Human Erythropoietin/EPO Antibody

Catalog # Availability Size / Price Qty
Product Details
Citations (13)
Supplemental Products
Reviews (1)

Human Erythropoietin/EPO Antibody Summary

Species Reactivity
Recognizes an epitope within the first 26 amino acids at the NH2 terminus of human urinary and human recombinant EPO. Binds human and rat EPO but does not bind sheep, baboon, or dog EPO (1, 2).
Monoclonal Mouse IgG2A Clone # AE7A5
Protein A or G purified from hybridoma culture supernatant
Erythropoietin/EPO synthetic peptide analog
Accession # P01588
Supplied as a 0.2 μm filtered solution in PBS. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
Recombinant Human Erythropoietin/EPO (Ultrapure) (Catalog # 286-EP)
Immunoaffinity Purification
This antibody can be used with optimal binding of human EPO at pH 7-9 (1-3).

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Background: Erythropoietin/EPO

Erythropoietin (EPO) a glycoprotein produced primarily by the kidney, is the principal factor that regulates erythropoiesis by stimulating the proliferation and differentiation of erythroid progenitor cells.

Entrez Gene IDs
2056 (Human); 13856 (Mouse); 24335 (Rat)
Alternate Names
ECYT5; EP; EPO; epoetin; Erythropoietin; MGC138142; MVCD2

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Citations for Human Erythropoietin/EPO Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. STABILON, a Novel Sequence Motif That Enhances the Expression and Accumulation of Intracellular and Secreted Proteins
    Authors: Z Rethi-Nagy, E Abraham, K Udvardy, E Klement, Z Darula, M Pal, RL Katona, V Tubak, T Pali, Z Kota, R Sinka, A Udvardy, Z Lipinszki
    International Journal of Molecular Sciences, 2022-07-25;23(15):.
    Species: Chinese Hamster, Drosophila
    Sample Types: Cell Lysates, Tisssue Homogenates
    Applications: Western Blot
  2. Modified recombinant human erythropoietin with potentially reduced immunogenicity
    Authors: T Susantad, M Fuangthong, K Tharakaram, P Tit-Oon, M Ruchirawat, R Sasisekhar
    Scientific Reports, 2021-01-15;11(1):1491.
    Species: Human
    Sample Types: Protein
    Applications: ELISA Capture
  3. In vivo modification of the goat mammary gland glycosylation pathway
    Authors: MJ Leiva-Carr, S Jiménez-Ch, DJ Harvey, NC Parra, KC Tavares, F Camacho, A González, O Sánchez, R Montesino, JR Toledo
    N Biotechnol, 2020-11-04;61(0):11-21.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Erythropoietin production by the kidney and the liver in response to severe hypoxia evaluated by Western blotting with deglycosylation
    Authors: Y Yasuoka, T Fukuyama, Y Izumi, Y Nakayama, H Inoue, K Yanagita, T Oshima, T Yamazaki, T Uematsu, N Kobayashi, Y Shimada, Y Nagaba, M Mukoyama, T Yamashita, Y Sato, JM Sands, K Kawahara, H Nonoguchi
    Physiol Rep, 2020-06-01;8(12):e14485.
    Species: Human
    Sample Types: Plasma
    Applications: Western Blot
  5. Physicochemical and biological evaluation of JR-131 as a biosimilar to a long-acting erythropoiesis-stimulating agent darbepoetin alfa
    Authors: J Tani, Y Ito, S Tatemichi, M Yamakami, T Fukui, Y Hatano, S Kakimoto, A Kotani, A Sugimura, K Mihara, R Yamamoto, N Tanaka, K Minami, K Takahashi, T Hirato
    PLoS ONE, 2020-04-17;15(4):e0231830.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  6. A human expression system based on HEK293 for the stable production of recombinant erythropoietin
    Authors: CL Chin, JB Goh, H Srinivasan, KI Liu, A Gowher, R Shanmugam, HL Lim, M Choo, WQ Tang, AH Tan, T Nguyen-Khu, MH Tan, SK Ng
    Sci Rep, 2019-11-14;9(1):16768.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Generation of transgenic chickens expressing the human erythropoietin (hEPO) gene in an oviduct-specific manner: Production of transgenic chicken eggs containing human erythropoietin in egg whites
    Authors: MS Kwon, BC Koo, D Kim, YH Nam, XS Cui, NH Kim, T Kim
    PLoS ONE, 2018-05-30;13(5):e0194721.
    Species: Chicken
    Sample Types: Protein
    Applications: Western Blot
  8. Safety and angiogenic effects of systemic gene delivery of a modified erythropoietin.
    Authors: de Lucas Cerrillo A, Bond W, Rex T
    Gene Ther, 2015-02-26;22(5):365-73.
    Species: Hamster
    Sample Types: Whole Cells
    Applications: Functional Assay
  9. EPO gene expression promotes proliferation, migration and invasion via the p38MAPK/AP-1/MMP-9 pathway by p21WAF1 expression in vascular smooth muscle cells.
    Authors: Park S, Won S, Song J, Kambe T, Nagao M, Kim W, Moon S
    Cell Signal, 2014-12-08;27(3):470-8.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Novel human renal proximal tubular cell line for the production of complex proteins.
    Authors: Fliedl L, Manhart G, Kast F, Katinger H, Kunert R, Grillari J, Wieser M, Grillari-Voglauer R
    J Biotechnol, 2014-02-16;176(0):29-39.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. The nexus between VEGF and NFkappaB orchestrates a hypoxia-independent neovasculogenesis.
    Authors: DeNiro M, Al-Mohanna F, Alsmadi O, Al-Mohanna F
    PLoS ONE, 2013-03-22;8(3):e59021.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. Quantifying Western blots: pitfalls of densitometry.
    Authors: Gassmann M, Grenacher B, Rohde B, Vogel J
    Electrophoresis, 2009-06-01;30(11):1845-55.
    Species: Bovine, Camelid, Canine, Equine, Feline, Human, Mouse, Porcine, Primate - Pongo (Orangutan), Proboscine (Elephant), Rat, Rhinoceros
    Sample Types: Plasma
    Applications: Western Blot
  13. Testing for recombinant erythropoietin.
    Authors: Delanghe JR, Bollen M, Beullens M
    Am. J. Hematol., 2008-03-01;83(3):237-41.
    Species: Human
    Sample Types: Urine
    Applications: Western Blot


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Isotype Controls

Mouse IgG2A Isotype Control

Secondary Antibodies

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Human Erythropoietin Antibody
By Leslie Priddy on 04/13/2018
Application: IHC Sample Tested: Serum Species: Human