Human Erythropoietin R Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF307
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews

Human Erythropoietin R Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Erythropoietin R in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Erythropoietin R
Pro26-Pro250
Accession # P19235
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Erythropoietin R (Catalog # 307-ER)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Erythropoietin R

Erythropoietin (Epo), a glycoprotein produced primarily by the kidney, is the principal factor that regulates erythropoiesis by stimulating the proliferation and differentiation of erythroid progenitor cells. The biological effects of Epo are mediated by the erythropoietin receptor (Epo R). The genes for human and mouse Epo R have been cloned and characterized. The full-length human Epo R cDNA encodes a type I membrane-spanning protein with 508 amino acid (aa) residues (a 24 aa residue hydrophobic signal sequence, a 226 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 236 aa residue cytoplasmic domain). At the protein sequence level, the human Epo R is approximately 82% identical to the mouse protein. As a result of alternative splicing of the Epo R gene, cDNA clones encoding a truncated form of the Epo R as well as the soluble form of Epo R has been found. The presence of a soluble form of the Epo R has also been detected on human sera. Recombinant soluble Epo R binds Epo with high affinity and is a potent Epo antagonist.

References
  1. Barber, D.L. and A.D. D’Andrea (1992) Seminars in Hematology 29:293.
  2. Youssoufian, H. et al. (1993) Blood 9:2223.
  3. Lodish, H.F. et al. (1995) Cold Spring Harbor Symposia on Quantitative Biology LX:93 - 104.
  4. Baynes, R.D. et al. (1993) Blood 82:2088.
Long Name
Erythropoietin Receptor
Entrez Gene IDs
2057 (Human); 13857 (Mouse)
Alternate Names
EpoR; EPO-R; Erythropoietin R; erythropoietin receptor; MGC138358

Product Datasheets

You must select a language.

x

Citations for Human Erythropoietin R Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

  1. Location and the functionality of erythropoietin receptor(s) in A2780 cells
    Authors: Peter Solár, Gabriela Hrčková, Lenka Varinská, Zuzana Solárová, Ján Kriška, Ivana Uhrínová et al.
    Oncology Reports
  2. Erythropoietin mimetic compound AGEM400(HES) binds to the same receptor as erythropoietin but displays a different spectrum of activities.
    Authors: Kessler C, Greindl A, Breuer B, Haberl U, Rybka A, Emgenbroich M, Frank HG, Potgens AJ
    Cytokine, 2011-12-03;57(2):226-37.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Characterization of erythropoietin receptor and erythropoietin expression and function in human ovarian cancer cells.
    Authors: Jeong JY, Feldman L, Solar P, Szenajch J, Sytkowski AJ
    Int. J. Cancer, 2008-01-15;122(2):274-80.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human Erythropoietin R Biotinylated Antibody

There are currently no reviews for this product. Be the first to review Human Erythropoietin R Biotinylated Antibody and earn rewards!

Have you used Human Erythropoietin R Biotinylated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review