ErbB2/Her2 (Research Grade Trastuzumab
Biosimilar) Biotinylated Antibody

R&D Systems | Catalog # FAB9589B

Recombinant Monoclonal Antibody For Research Use Only
R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry

Label

Biotin

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # Hu5
View all ErbB2/Her2 Primary Antibodies »

Product Specifications

Immunogen

Human ErbB2/Her2

Specificity

Detects human ErbB2/Her2 in direct ELISAs. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Trastuzumab. This product is for research use only.

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Scientific Data Images

Detection of ErbB2/Her2 antibody in MCF-7 human cell line antibody by Flow Cytometry.

Detection of ErbB2/Her2 in MCF-7 human cell line by Flow Cytometry.

MCF-7 human cell line was stained with Biotinylated Human Anti-Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Monoclonal Antibody (Catalog # FAB9589B, filled histogram) or irrelevant antibody (open histogram) followed by APC-conjugated Streptavidin (Catalog # F0050). View our protocol for Staining Membrane-associated Proteins.
ErbB2/Her2 antibody in SK-BR-3 Human Cell Line by Immunocytochemistry (ICC).

ErbB2/Her2 in SK-BR-3 Human Cell Line.

ErbB2/Her2 was detected in both live and fixed SK‑BR‑3 human breast cancer cell line and but not detected in fixed U937 human histiocytic lymphoma cell line. SK-BR-3 live cells were incubated for 1 hour at 4°C with Anti-Human ErbB2/Her2 Biotinylated Monoclonal Antibody (FAB9589B) diluted at 5 µg/mL with HBSS. SK-BR-3 and U937 fixed cells were incubated for 2 hours at room temperature with Anti-Human ErbB2/Her2 Biotinylated Monoclonal Antibody (FAB9589B) diluted with antibody diluent. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; NL999) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: MCF-7 Human cell line

Immunocytochemistry

5-25 µg/mL
Sample: Live and fixed SK-BR-3 human breast cancer cell line

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: ErbB2/Her2

Trastuzumab is a biosimilar directed against the extracellular domain of Her2 (ErbB1). The biotinylated antibody can be used in flow cytometry or various assay format to measure Her2 expression and can be detected by conjugated streptavidin.

Long Name

Receptor Tyrosine Protein Kinase ErbB2

Alternate Names

CD340, HER2, Neu Oncogene, NGL, TKR1

Entrez Gene IDs

2064 (Human); 13866 (Mouse); 24337 (Rat); 102146608 (Cynomolgus Monkey)

Gene Symbol

ERBB2

Additional ErbB2/Her2 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices

For research use only

Citations for ErbB2/Her2 (Research Grade Trastuzumab
Biosimilar) Biotinylated Antibody

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Protocols

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