Human EpCAM/TROP‑1 Biotinylated Antibody

Catalog # Availability Size / Price Qty
Product Details
Citations (19)
Supplemental Products

Human EpCAM/TROP‑1 Biotinylated Antibody Summary

Species Reactivity
Detects human EpCAM/TROP-1 in sandwich immunoassays and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human (rh) ALCAM, rhBCAM, recombinant mouse MAdCAM-1, rhNCAM-1, and rhNCAM-L1 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant human EpCAM, extracelllular domain
Accession # CAA32870
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human EpCAM/TROP‑1 Fc Chimera (Catalog # 960-EP)
Flow Cytometry
2.5 µg/106 cells
HT‑29 human colon adenocarcinoma cell line

Human EpCAM/TROP-1 Sandwich Immunoassay

Recommended Concentration
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human
EpCAM/TROP‑1 Antibody (Catalog # MAB9601)

Standard: Recombinant Human EpCAM/TROP1 Fc Chimera Protein, CF (Catalog # 960-EP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EpCAM/TROP1

Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP-1, is a 40 kDa transmembrane glycoprotein composed of a 242 amino acid (aa) extracellular domain with two epidermal-growth-factor-like (EGF-like) repeats within the cysteine-rich N-terminal region, a 23 aa transmembrane domain, and a 26 aa cytoplasmic domain. Human and mouse EpCAM share 82% aa sequence identity. In human, EpCAM also shares 49% aa sequence homology with TROP-2/EGP-1. During embryonic development, EpCAM is detected in fetal lung, kidney, liver, pancreas, skin, and germ cells. In adults, human EpCAM is detected in basolateral cell membranes of all simple, pseudo-stratified, and transitional epithelia, but is not detected in normal squamous stratified epithelia, mesenchymal tissue, muscular tissue, neuro-endocrine tissue, or lymphoid tissue (1). EpCAM expression has been found to increase in actively proliferating epithelia tissues and during adult liver regeneration (1, 2). EpCAM expression is also found to increase in human malignant neoplasias, with most carcinoma expressing EpCAM including those of arising from squamousal epithelia (1). EpCAM has been shown function as a homophilic Ca2+ independent adhesion molecule (3). Homophilic adhesion via EpCAM requires the interaction of both EGF-like repeats, with the first EGF-like repeat mediating reciprocal interaction between EpCAM molecules on opposing cells, while the second repeat is involved in lateral interaction of EpCAM. Lateral interaction of EpCAM lead to the formation of dimers and tetramers (4). During homophilic adhesion the cytoplasmic tail of EpCAM interacts with the actin cytoskeleton via a direct association alpha -actinin (5).

  1. Balzar, M. et al. (1999) J. Mol. Med. 77:699.
  2. Boer, C.J, et al. (1999) J. Pathol. 188:201.
  3. Litvinow, S.V. et al. (1994) J. Cell Biol. 125:437.
  4. Balzar, M. et al. (2001) Mol. Cell. Biol. 21:2570.
  5. Balzar, M. et al. (1998) Mol. Cell. Biol. 18:4388.
Long Name
Epithelial Cell Adhesion Molecule
Entrez Gene IDs
4072 (Human); 17075 (Mouse); 171577 (Rat); 102123042 (Cynomolgus Monkey)
Alternate Names
17-1A; 323/A3; ACSTD1; antigen identified by monoclonal AUA1; CD326 antigen; CD326; Cell surface glycoprotein Trop-1; chromosome 4, surface marker (35kD glycoprotein); DIAR5; EGP; EGP-2; EGP314; EGP40; EpCAM; epithelial cell adhesion molecule; Epithelial cell surface antigen; Epithelial glycoprotein 314; Epithelial glycoprotein; ESA; GA733-2; GA733-2EGP34; gp40; hEGP314; HNPCC8; KS 1/4 antigen; KS1/4; KSAHEA125; M1S2; M4S1; M4S1Ly74; Major gastrointestinal tumor-associated protein GA733-2; MIC18MH99; MOC31; TACST-1; TACSTD1; TROP1; TROP1CD326; Tumor-associated calcium signal transducer 1CO-17A

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Citations for Human EpCAM/TROP‑1 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. Multifunctional Gelatin-Nanoparticle-Modified Chip for Enhanced Capture and Non-Destructive Release of Circulating Tumor Cells
    Authors: L Xu, T Ma, K Zhang, Q Zhang, M Yu, X Zhao
    Micromachines, 2022;13(3):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ELISA Development
  2. Antibody-engineered red blood cell interface for high-performance capture and release of circulating tumor cells
    Authors: H Shen, R Su, J Peng, L Zhu, K Deng, Q Niu, Y Song, L Yang, L Wu, Z Zhu, C Yang
    Bioactive materials, 2022;11(0):32-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Capture
  3. The value of circulating tumor cells with positive centromere probe 8 in the diagnosis of small pulmonary nodules
    Authors: C Liu, H Chen, T Sun, H Wang, B Chen, X Wang
    Translational Oncology, 2021;14(5):101052.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  4. High-Throughput Label-Free Isolation of Heterogeneous Circulating Tumor Cells and CTC Clusters from Non-Small-Cell Lung Cancer Patients
    Authors: M Zeinali, M Lee, A Nadhan, A Mathur, C Hedman, E Lin, R Harouaka, MS Wicha, L Zhao, N Palanisamy, M Hafner, R Reddy, GP Kalemkeria, BJ Schneider, KA Hassan, N Ramnath, S Nagrath
    Cancers (Basel), 2020;12(1):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. High-purity isolation of rare single cells from blood using a tiered microchip system
    Authors: O Gur, CL Chang, R Jain, Y Zhong, CA Savran
    PLoS ONE, 2020;15(3):e0229949.
    Species: Human
    Sample Types: Whole Blood
    Applications: ELISA Capture
  6. A temporary indwelling intravascular aphaeretic system for in vivo enrichment of circulating tumor cells
    Authors: TH Kim, Y Wang, CR Oliver, DH Thamm, L Cooling, C Paoletti, KJ Smith, S Nagrath, DF Hayes
    Nat Commun, 2019;10(1):1478.
    Species: Xenograft
    Sample Types: In Vivo
    Applications: Cell Capture
  7. Emerin deregulation links nuclear shape instability to metastatic potential
    Authors: M Reis-Sobre, JF Chen, T Novitskaya, S You, S Morley, K Steadman, N Kaur Gill, A Eskaros, M Rotinen, CY Chu, LWK Chung, H Tanaka, W Yang, BS Knudsen, HR Tseng, AC Rowat, EM Posadas, A Zijlstra, D Di Vizio, MR Freeman
    Cancer Res., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Capture
  8. Magnetic Nanowire Networks for Dual-Isolation and Detection of Tumor-Associated Circulating Biomarkers
    Authors: H Lee, M Choi, J Lim, M Jo, JY Han, TM Kim, Y Cho
    Theranostics, 2018;8(2):505-517.
    Applications: Functional Assay
  9. Tumor-Derived Exosomal Long Noncoding RNAs as Promising Diagnostic Biomarkers for Prostate Cancer
    Authors: YH Wang, J Ji, BC Wang, H Chen, ZH Yang, K Wang, CL Luo, WW Zhang, FB Wang, XL Zhang
    Cell. Physiol. Biochem., 2018;46(2):532-545.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  10. Photo and Thermo Dual-Responsive Copolymer Surfaces for Efficient Cell Capture and Release
    Authors: Y Hao, H Liu, G Li, H Cui, L Jiang, S Wang
    Chemphyschem, 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  11. Enhanced Isolation and Release of Circulating Tumor Cells Using Nanoparticle Binding and Ligand Exchange in a Microfluidic Chip
    Authors: MH Park, E Reátegui, W Li, SN Tessier, KH Wong, AE Jensen, V Thapar, D Ting, M Toner, SL Stott, PT Hammond
    J. Am. Chem. Soc., 2017;139(7):2741-2749.
    Species: Human
    Sample Types: Whole Cells
    Applications: Microfluidic Assay
  12. Increased expression of long-noncoding RNA ZFAS1 is associated with epithelial mesenchymal transition of gastric cancer
    Aging (Albany NY), 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ELISA Development (Detection)
  13. Circulating tumour cells as a biomarker for diagnosis and staging in pancreatic cancer
    Authors: J S Ankeny
    Br J Cancer, 2016;114(12):1367-75.
    Species: Human
    Sample Types: Whole Cells
    Applications: NanoVelcro Chip
  14. Soluble EpCAM levels in ascites correlate with positive cytology and neutralize catumaxomab activity in vitro.
    Authors: Seeber A, Martowicz A, Spizzo G, Buratti T, Obrist P, Fong D, Gastl G, Untergasser G
    BMC Cancer, 2015;15(0):372.
    Species: Human
    Sample Types: BALF
    Applications: ELISA Development (Detection)
  15. Urinary EpCAM in urothelial bladder cancer patients: characterisation and evaluation of biomarker potential.
    Authors: Bryan, R T, Shimwell, N J, Wei, W, Devall, A J, Pirrie, S J, James, N D, Zeegers, M P, Cheng, K K, Martin, A, Ward, D G
    Br J Cancer, 2014;110(3):679-85.
    Species: Human
    Sample Types: Urine
    Applications: Immunoprecipitation
  16. Molecular biomarker analyses using circulating tumor cells.
    Authors: Punnoose EA, Atwal SK, Spoerke JM
    PLoS ONE, 2010;5(9):e12517.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Circulating tumor cells are transcriptionally similar to the primary tumor in a murine prostate model.
    Authors: Helzer KT, Barnes HE, Day L, Harvey J, Billings PR, Forsyth A
    Cancer Res., 2009;69(19):7860-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunoprecipitation
  18. High aldehyde dehydrogenase activity: a novel functional marker of murine prostate stem/progenitor cells.
    Authors: Burger PE, Gupta R, Xiong X, Ontiveros CS, Salm SN, Moscatelli D, Wilson EL
    Stem Cells, 2009;27(9):2220-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. Isolation of rare circulating tumour cells in cancer patients by microchip technology.
    Authors: Nagrath S, Sequist LV, Maheswaran S, Bell DW, Irimia D, Ulkus L, Smith MR, Kwak EL, Digumarthy S, Muzikansky A, Ryan P, Balis UJ, Tompkins RG, Haber DA, Toner M
    Nature, 2007;450(7173):1235-9.
    Species: Human
    Sample Types: Whole Cells


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