Human Erythropoietin/EPO Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAM2871-100

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Human Erythropoietin/EPO Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Recognizes an epitope within the first 26 amino acids at the NH2 terminus of human urinary and human recombinant EPO. Binds human and rat EPO but does not bind sheep, baboon, or dog EPO (1, 2).
Source
Monoclonal Mouse IgG2A Clone # AE7A5
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Erythropoietin/EPO synthetic peptide analog
Ala28-Thr53
Accession # P01588
Formulation
Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Erythropoietin/EPO (Ultrapure) (Catalog # 266-EP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Background: Erythropoietin/EPO

EPO, a glycoprotein produced primarily by the kidney, is the principal factor that regulates erythropoiesis by stimulating the proliferation and differentiation of erythroid progenitor cells.

References
  1. Sue and Sytkowski (1983) Proc. Natl. Acad. Sci. USA 80:3651.
  2. Sytkowski and Fisher (1985) J. Biol. Chem. 260:14727.
  3. Martinelli et al. (1985) Clin. Res. 33:349A.
Entrez Gene IDs
2056 (Human); 13856 (Mouse); 24335 (Rat)
Alternate Names
ECYT5; EP; EPO; epoetin; Erythropoietin; MGC138142; MVCD2

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