Human ALK-1 Antibody

R&D Systems | Catalog # AF370

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat, Transgenic Mouse

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Surface Plasmon Resonance

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all ALK-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ALK‑1
Asp22-Gln118
Accession # P37023

Specificity

Detects human ALK-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross‑reactivity with recombinant mouse ALK-1 is observed and less than 1% cross-reactivity with recombinant human (rh) Activin RIA and rhActivin RIB is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human ALK-1 Antibody

Detection of Mouse ALK-1 by Western Blot

Detection of Mouse ALK-1 by Western Blot

GATA6 deficiency in PAEC and PASMC results in loss of BMP receptors. (A) qPCR of HPAECs transfected with GATA6 or control scr siRNA ( − ) to measure indicated mRNA, each experiment was repeated at least three times. Data are means ± SE, n = 4–7. **p < 0.01, ***p < 0.001 by Mann Whitney U test. (B) Chromatin immune precipitation (ChiP) assay in HPAECs, n = 8–10. Representative gel images and data quantification are shown. Data are means ± SE, ***p < 0.001, ****p < 0.0001 by Mann Whitney U test. (C,D) Immunoblot analysis of control human PASMC transfected with siRNA GATA6 or control scr siRNA for 48 h. Data are means ± SE, 3 subjects/group, *p < 0.05 by Mann Whitney U test. Please see Fig. 2F,G for GATA6 immunoblots. (E,F) Expression of BmpR2, Alk1, ActRIIB, and endoglin measured by qPCR in PAEC (F) and whole lungs (G) from WT and Gata6 CKO mice. Data are means ± SE; E: n = 4–5/group; F: n = 6–11 mice/group. Male and female mice responded similarly. *p < 0.05, **p < 0.01 by Mann Whitney U test ((E,F) BmpR2, Alk1, and ActRIIB) and unpaired tau test (F Endoglin). (G,H) Control HPAECs transfected with siGATA6 or control scr siRNA ( − ) were assayed by immunoblot analysis to detect indicated BMP receptors. Values are means ± SE of the relative protein levels by densitometry, n = 4–7.*p < 0.05, ***p < 0.001 by Mann Whitney U test. (I,J) Immunoblot analysis of whole lung tissue from Gata6 CKO and WT mice. Values are means ± SE of the relative protein levels by densitometry, n = 3–5/group. Male and female mice responded similarly. *p < 0.05, **p < 0.01 by Mann Whitney U test. The original blots are presented in Supplementary Fig. S17. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37087509), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse ALK-1 by Western Blot

Detection of Mouse ALK-1 by Western Blot

GATA6 deficiency in PAEC and PASMC results in loss of BMP receptors. (A) qPCR of HPAECs transfected with GATA6 or control scr siRNA ( − ) to measure indicated mRNA, each experiment was repeated at least three times. Data are means ± SE, n = 4–7. **p < 0.01, ***p < 0.001 by Mann Whitney U test. (B) Chromatin immune precipitation (ChiP) assay in HPAECs, n = 8–10. Representative gel images and data quantification are shown. Data are means ± SE, ***p < 0.001, ****p < 0.0001 by Mann Whitney U test. (C,D) Immunoblot analysis of control human PASMC transfected with siRNA GATA6 or control scr siRNA for 48 h. Data are means ± SE, 3 subjects/group, *p < 0.05 by Mann Whitney U test. Please see Fig. 2F,G for GATA6 immunoblots. (E,F) Expression of BmpR2, Alk1, ActRIIB, and endoglin measured by qPCR in PAEC (F) and whole lungs (G) from WT and Gata6 CKO mice. Data are means ± SE; E: n = 4–5/group; F: n = 6–11 mice/group. Male and female mice responded similarly. *p < 0.05, **p < 0.01 by Mann Whitney U test ((E,F) BmpR2, Alk1, and ActRIIB) and unpaired tau test (F Endoglin). (G,H) Control HPAECs transfected with siGATA6 or control scr siRNA ( − ) were assayed by immunoblot analysis to detect indicated BMP receptors. Values are means ± SE of the relative protein levels by densitometry, n = 4–7.*p < 0.05, ***p < 0.001 by Mann Whitney U test. (I,J) Immunoblot analysis of whole lung tissue from Gata6 CKO and WT mice. Values are means ± SE of the relative protein levels by densitometry, n = 3–5/group. Male and female mice responded similarly. *p < 0.05, **p < 0.01 by Mann Whitney U test. The original blots are presented in Supplementary Fig. S17. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37087509), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human ALK-1 by Western Blot

Detection of Human ALK-1 by Western Blot

GATA6 deficiency in PAEC and PASMC results in loss of BMP receptors. (A) qPCR of HPAECs transfected with GATA6 or control scr siRNA ( − ) to measure indicated mRNA, each experiment was repeated at least three times. Data are means ± SE, n = 4–7. **p < 0.01, ***p < 0.001 by Mann Whitney U test. (B) Chromatin immune precipitation (ChiP) assay in HPAECs, n = 8–10. Representative gel images and data quantification are shown. Data are means ± SE, ***p < 0.001, ****p < 0.0001 by Mann Whitney U test. (C,D) Immunoblot analysis of control human PASMC transfected with siRNA GATA6 or control scr siRNA for 48 h. Data are means ± SE, 3 subjects/group, *p < 0.05 by Mann Whitney U test. Please see Fig. 2F,G for GATA6 immunoblots. (E,F) Expression of BmpR2, Alk1, ActRIIB, and endoglin measured by qPCR in PAEC (F) and whole lungs (G) from WT and Gata6 CKO mice. Data are means ± SE; E: n = 4–5/group; F: n = 6–11 mice/group. Male and female mice responded similarly. *p < 0.05, **p < 0.01 by Mann Whitney U test ((E,F) BmpR2, Alk1, and ActRIIB) and unpaired tau test (F Endoglin). (G,H) Control HPAECs transfected with siGATA6 or control scr siRNA ( − ) were assayed by immunoblot analysis to detect indicated BMP receptors. Values are means ± SE of the relative protein levels by densitometry, n = 4–7.*p < 0.05, ***p < 0.001 by Mann Whitney U test. (I,J) Immunoblot analysis of whole lung tissue from Gata6 CKO and WT mice. Values are means ± SE of the relative protein levels by densitometry, n = 3–5/group. Male and female mice responded similarly. *p < 0.05, **p < 0.01 by Mann Whitney U test. The original blots are presented in Supplementary Fig. S17. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37087509), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human ALK-1 by Western Blot

Detection of Human ALK-1 by Western Blot

GATA6 deficiency in PAEC and PASMC results in loss of BMP receptors. (A) qPCR of HPAECs transfected with GATA6 or control scr siRNA ( − ) to measure indicated mRNA, each experiment was repeated at least three times. Data are means ± SE, n = 4–7. **p < 0.01, ***p < 0.001 by Mann Whitney U test. (B) Chromatin immune precipitation (ChiP) assay in HPAECs, n = 8–10. Representative gel images and data quantification are shown. Data are means ± SE, ***p < 0.001, ****p < 0.0001 by Mann Whitney U test. (C,D) Immunoblot analysis of control human PASMC transfected with siRNA GATA6 or control scr siRNA for 48 h. Data are means ± SE, 3 subjects/group, *p < 0.05 by Mann Whitney U test. Please see Fig. 2F,G for GATA6 immunoblots. (E,F) Expression of BmpR2, Alk1, ActRIIB, and endoglin measured by qPCR in PAEC (F) and whole lungs (G) from WT and Gata6 CKO mice. Data are means ± SE; E: n = 4–5/group; F: n = 6–11 mice/group. Male and female mice responded similarly. *p < 0.05, **p < 0.01 by Mann Whitney U test ((E,F) BmpR2, Alk1, and ActRIIB) and unpaired tau test (F Endoglin). (G,H) Control HPAECs transfected with siGATA6 or control scr siRNA ( − ) were assayed by immunoblot analysis to detect indicated BMP receptors. Values are means ± SE of the relative protein levels by densitometry, n = 4–7.*p < 0.05, ***p < 0.001 by Mann Whitney U test. (I,J) Immunoblot analysis of whole lung tissue from Gata6 CKO and WT mice. Values are means ± SE of the relative protein levels by densitometry, n = 3–5/group. Male and female mice responded similarly. *p < 0.05, **p < 0.01 by Mann Whitney U test. The original blots are presented in Supplementary Fig. S17. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37087509), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human ALK-1 Antibody

Application
Recommended Usage

Immunoprecipitation

David, L. et al. (2007) Blood. 109:1953.

Western Blot

0.1 µg/mL
Sample: Recombinant Human ALK‑1 Fc Chimera (Catalog # 370-AL)

Reviewed Applications

Read 2 reviews rated 5 using AF370 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ALK-1

Transforming Growth Factor beta (TGF-beta ) superfamily ligands exert their biological activities via binding to heteromeric receptor complexes of two types (I and II) of serine/threonine kinases. Type II receptors are constitutively active kinases that phosphorylate type I receptors upon ligand binding. In turn, activated type I kinases phosphorylate downstream signaling molecules including the various smads. Transmembrane proteoglycans, including the type III receptor (betaglycan) and endoglin, can bind and present some of the TGF-beta superfamily ligands to type I and II receptor complexes and enhance their cellular responses. Seven type I receptors (also termed activin receptor-like kinase (ALK)) and five type II receptors have been isolated from mammals. ALK-2, -3, -4, -5, and -6 are also known as Activin R1A, BMPR-1A, Activin R1B, TGF-beta R1, and BMPR-1B, respectively, reflecting their ligand preferences. Evidence suggests that TGF-beta 1, TGF-beta 3 and an unknown ligand present in serum can activate chimeric ALK-1. ALK-1 shares with other type I receptors a cysteine-rich domain with conserved cysteine spacing in the extracellular region, and a glycine- and serine-rich domain (the GS domain) preceding the kinase domain. ALK-1 is expressed highly in endothelial cells and other highly vascularized tissues. The expression patterns of ALK-1 parallels that of endoglin. Mutations in ALK-1 as well as in endoglin are associated with hereditary hemorrhagic telangiectasia (HHT), suggesting a critical role for ALK-1 in the control of blood vessel development or repair. Human and mouse ALK-1 share approximately 71% amino acid sequence identity in their extracellular regions.

References

  1. ten Dijke, P. et al. (1993) Oncogene 8:2879.
  2. ten Dijke, P. et al. (1994) Science 264:101.
  3. Lux, A. et al. (1999) J. Biol. Chem. 274:9984.

Long Name

Activin Receptor-like Kinase 1

Alternate Names

ACVRL1, ALK1

Entrez Gene IDs

94 (Human); 11482 (Mouse)

Gene Symbol

ACVRL1

UniProt

P37023

Additional ALK-1 Products

Product Documents for Human ALK-1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human ALK-1 Antibody

For research use only

Citations for Human ALK-1 Antibody

Customer Reviews for Human ALK-1 Antibody (2)

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  • Human ALK-1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Human lung cancer line H1299 and NCI-H1650 human non-small cell lung cancer cell line
    Species: Human
    Verified Customer | Posted 04/04/2020
    Human ALK-1 Antibody AF370
  • Human ALK-1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Calu-6 human lung carcinoma cell line and HCC827 human non-small cell lung cancer cell line
    Species: Human
    Verified Customer | Posted 10/17/2018
    Human ALK-1 Antibody AF370

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Associated Pathways

TGF-beta Signaling Pathways
TGF-beta Signaling Pathway Thumbnail