
    • Entrez Gene IDs
      80781 (Human); 12822 (Mouse);
    • Alternate Names
      antiangiogenic agent; COL18A1; collagen alpha-1(XVIII) chain; collagen, type XVIII, alpha 1; FLJ27325; FLJ34914; human type XVIII collagen10endostatin; KNO; KNO1MGC74745; Knobloch syndrome, type 1; KS; multi-functional protein MFP;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 10 of 15
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    Sample Type
    1. Systemic Sclerosis Sera Impair Angiogenic Performance of Dermal Microvascular Endothelial Cells: Therapeutic Implications of Cyclophosphamide.
      Authors: Borghini A, Manetti M, Nacci F, Bellando-Randone S, Guiducci S, Matucci-Cerinic M, Ibba-Manneschi L, Weber E
      PLoS ONE, 2015;10(6):e0130166.
      Species: Human
      Sample Type: Serum
    2. Arterial Hypertension Is Characterized by Imbalance of Pro-Angiogenic versus Anti-Angiogenic Factors.
      Authors: Marek-Trzonkowska N, Kwieczynska A, Reiwer-Gostomska M, Kolinski T, Molisz A, Siebert J
      PLoS ONE, 2015;10(5):e0126190.
      Species: Human
      Sample Type: Serum
    3. Exercise increases serum endostatin levels in female and male patients with diabetes and controls.
      Authors: Sponder M, Dangl D, Kampf S, Fritzer-Szekeres M, Strametz-Juranek J
      Cardiovasc Diabetol, 2014;13(0):6.
      Species: Human
      Sample Type: Serum
    4. Antitumor activities of human placenta-derived mesenchymal stem cells expressing endostatin on ovarian cancer.
      Authors: Zheng, Lan, Zhang, Dongmei, Chen, Xianchen, Yang, Li, Wei, Yuquan, Zhao, Xia
      PLoS ONE, 2012;7(7):e39119.
      Species: Human
      Sample Type: Cell Culture Supernates
    5. In vitro vascular tube formation testing as a tool for treatment individualisation in patients with cervical cancer.
      Authors: Landt S, Heidecke H, Korlach S, Reuter C, Schwidde I, Barinoff J, Thill M, Sehouli J, Kummel S
      Anticancer Res., 2011;31(8):2609-15.
      Species: Human
      Sample Type: Serum
    6. Prognostic significance of the angiogenic factors angiogenin, endoglin and endostatin in cervical cancer.
      Authors: Landt S, Mordelt K, Schwidde I, Barinoff J, Korlach S, Stoblen F, Lichtenegger W, Sehouli J, Kummel S
      Anticancer Res., 2011;31(8):2651-5.
      Species: Human
      Sample Type: Serum
    7. Non-viral endostatin plasmid transfection of mesenchymal stem cells via collagen scaffolds.
      Authors: Sun XD, Jeng L, Bolliet C, Olsen BR, Spector M
      Biomaterials, 2009;30(6):1222-31.
      Species: Goat
      Sample Type: Cell Culture Supernates
    8. Gene transfer of matrix metalloproteinase-9 induces tumor regression of breast cancer in vivo.
      Authors: Bendrik C, Robertson J, Gauldie J, Dabrosin C
      Cancer Res., 2008;68(9):3405-12.
      Species: Human
      Sample Type: Tissue Homogenates
    9. Tamoxifen and aromatase inhibitors differentially affect vascular endothelial growth factor and endostatin levels in women with breast cancer.
      Authors: Holmes CE, Huang JC, Pace TR, Howard AB, Muss HB
      Clin. Cancer Res., 2008;14(10):3070-6.
      Species: Human
      Sample Type: Plasma
    10. Expression pattern and circulating levels of endostatin in patients with pancreas cancer.
      Authors: Ohlund D, Ardnor B, Oman M, Naredi P, Sund M
      Int. J. Cancer, 2008;122(12):2805-10.
      Species: Human
      Sample Type: Plasma
    11. Suppression of renal cell carcinoma growth and metastasis with sustained antiangiogenic gene therapy.
      Authors: Mellon MJ, Bae KH, Steding CE, Jimenez JA, Kao C, Gardner TA
      Hum. Gene Ther., 2008;19(5):487-95.
      Species: Human
      Sample Type: Serum
    12. Pharmacokinetic and pharmacodynamic study of intratumoral injection of an adenovirus encoding endostatin in patients with advanced tumors.
      Authors: Li HL, Li S, Shao JY, Lin XB, Cao Y, Jiang WQ, Liu RY, Zhao P, Zhu XF, Zeng MS, Guan ZZ, Huang W
      Gene Ther., 2007;15(4):247-56.
      Species: Human
      Sample Type: Serum
    13. Circulating endothelial cells and angiogenic serum factors during neoadjuvant chemotherapy of primary breast cancer.
      Authors: Furstenberger G, von Moos R, Lucas R, Thurlimann B, Senn HJ, Hamacher J, Boneberg EM
      Br. J. Cancer, 2006;94(4):524-31.
      Species: Human
      Sample Type: Serum
    14. Over-expression of p53 mutants in LNCaP cells alters tumor growth and angiogenesis in vivo.
      Authors: Perryman LA, Blair JM, Kingsley EA, Szymanska B, Ow KT, Wen VW, MacKenzie KL, Vermeulen PB, Jackson P, Russell PJ
      Biochem. Biophys. Res. Commun., 2006;345(3):1207-14.
      Species: Human
      Sample Type: Cell Culture Supernates
    15. Celecoxib up-regulates the expression of the zeta chain of T cell receptor complex in tumor-infiltrating lymphocytes in human cervical cancer.
      Authors: Ferrandina G, Ranelletti FO, Legge F, Salutari V, Martinelli E, Fattorossi A, Lorusso D, Zannoni G, Vellone V, Paglia A, Scambia G
      Clin. Cancer Res., 2006;12(7):2055-60.
      Species: Human
      Sample Type: Serum
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    ELISA Controls
    Description Application Cat# Citations Images  

    Quantikine Immunoassay Control Set 860 for Human Endostatin

    Ctrl QC81
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    Supplemental ELISA Products
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    Quantikine Wash Buffer 1

    ELISA WA126 5
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