Human Endothelin-1 QuantiGlo ELISA Kit QET00B: R&D Systems

Human Endothelin-1 QuantiGlo ELISA Kit

(26 citations)
(2 Reviews)
  
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Urine (100 uL)
  • Sensitivity
    0.102 pg/mL
  • Assay Range
    0.3 - 250 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Urine)
  • Specificity
    Natural and synthetic human Endothelin-1
  • Cross-reactivity
    Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity not tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC197, QuantiGlo Immunoassay Control Set 732 Human Endothelin-1 - Please Inquire
Product Summary
The QuantiGlo Human Endothelin-1 Chemiluminescent Immunoassay is a 4.5 hour solid phase ELISA designed to measure human Endothelin-1 levels in cell culture supernates, serum, EDTA plasma, and urine without extraction. It contains synthetic human Endothelin-1 and antibodies raised against the synthetic factor. This immunoassay has been shown to accurately quantitate human Endothelin-1. Results obtained using natural human Endothelin-1 showed dose curves that were parallel to the standard curves obtained using the QuantiGlo kit standards. These results indicate that this kit can be used to determine relative mass values of natural human Endothelin-1.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Urine
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean1.7820.590.91.7619.886.8
Standard Deviation0.060.72.40.161.33.9
CV%3.43.42.69.16.64.5

Recovery

The recovery of synthetic human Endothelin-1 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 101 93-107
EDTA Plasma (n=4) 94 88-99
Serum (n=4) 95 87-103
Urine (n=4) 98 92-105
Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of Endothelin-1 in various matrices were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Endothelin-1 QuantiGlo ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Endothelin-1
Endothelin-1 (ET-1) is produced by many tissues including lung, kidney, brain, endocrine glands, and placenta. It is also expressed by several cell types including, mast cells, endothelial cells, epithelial and smooth muscle cells. The most prominent source is vascular endothelium. ET-1 is best known as a potent vasoconstrictor. Elevated blood ET-1 levels are associated with a variety of diseases, possibly as a function of a stress response.
    • Entrez Gene IDs
      1906 (Human); 13614 (Mouse); 24323 (Rat);
    • Alternate Names
      EDN1; endothelin 1; Endothelin-1; ET1; HDLCQ7; PPET1; preproendothelin-1;
    Related Research Areas
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    26 Citations: Showing 1 - 10
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    Species
    Sample Type
    1. Endothelin receptor-specific control of endoplasmic reticulum stress and apoptosis in the kidney
      Authors: C De Miguel, WC Hamrick, JL Hobbs, DM Pollock, PK Carmines, JS Pollock
      Sci Rep, 2017;7(0):43152.
      Species: Rat
      Sample Type: Plasma
    2. Albuminuria Is Associated with Endothelial Dysfunction and Elevated Plasma Endothelin-1 in Sickle Cell Anemia
      PLoS ONE, 2016;11(9):e0162652.
      Species: Human
      Sample Type: Plasma
    3. p66Shc regulates renal vascular tone in hypertension-induced nephropathy
      Authors: Bradley Miller
      J Clin Invest, 2016;0(0):.
      Species: Rat
      Sample Type: Tissue Culture Supernates
    4. Endothelin-1 Predicts Hemodynamically Assessed Pulmonary Arterial Hypertension in HIV Infection.
      Authors: Parikh R, Ma Y, Scherzer R, Heringer A, Macgregor J, Martin J, Deeks S, Ganz P, Hsue P
      PLoS ONE, 2016;11(1):e0146355.
      Species: Human
      Sample Type: Plasma
    5. Free radical scavenging decreases endothelin-1 excretion and glomerular albumin permeability during type 1 diabetes
      Physiol Rep, 2016;4(24):.
      Species: Rat
      Sample Type: Urine
    6. Association of endothelin-1 and matrix metallopeptidase-9 with metabolic syndrome in middle-aged and older adults.
      Authors: Yu A, Tam B, Yau W, Chan K, Yu S, Chung T, Siu P
      Diabetol Metab Syndr, 2015;7(0):111.
      Species: Human
      Sample Type: Serum
    7. Optimum AT1 receptor-neprilysin inhibition has superior cardioprotective effects compared with AT1 receptor blockade alone in hypertensive rats.
      Authors: Roksnoer L, van Veghel R, de Vries R, Garrelds I, Bhaggoe U, Friesema E, Leijten F, Poglitsch M, Domenig O, Clahsen-van Groningen M, Hoorn E, Jan Danser A, Batenburg W
      Kidney Int, 2015;88(1):109-20.
    8. Chemotherapy-induced pulmonary hypertension: role of alkylating agents.
      Authors: Ranchoux B, Gunther S, Quarck R, Chaumais M, Dorfmuller P, Antigny F, Dumas S, Raymond N, Lau E, Savale L, Jais X, Sitbon O, Simonneau G, Stenmark K, Cohen-Kaminsky S, Humbert M, Montani D, Perros F
      Am J Pathol, 2015;185(2):356-71.
      Species: Rabbit
      Sample Type: Plasma
    9. Aberrant chloride intracellular channel 4 expression contributes to endothelial dysfunction in pulmonary arterial hypertension.
      Authors: Wojciak-Stothard B, Abdul-Salam V, Lao K, Tsang H, Irwin D, Lisk C, Loomis Z, Stenmark K, Edwards J, Yuspa S, Howard L, Edwards R, Rhodes C, Gibbs J, Wharton J, Zhao L, Wilkins M
      Circulation, 2014;129(17):1770-80.
      Species: Human
      Sample Type: Cell Culture Supernates
    10. The in vitro pharmacology of GS-5759, a novel bifunctional phosphodiesterase 4 inhibitor and long acting beta2-adrenoceptor agonist.
      Authors: Tannheimer S, Sorensen E, Cui Z, Kim M, Patel L, Baker W, Phillips G, Wright C, Salmon M
      J Pharmacol Exp Ther, 2014;349(1):85-93.
      Species: Human
      Sample Type: Cell Culture Supernates
    11. miR-218 regulates focal adhesion kinase-dependent TGFbeta signaling in fibroblasts.
      Authors: Guo F, Carter D, Leask A
      Mol Biol Cell, 2014;25(7):1151-8.
      Species: Human
      Sample Type: Cell Culture Supernates
    12. Gingival fibroblasts display reduced adhesion and spreading on extracellular matrix: a possible basis for scarless tissue repair?
      Authors: Guo F, Carter DE, Mukhopadhyay A, Leask A
      PLoS ONE, 2011;6(11):e27097.
      Species: Human
      Sample Type: Cell Culture Supernates
    13. Daily red wine consumption improves vascular function by a soluble guanylyl cyclase-dependent pathway.
      Authors: Botden IP, Langendonk JG, Meima ME
      Am. J. Hypertens., 2011;24(2):162-8.
      Species: Human
      Sample Type: Plasma
    14. Early life stress downregulates endothelin receptor expression and enhances acute stress-mediated blood pressure responses in adult rats.
      Authors: Loria AS, D'Angelo G, Pollock DM, Pollock JS
      Am. J. Physiol. Regul. Integr. Comp. Physiol., 2010;299(1):R185-91.
      Species: Rat
      Sample Type: Plasma
    15. Endothelin receptors blockade blunts hypoxia-induced increase in PAP in humans.
      Authors: Pham I, Wuerzner G, Richalet JP, Peyrard S, Azizi M
      Eur. J. Clin. Invest., 2010;40(3):195-202.
      Species: Human
      Sample Type: Plasma
    16. Effects of nonoral estradiol-micronized progesterone or low-dose oral estradiol-drospirenone therapy on metabolic variables and markers of endothelial function in early postmenopause.
      Authors: Casanova G, Radavelli S, Lhullier F, Spritzer PM
      Fertil. Steril., 2009;92(2):605-12.
      Species: Human
      Sample Type: Plasma
    17. Vehicular emissions induce vascular MMP-9 expression and activity associated with endothelin-1-mediated pathways.
      Authors: Lund AK, Lucero J, Lucas S, Madden MC, McDonald JD, Seagrave JC, Knuckles TL, Campen MJ
      Arterioscler. Thromb. Vasc. Biol., 2009;29(4):511-7.
      Species: Human
      Sample Type: Plasma
    18. Endothelial function, endothelin-1, and fibrinogen in young women using the vaginal contraceptive ring.
      Authors: Torgrimson BN, Meendering JR, Miller NP, Kaplan PF, Minson CT
      Fertil. Steril., 2009;92(2):441-7.
      Species: Human
      Sample Type: Serum
    19. Regulation of angiogenic factors by HDM2 in renal cell carcinoma.
      Authors: Carroll VA, Ashcroft M
      Cancer Res., 2008;68(2):545-52.
      Species: Human
      Sample Type: Cell Culture Supernates
    20. Eosinophil-derived cationic proteins activate the synthesis of remodeling factors by airway epithelial cells.
      Authors: Pegorier S, Wagner LA, Gleich GJ, Pretolani M
      J. Immunol., 2006;177(7):4861-9.
      Species: Human
      Sample Type: Cell Culture Supernates
    21. Digital blood flow and plasma endothelin concentration in clinically endotoxemic horses.
      Authors: Menzies-Gow NJ, Bailey SR, Stevens K, Katz L, Elliott J, Marr CM
      Am. J. Vet. Res., 2005;66(4):630-6.
      Species: Equine
      Sample Type: Plasma
    22. Mechanisms of elevated plasma endothelin-1 in CHF: congestion increases pulmonary synthesis and secretion of endothelin-1.
      Authors: von Lueder TG, Kjekshus H, Edvardsen T, ØIe E, Urheim S, Vinge LE, Ahmed MS, Smiseth OA, Attramadal H
      Cardiovasc. Res., 2004;63(1):41-50.
      Species: Porcine
      Sample Type: Plasma
    23. Novel endothelin receptor antagonist attenuates endotoxin-induced lung injury in sheep.
      Authors: Kuklin VN, Kirov MY, Evgenov OV, Sovershaev MA, Sjoberg J, Kirova SS, Bjertnaes LJ
      Crit. Care Med., 2004;32(3):766-73.
      Species: Ovine
      Sample Type: Plasma
    24. Renal endothelin in chronic angiotensin II hypertension.
      Authors: Sasser JM, Pollock JS, Pollock DM
      Am. J. Physiol. Regul. Integr. Comp. Physiol., 2002;283(1):R243-8.
      Species: Rat
      Sample Type: Tissue Homogenates
    25. Passive transfer of scrub typhus plasma to patients with AIDS: a descriptive clinical study.
      Authors: Watt G, Kantipong P, Jongsakul K, de Souza M, Burnouf T
      QJM, 2001;94(11):599-607.
      Species: Human
      Sample Type: Plasma
    26. Endothelin and ET(A) receptors in long-term arterial pressure homeostasis in conscious nonhuman primates.
      Authors: Reinhart GA, Preusser LC, Opgenorth TJ, Wegner CD, Cox BF
      Am. J. Physiol. Regul. Integr. Comp. Physiol., 2000;279(5):R1701-6.
      Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
      Sample Type: Plasma
    Expand to show all 26 Citations
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    QuantiGlo Immunoassay Control Set 732 Human Endothelin-1

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     Human Endothelin-1 QuantiGlo ELISA Kit QET00B
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      Anonymous 07/24/2017
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     Human Endothelin-1 QuantiGlo ELISA Kit QET00B
    : Human Endothelin-1 QuantiGlo ELISA Kit [QET00B]

    Summary

    Sample TestedSerum

    Other Experimental Details

    Other Experimental Detailsundiluted human serum from healthy donors
     Human Endothelin-1 QuantiGlo ELISA Kit QET00B
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      Anonymous 06/29/2017
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     Human Endothelin-1 QuantiGlo ELISA Kit QET00B
    : Human Endothelin-1 QuantiGlo ELISA Kit [QET00B]

    Summary

    Sample TestedEDTA Plasma

    Other Experimental Details

    Other Experimental DetailsVery robust and easy to use kit.

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