Human Erythropoietin/EPO Antibody

Catalog # Availability Size / Price Qty
MAB2871-SP
MAB2871-100
MAB2871-500
Product Details
Citations (12)
FAQs
Supplemental Products
Reviews (1)

Human Erythropoietin/EPO Antibody Summary

Species Reactivity
Human
Specificity
Recognizes an epitope within the first 26 amino acids at the NH2 terminus of human urinary and human recombinant EPO. Binds human and rat EPO but does not bind sheep, baboon, or dog EPO (1, 2).
Source
Monoclonal Mouse IgG2A Clone # AE7A5
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Erythropoietin/EPO synthetic peptide analog
Ala28-Thr53
Accession # P01588
Formulation
Supplied as a 0.2 μm filtered solution in PBS. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Erythropoietin/EPO (Ultrapure) (Catalog # 286-EP)
Immunoaffinity Purification
This antibody can be used with optimal binding of human EPO at pH 7-9 (1-3).
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Background: Erythropoietin/EPO

Erythropoietin (EPO) a glycoprotein produced primarily by the kidney, is the principal factor that regulates erythropoiesis by stimulating the proliferation and differentiation of erythroid progenitor cells.

Entrez Gene IDs
2056 (Human); 13856 (Mouse); 24335 (Rat)
Alternate Names
ECYT5; EP; EPO; epoetin; Erythropoietin; MGC138142; MVCD2

Product Datasheets

Citations for Human Erythropoietin/EPO Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Modified recombinant human erythropoietin with potentially reduced immunogenicity
    Authors: T Susantad, M Fuangthong, K Tharakaram, P Tit-Oon, M Ruchirawat, R Sasisekhar
    Scientific Reports, 2021;11(1):1491.
    Species: Human
    Sample Types: Protein
    Applications: ELISA Capture
  2. Physicochemical and biological evaluation of JR-131 as a biosimilar to a long-acting erythropoiesis-stimulating agent darbepoetin alfa
    Authors: J Tani, Y Ito, S Tatemichi, M Yamakami, T Fukui, Y Hatano, S Kakimoto, A Kotani, A Sugimura, K Mihara, R Yamamoto, N Tanaka, K Minami, K Takahashi, T Hirato
    PLoS ONE, 2020;15(4):e0231830.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  3. In vivo modification of the goat mammary gland glycosylation pathway
    Authors: MJ Leiva-Carr, S Jiménez-Ch, DJ Harvey, NC Parra, KC Tavares, F Camacho, A González, O Sánchez, R Montesino, JR Toledo
    N Biotechnol, 2020;61(0):11-21.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Erythropoietin production by the kidney and the liver in response to severe hypoxia evaluated by Western blotting with deglycosylation
    Authors: Y Yasuoka, T Fukuyama, Y Izumi, Y Nakayama, H Inoue, K Yanagita, T Oshima, T Yamazaki, T Uematsu, N Kobayashi, Y Shimada, Y Nagaba, M Mukoyama, T Yamashita, Y Sato, JM Sands, K Kawahara, H Nonoguchi
    Physiol Rep, 2020;8(12):e14485.
    Species: Human
    Sample Types: Plasma
    Applications: Western Blot
  5. A human expression system based on HEK293 for the stable production of recombinant erythropoietin
    Authors: CL Chin, JB Goh, H Srinivasan, KI Liu, A Gowher, R Shanmugam, HL Lim, M Choo, WQ Tang, AH Tan, T Nguyen-Khu, MH Tan, SK Ng
    Sci Rep, 2019;9(1):16768.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Generation of transgenic chickens expressing the human erythropoietin (hEPO) gene in an oviduct-specific manner: Production of transgenic chicken eggs containing human erythropoietin in egg whites
    Authors: MS Kwon, BC Koo, D Kim, YH Nam, XS Cui, NH Kim, T Kim
    PLoS ONE, 2018;13(5):e0194721.
    Species: Chicken
    Sample Types: Protein
    Applications: Western Blot
  7. Safety and angiogenic effects of systemic gene delivery of a modified erythropoietin.
    Authors: de Lucas Cerrillo A, Bond W, Rex T
    Gene Ther, 2015;22(5):365-73.
    Species: Hamster
    Sample Types: Whole Cells
    Applications: Functional Assay
  8. EPO gene expression promotes proliferation, migration and invasion via the p38MAPK/AP-1/MMP-9 pathway by p21WAF1 expression in vascular smooth muscle cells.
    Authors: Park S, Won S, Song J, Kambe T, Nagao M, Kim W, Moon S
    Cell Signal, 2015;27(3):470-8.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. The nexus between VEGF and NFkappaB orchestrates a hypoxia-independent neovasculogenesis.
    Authors: DeNiro M, Al-Mohanna F, Alsmadi O, Al-Mohanna F
    PLoS ONE, 2013;8(3):e59021.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Quantifying Western blots: pitfalls of densitometry.
    Authors: Gassmann M, Grenacher B, Rohde B, Vogel J
    Electrophoresis, 2009;30(11):1845-55.
    Species: Bovine
    Sample Types: Plasma
    Applications: Western Blot
  11. Testing for recombinant erythropoietin.
    Authors: Delanghe JR, Bollen M, Beullens M
    Am. J. Hematol., 2008;83(3):237-41.
    Species: Human
    Sample Types: Urine
    Applications: Western Blot
  12. Novel human renal proximal tubular cell line for the production of complex proteins.
    Authors: Fliedl L, Manhart G, Kast F, Katinger H, Kunert R, Grillari J, Wieser M, Grillari-Voglauer R
    J Biotechnol, 0;176(0):29-39.

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Isotype Controls

Mouse IgG2A Isotype Control

Secondary Antibodies

Reviews for Human Erythropoietin/EPO Antibody

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Human Erythropoietin Antibody
By Leslie Priddy on 04/13/2018
Application: Immunohistochemistry Sample Tested: Serum Species: Human