Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Biotin

Antibody Source

Monoclonal Mouse IgG2A Clone # AE7A5
View all Erythropoietin/EPO Primary Antibodies »

Product Specifications

Immunogen

Erythropoietin/EPO synthetic peptide analog
Ala28-Thr53
Accession # P01588

Specificity

Recognizes an epitope within the first 26 amino acids at the NH2 terminus of human urinary and human recombinant EPO. Binds human and rat EPO but does not bind sheep, baboon, or dog EPO (1, 2).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human Erythropoietin/EPO Biotinylated Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human Erythropoietin/EPO (Ultrapure) (Catalog # 266-EP)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Background: Erythropoietin/EPO

EPO, a glycoprotein produced primarily by the kidney, is the principal factor that regulates erythropoiesis by stimulating the proliferation and differentiation of erythroid progenitor cells.

References

  1. Sue and Sytkowski (1983) Proc. Natl. Acad. Sci. USA 80:3651.
  2. Sytkowski and Fisher (1985) J. Biol. Chem. 260:14727.
  3. Martinelli et al. (1985) Clin. Res. 33:349A.

Alternate Names

ECYT5, EPO, MVCD2

Entrez Gene IDs

2056 (Human); 13856 (Mouse); 24335 (Rat)

Gene Symbol

EPO

UniProt

P01588

Additional Erythropoietin/EPO Products

Product Documents for Human Erythropoietin/EPO Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Erythropoietin/EPO Biotinylated Antibody

For research use only

Citations for Human Erythropoietin/EPO Biotinylated Antibody

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