Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Alexa Fluor® 488-conjugated Antibody
Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Alexa Fluor® 488-conjugated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of ErbB2/Her2 in MCF-7 human cell line by Flow Cytometry. MCF-7 human cell line was stained with Human Anti-Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB9589G, filled histogram) or irrelevant antibody (open histogram). View our protocol for Staining Membrane-associated Proteins.

ErbB2/Her2 in SK‑BR‑3 Human Cell Line. ErbB2/Her2 was detected in immersion fixed SK‑BR‑3 human breast cancer cell line using Human Anti-Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB9589G, green) at 5 µg/mL for 3 hours at room temperature. Cells were counterstained with DAPI (blue). Specific staining was localized to cell membrane and cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: ErbB2/Her2
Trastuzumab is a biosimilar directed against the extracellular domain of Her2 (ErbB1). This antibody can be used in flow cytometry or various assay formats to measure Her2 expression.
Product Datasheets
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