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Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Alexa Fluor® 488-conjugated Antibody

FAB9589G Datasheet / COA / SDS
Recombinant Monoclonal Antibody For Research Use Only
Catalog # Availability Size / Price Qty
FAB9589G-100UG
Detection of ErbB2/Her2 in MCF-7 human cell line by Flow Cytometry.
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human ErbB2/Her2 in direct ELISAs. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Trastuzumab. This product is for research use only.
Source
Recombinant Monoclonal Human IgG1 Clone # Hu5
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Human ErbB2/Her2
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below
Immunocytochemistry
5-25 µg/mL
Immersion fixed SK‑BR‑3 human breast cancer cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of ErbB2/Her2 antibody in MCF-7 human cell line antibody by Flow Cytometry. View Larger

Detection of ErbB2/Her2 in MCF-7 human cell line by Flow Cytometry. MCF-7 human cell line was stained with Human Anti-Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB9589G, filled histogram) or irrelevant antibody (open histogram). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry View Larger

ErbB2/Her2 in SK‑BR‑3 Human Cell Line. ErbB2/Her2 was detected in immersion fixed SK‑BR‑3 human breast cancer cell line using Human Anti-Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB9589G, green) at 5 µg/mL for 3 hours at room temperature. Cells were counterstained with DAPI (blue). Specific staining was localized to cell membrane and cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: ErbB2/Her2

Trastuzumab is a biosimilar directed against the extracellular domain of Her2 (ErbB1). This antibody can be used in flow cytometry or various assay formats to measure Her2 expression.

Long Name
Receptor Tyrosine Protein Kinase ErbB2
Entrez Gene IDs
2064 (Human); 13866 (Mouse); 24337 (Rat); 102146608 (Cynomolgus Monkey)
Alternate Names
CD340 antigen; CD340; c-erb B2/neu protein; EC 2.7.10; EGFR2; ErbB2; HER2; HER-2; HER2EC 2.7.10.1; herstatin; Metastatic lymph node gene 19 protein; MLN 19; MLN19; Neu Oncogene; NEUHER-2/neu; neuroblastoma/glioblastoma derived oncogene homolog; NGL; NGLTKR1; p185erbB2; Proto-oncogene c-ErbB-2; Proto-oncogene Neu; receptor tyrosine-protein kinase erbB-2; TKR1; Tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog); v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)

Product Datasheets

Product Datasheet
COA
SDS

FAQs

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