Human Erythropoietin/EPO Antibody
Summary
Preparation and Storage
Reconstitution Calculator
Background
Product Datasheets
Related Research Areas
Human Erythropoietin/EPO Antibody Summary
Species Reactivity
Human
Specificity
Recognizes an epitope within the first 26 amino acids at the NH2 terminus of human urinary and human recombinant EPO. Binds human and rat EPO but does not bind sheep, baboon, or dog EPO (1, 2).
Source
Monoclonal Mouse IgG2A Clone # AE7A5
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Erythropoietin/EPO synthetic peptide analog
Ala28-Thr53
Accession # P01588
Ala28-Thr53
Accession # P01588
Formulation
Supplied as a 0.2 μm filtered solution in PBS. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated
Applications
Recommended Concentration
Sample
Immunoaffinity Purification
This antibody can be used with optimal binding of human EPO at pH 7-9 (1-3).
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Reconstitution Calculator
Preparation and Storage
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C, as supplied.
- 1 month, 2 to 8 °C under sterile conditions after opening.
- 6 months, -20 to -70 °C under sterile conditions after opening.
Background: Erythropoietin/EPO
Erythropoietin (EPO) a glycoprotein produced primarily by the kidney, is the principal factor that regulates erythropoiesis by stimulating the proliferation and differentiation of erythroid progenitor cells.
Entrez Gene IDs
2056 (Human); 13856 (Mouse); 24335 (Rat)
Alternate Names
ECYT5; EP; EPO; epoetin; Erythropoietin; MGC138142; MVCD2
Product Datasheets
Citations for Human Erythropoietin/EPO Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
13
Citations: Showing 1 - 10
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STABILON, a Novel Sequence Motif That Enhances the Expression and Accumulation of Intracellular and Secreted Proteins
Authors: Z Rethi-Nagy, E Abraham, K Udvardy, E Klement, Z Darula, M Pal, RL Katona, V Tubak, T Pali, Z Kota, R Sinka, A Udvardy, Z Lipinszki
International Journal of Molecular Sciences, 2022-07-25;23(15):.
Species: Chinese Hamster, Drosophila
Sample Types: Cell Lysates, Tisssue Homogenates
Applications: Western Blot -
Modified recombinant human erythropoietin with potentially reduced immunogenicity
Authors: T Susantad, M Fuangthong, K Tharakaram, P Tit-Oon, M Ruchirawat, R Sasisekhar
Scientific Reports, 2021-01-15;11(1):1491.
Species: Human
Sample Types: Protein
Applications: ELISA Capture -
In vivo modification of the goat mammary gland glycosylation pathway
Authors: MJ Leiva-Carr, S Jiménez-Ch, DJ Harvey, NC Parra, KC Tavares, F Camacho, A González, O Sánchez, R Montesino, JR Toledo
N Biotechnol, 2020-11-04;61(0):11-21.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Erythropoietin production by the kidney and the liver in response to severe hypoxia evaluated by Western blotting with deglycosylation
Authors: Y Yasuoka, T Fukuyama, Y Izumi, Y Nakayama, H Inoue, K Yanagita, T Oshima, T Yamazaki, T Uematsu, N Kobayashi, Y Shimada, Y Nagaba, M Mukoyama, T Yamashita, Y Sato, JM Sands, K Kawahara, H Nonoguchi
Physiol Rep, 2020-06-01;8(12):e14485.
Species: Human
Sample Types: Plasma
Applications: Western Blot -
Physicochemical and biological evaluation of JR-131 as a biosimilar to a long-acting erythropoiesis-stimulating agent darbepoetin alfa
Authors: J Tani, Y Ito, S Tatemichi, M Yamakami, T Fukui, Y Hatano, S Kakimoto, A Kotani, A Sugimura, K Mihara, R Yamamoto, N Tanaka, K Minami, K Takahashi, T Hirato
PLoS ONE, 2020-04-17;15(4):e0231830.
Species: Human
Sample Types: Protein
Applications: Western Blot -
A human expression system based on HEK293 for the stable production of recombinant erythropoietin
Authors: CL Chin, JB Goh, H Srinivasan, KI Liu, A Gowher, R Shanmugam, HL Lim, M Choo, WQ Tang, AH Tan, T Nguyen-Khu, MH Tan, SK Ng
Sci Rep, 2019-11-14;9(1):16768.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Generation of transgenic chickens expressing the human erythropoietin (hEPO) gene in an oviduct-specific manner: Production of transgenic chicken eggs containing human erythropoietin in egg whites
Authors: MS Kwon, BC Koo, D Kim, YH Nam, XS Cui, NH Kim, T Kim
PLoS ONE, 2018-05-30;13(5):e0194721.
Species: Chicken
Sample Types: Protein
Applications: Western Blot -
Safety and angiogenic effects of systemic gene delivery of a modified erythropoietin.
Authors: de Lucas Cerrillo A, Bond W, Rex T
Gene Ther, 2015-02-26;22(5):365-73.
Species: Hamster
Sample Types: Whole Cells
Applications: Functional Assay -
EPO gene expression promotes proliferation, migration and invasion via the p38MAPK/AP-1/MMP-9 pathway by p21WAF1 expression in vascular smooth muscle cells.
Authors: Park S, Won S, Song J, Kambe T, Nagao M, Kim W, Moon S
Cell Signal, 2014-12-08;27(3):470-8.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Novel human renal proximal tubular cell line for the production of complex proteins.
Authors: Fliedl L, Manhart G, Kast F, Katinger H, Kunert R, Grillari J, Wieser M, Grillari-Voglauer R
J Biotechnol, 2014-02-16;176(0):29-39.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The nexus between VEGF and NFkappaB orchestrates a hypoxia-independent neovasculogenesis.
Authors: DeNiro M, Al-Mohanna F, Alsmadi O, Al-Mohanna F
PLoS ONE, 2013-03-22;8(3):e59021.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Quantifying Western blots: pitfalls of densitometry.
Authors: Gassmann M, Grenacher B, Rohde B, Vogel J
Electrophoresis, 2009-06-01;30(11):1845-55.
Species: Bovine, Camelid, Canine, Equine, Feline, Human, Mouse, Porcine, Primate - Pongo (Orangutan), Proboscine (Elephant), Rat, Rhinoceros
Sample Types: Plasma
Applications: Western Blot -
Testing for recombinant erythropoietin.
Authors: Delanghe JR, Bollen M, Beullens M
Am. J. Hematol., 2008-03-01;83(3):237-41.
Species: Human
Sample Types: Urine
Applications: Western Blot
FAQs
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Reviews for Human Erythropoietin/EPO Antibody
Average Rating: 5 (Based on 1 Review)
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Human Erythropoietin Antibody
By Leslie Priddy
on 04/13/2018
Application: IHC
Sample Tested: Serum
Species:
Human
