Human Endothelin-1 QuantiGlo ELISA Kit

R&D Systems | Catalog # QET00B

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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Urine (100 µL)

Sensitivity

0.102 pg/mL

Assay Range

0.34-250 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Urine)
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Product Summary for Human Endothelin-1 QuantiGlo ELISA Kit

The QuantiGlo Human Endothelin-1 Chemiluminescent Immunoassay is a 4.5 hour solid phase ELISA designed to measure human Endothelin-1 levels in cell culture supernates, serum, EDTA plasma, and urine without extraction. It contains synthetic human Endothelin-1 and antibodies raised against the synthetic factor. This immunoassay has been shown to accurately quantitate human Endothelin-1. Results obtained using natural human Endothelin-1 showed dose curves that were parallel to the standard curves obtained using the QuantiGlo kit standards. These results indicate that this kit can be used to determine relative mass values of natural human Endothelin-1.

Product Specifications

Measurement

Quantitative ELISA

Detection Method

Chemiluminescent - Luminometer (Luminol)

Conjugate

HRP

Species

Human

Specificity

Natural and synthetic human Endothelin-1

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules. Cross-species reactivity not tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, EDTA Plasma, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 1.78 20.5 90.9 1.76 19.8 86.8
Standard Deviation 0.06 0.7 2.4 0.16 1.3 3.9
CV% 3.4 3.4 2.6 9.1 6.6 4.5

Recovery for Human Endothelin-1 QuantiGlo ELISA Kit

The recovery of synthetic human Endothelin-1 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 101 93-107
EDTA Plasma (n=4) 94 88-99
Serum (n=4) 95 87-103
Urine (n=4) 98 92-105

Linearity

To assess the linearity of the assay, samples containing or spiked with high concentrations of Endothelin-1 in various matrices were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human Endothelin-1 Chemiluminescent ELISA Linearity

Scientific Data Images for Human Endothelin-1 QuantiGlo ELISA Kit

Human Endothelin-1 Chemiluminescent ELISA Standard Curve

Human Endothelin-1 Chemiluminescent ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Endothelin-1

Endothelin-1 (ET-1), a peptide of 21 amino acid (aa) residues, is a pleiotropic molecule best known for its action as a potent vasoconstrictor (1). Originally isolated from porcine aortic endothelial cells, ET-1 is one of a family of three proteins encoded by distinct genes that also includes Endothelin-2 (ET-2) and Endothelin-3 (ET-3) (2, 3). ET-2 and ET-3 differ from ET-1 by 2 and 6 amino acids, respectively (1, 2). All members of the Endothelin family contain two essential disulfide bridges and six conserved aa residues at the C-terminus. Human ET-1 is initially synthesized as a pre-pro-polypeptide of 212 amino acids (2, 4). It is proteolytically cleaved by a signal peptidase to produce pro-ET-1 and further processed by a Furin-like protease to yield a 38 aa peptide termed Big ET-1 (5, 6). Big ET-1 is then cleaved by the membrane-bound metalloprotease Endothelin-converting enzyme (ECE-1), producing the potent 21 aa mature form ET-1 (aa 1-21) (7, 8). Alternatively, ET-1 may exist in an active 31 aa form (ET-1 (aa 1-31)) following cleavage of Big ET-1 by chymase (9-12). The vascular endothelium is an abundant source of ET-1 (3, 13). It may also be expressed by leukocytes, smooth muscle cells, mesangial cells, cardiac myocytes, and astrocytes (14, 15). ET-1 can be induced in endothelial cells by many factors including mechanical stimulation, various hormones, and pro-inflammatory cytokines (16). Production is inhibited by nitric oxide (NO), Prostacyclin, and atrial natriuretic peptide (ANP) (17-19). 
Two receptors for the Endothelin family have been cloned and designated ETA and ETB (20-23). ETA and ETB belong to the large family of heptahelical G protein-coupled receptors. The ETA receptor shows a higher affinity for ET-1 than for ET-2 and lowest affinity for ET-3, while the ETB receptor shows approximately equal affinity for each of the three Endothelins (21, 22, 24). ETA is primarily responsible for the vasoconstrictor effects of ET-1 and is expressed by blood vessel smooth muscle cells (25, 26). The ETB receptor is also present in smooth muscle and the endothelia of blood vessels, kidney, lung, and brain (27). ET-1 has the ability to activate an array of signaling cascades including classical phosphatidylinositol turnover pathways leading to downstream PKC activation and Ca2+ mobilization (28-32). Other potential signaling mediators activated or produced by ET-1 include PI 3-kinase/Akt, NO, FAK, and Rho GTPases (32-37). ET-1 signaling may also be mediated indirectly via transactivation of the EGF receptor leading to downstream signaling by Ras and MAP kinases (38, 39). Injection of a single dose of ET-1 produces an initial decrease in systemic blood pressure followed by a prolonged increase in blood pressure (16, 40, 41). Blockade of Endothelin receptors with a systemic injection of an ETA/ETB antagonist causes progressive vasodilation, and elevated levels of ET-1 are found in some forms of human hypertension (42, 43). ET-1 also stimulates cardiac contraction and the growth of cardiac myocytes, regulates the release of vasoactive substances, and stimulates smooth muscle cell mitogenesis (32, 44-46). It also acts as a pro-survival factor for endothelial cells and regulates secretion by hypothalamic and pituitary cells (47, 48). ET-1 may control inflammatory responses by promoting the adhesion and migration of neutrophils and stimulating the production of pro-inflammatory cytokines (49-53). It has also been implicated in cancer progression at several levels including regulating the proliferation and migration of tumor cells and acting as a pro-angiogenic factor (54, 55). In addition, ET-1 has putative roles in other pathologies including septic shock, atherosclerosis, heart failure, renal insufficiency, pulmonary hypertension, and cerebrovascular conditions associated with subarachnoid hemorrhage (15, 56-63).

Alternate Names

ARCND3, EDN1, ET1, HDLCQ7, PPET1, QME

Entrez Gene IDs

1906 (Human); 13614 (Mouse); 24323 (Rat)

Gene Symbol

EDN1

Additional Endothelin-1 Products

Product Documents for Human Endothelin-1 QuantiGlo ELISA Kit

Certificate of Analysis

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Product Specific Notices for Human Endothelin-1 QuantiGlo ELISA Kit

For research use only

Citations for Human Endothelin-1 QuantiGlo ELISA Kit

Customer Reviews for Human Endothelin-1 QuantiGlo ELISA Kit (3)

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  • Human Endothelin-1 QuantiGlo ELISA Kit
    Name: Anonymous
    Sample Tested: Plasma
    Verified Customer | Posted 05/24/2019
  • Human Endothelin-1 QuantiGlo ELISA Kit
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 07/24/2017
    undiluted human serum from healthy donors
    Human Endothelin-1 QuantiGlo ELISA Kit QET00B
  • Human Endothelin-1 QuantiGlo ELISA Kit
    Name: Anonymous
    Sample Tested: EDTA Plasma
    Verified Customer | Posted 06/29/2017
    Very robust and easy to use kit.
    Human Endothelin-1 QuantiGlo ELISA Kit QET00B

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